5hz2 - Revision history

OCA at 11:01, 16 August 2023

OCA — Wed, 16 Aug 2023 11:01:02 GMT

←Older revision		Revision as of 11:01, 16 August 2023
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	<StructureSection load='5hz2' size='340' side='right'caption='[[5hz2]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5hz2' size='340' side='right'caption='[[5hz2]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hz2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Alcaligenes_eutropha_h16 Alcaligenes eutropha h16~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZ2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HZ2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hz2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator_H16 Cupriavidus necator H16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HZ2 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~phbC, H16_A1437 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=381666 Alcaligenes eutropha H16])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hz2 OCA], [~~http~~://pdbe.org/5hz2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hz2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hz2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hz2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hz2 OCA], [https://pdbe.org/5hz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hz2 RCSB], [https://www.ebi.ac.uk/pdbsum/5hz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hz2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~PHBC_CUPNH PHBC_CUPNH]~~] Polymerizes (R)-3-hydroxybutyryl-CoA to create polyhydroxybutyrate (PHB) which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation.<ref>PMID:2670936</ref>	+	[https://www.uniprot.org/uniprot/PHAC_CUPNH PHAC_CUPNH] Polymerizes (R)-3-hydroxybutyryl-CoA to create polyhydroxybutyrate (PHB) which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation.<ref>PMID:2670936</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alcaligenes eutropha h16~~]]	+	[[Category: Cupriavidus necator H16]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kim, J]]	+	[[Category: Kim J]]
-	[[Category: Kim, K J]]	+	[[Category: Kim K-J]]
-	~~[[Category: Transferase~~]]	+

OCA at 08:37, 27 May 2020

OCA — Wed, 27 May 2020 08:37:37 GMT

←Older revision		Revision as of 08:37, 27 May 2020
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	==Crystal structure of PhaC1 from Ralstonia eutropha==		==Crystal structure of PhaC1 from Ralstonia eutropha==
-	<StructureSection load='5hz2' size='340' side='right' caption='[[5hz2]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5hz2' size='340' side='right'caption='[[5hz2]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hz2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZ2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HZ2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hz2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_eutropha_h16 Alcaligenes eutropha h16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZ2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HZ2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hz2 OCA], [http://pdbe.org/5hz2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hz2 RCSB], [http://www.ebi.ac.uk/pdbsum/5hz2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hz2 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phbC, H16_A1437 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hz2 OCA], [http://pdbe.org/5hz2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hz2 RCSB], [http://www.ebi.ac.uk/pdbsum/5hz2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hz2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Alcaligenes eutropha h16]]
		+	[[Category: Large Structures]]
	[[Category: Kim, J]]		[[Category: Kim, J]]
	[[Category: Kim, K J]]		[[Category: Kim, K J]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 06:39, 5 April 2017

OCA — Wed, 05 Apr 2017 06:39:40 GMT

←Older revision		Revision as of 06:39, 5 April 2017
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	</div>		</div>
	<div class="pdbe-citations 5hz2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5hz2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Polyhydroxybutyrate synthase\|Polyhydroxybutyrate synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:34, 9 December 2016

OCA — Fri, 09 Dec 2016 19:34:13 GMT

←Older revision		Revision as of 19:34, 9 December 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5hz2 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of PhaC1 from Ralstonia eutropha==
		+	<StructureSection load='5hz2' size='340' side='right' caption='[[5hz2]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5hz2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HZ2 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hz2 OCA], [http://pdbe.org/5hz2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hz2 RCSB], [http://www.ebi.ac.uk/pdbsum/5hz2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hz2 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PHBC_CUPNH PHBC_CUPNH]] Polymerizes (R)-3-hydroxybutyryl-CoA to create polyhydroxybutyrate (PHB) which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation.<ref>PMID:2670936</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polyhydroxyalkanoates (PHAs) are natural polyesters synthesized by numerous microorganisms as energy and reducing power storage materials, and have attracted much attention as substitutes for petroleum-based plastics. Here, we report the first crystal structure of Ralstonia eutropha PHA synthase at 1.8 A resolution and structure-based mechanisms for PHA polymerization. RePhaC1 contains two distinct domains, the N-terminal (RePhaC1ND ) and C-terminal domains (RePhaC1CD ), and exists as a dimer. RePhaC1CD catalyzes polymerization via non-processive ping-pong mechanism using a Cys-His-Asp catalytic triad. Molecular docking simulation of 3-hydroxybutyryl-CoA to the active site of RePhaC1CD reveals residues involved in the formation of 3-hydroxybutyryl-CoA binding pocket and substrate binding tunnel. Comparative analysis with other polymerases elucidates how different classes of PHA synthases show different substrate specificities. Furthermore, we attempted structure-based protein engineering and developed a RePhaC1 mutant with enhanced PHA synthase activity.

-	~~Authors: Kim~~, J., Kim, ~~K.-~~J.	+	Crystal structure of Ralstonia eutropha polyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms.,Kim J, Kim YJ, Choi SY, Lee SY, Kim KJ Biotechnol J. 2016 Nov 3. doi: 10.1002/biot.201600648. PMID:27808482<ref>PMID:27808482</ref>

-	~~Description: Crystal structure~~ of ~~PhaC1 from Ralstonia eutropha~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category: Kim, K.~~-~~J]]~~	+	<div class="pdbe-citations 5hz2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Kim, J]]		[[Category: Kim, J]]
		+	[[Category: Kim, K J]]
		+	[[Category: Transferase]]

OCA at 13:11, 2 March 2016

OCA — Wed, 02 Mar 2016 13:11:13 GMT

←Older revision		Revision as of 13:11, 2 March 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hz2 is ON HOLD	+	The entry 5hz2 is ON HOLD until Paper Publication

	Authors: Kim, J., Kim, K.-J.		Authors: Kim, J., Kim, K.-J.

OCA at 01:20, 21 February 2016

OCA — Sun, 21 Feb 2016 01:20:29 GMT

←Older revision		Revision as of 01:20, 21 February 2016
Line 3:		Line 3:
	The entry 5hz2 is ON HOLD		The entry 5hz2 is ON HOLD

-	Authors: Kim, J, Kim, K.-J.	+	Authors: Kim, J., Kim, K.-J.

	Description: Crystal structure of PhaC1 from Ralstonia eutropha		Description: Crystal structure of PhaC1 from Ralstonia eutropha
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Kim, J, Kim, ~~K.-~~J]]	+	[[Category: Kim, K.-J]]
		+	[[Category: Kim, J]]

OCA: Protected "5hz2" [edit=sysop:move=sysop]

OCA — Wed, 10 Feb 2016 15:56:29 GMT

Protected "5hz2" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:56, 10 February 2016

OCA: New page: '''Unreleased structure''' The entry 5hz2 is ON HOLD Authors: Kim, J, Kim, K.-J. Description: Crystal structure of PhaC1 from Ralstonia eutropha Category: Unreleased Structures [[C...

OCA — Wed, 10 Feb 2016 15:56:28 GMT

New page: '''Unreleased structure''' The entry 5hz2 is ON HOLD Authors: Kim, J, Kim, K.-J. Description: Crystal structure of PhaC1 from Ralstonia eutropha Category: Unreleased Structures [[C...

New page

'''Unreleased structure'''

The entry 5hz2 is ON HOLD

Authors: Kim, J, Kim, K.-J.

Description: Crystal structure of PhaC1 from Ralstonia eutropha
[[Category: Unreleased Structures]]
[[Category: Kim, J, Kim, K.-J]]