5hzf - Revision history

OCA at 11:01, 16 August 2023

OCA — Wed, 16 Aug 2023 11:01:23 GMT

←Older revision		Revision as of 11:01, 16 August 2023
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	<StructureSection load='5hzf' size='340' side='right'caption='[[5hzf]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='5hzf' size='340' side='right'caption='[[5hzf]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hzf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HZF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hzf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HZF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5hcf\|5hcf]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">C1QB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzf OCA], [https://pdbe.org/5hzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hzf RCSB], [https://www.ebi.ac.uk/pdbsum/5hzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hzf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzf OCA], [~~http~~://pdbe.org/5hzf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hzf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hzf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hzf ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/C1QA_HUMAN C1QA_HUMAN]] Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:[~~http~~://omim.org/entry/613652 613652]]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.	+	[https://www.uniprot.org/uniprot/C1QA_HUMAN C1QA_HUMAN] Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:[https://omim.org/entry/613652 613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.[https://www.uniprot.org/uniprot/C1QC_HUMAN C1QC_HUMAN] Defects in C1QC are a cause of complement component C1q deficiency (C1QD) [MIM:[https://omim.org/entry/613652 613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.<ref>PMID:8630118</ref> [https://www.uniprot.org/uniprot/C1QB_HUMAN C1QB_HUMAN] Defects in C1QB are a cause of complement component C1q deficiency (C1QD) [MIM:[https://omim.org/entry/613652 613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.<ref>PMID:9476130</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/C1QA_HUMAN C1QA_HUMAN]] C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.	+	[https://www.uniprot.org/uniprot/C1QA_HUMAN C1QA_HUMAN] C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.[https://www.uniprot.org/uniprot/C1QC_HUMAN C1QC_HUMAN] C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.[https://www.uniprot.org/uniprot/C1QB_HUMAN C1QB_HUMAN] C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gaboriaud~~, C P~~]]	+	[[Category: Gaboriaud CP]]
-	[[Category: Moreau~~, C P]]~~	+	[[Category: Moreau CP]]
-	~~[[Category: C1q]]~~	+
-	~~[[Category: Complement]]~~	+
-	~~[[Category: Gc1q domain]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 05:56, 12 June 2019

OCA — Wed, 12 Jun 2019 05:56:25 GMT

←Older revision		Revision as of 05:56, 12 June 2019
Line 1:		Line 1:

	==Single Chain Recombinant Globular Head of the Complement System Protein C1q in complex with magnesium==		==Single Chain Recombinant Globular Head of the Complement System Protein C1q in complex with magnesium==
-	<StructureSection load='5hzf' size='340' side='right' caption='[[5hzf]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='5hzf' size='340' side='right'caption='[[5hzf]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hzf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HZF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hzf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HZF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hcf\|5hcf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hcf\|5hcf]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C1QB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzf OCA], [http://pdbe.org/5hzf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hzf RCSB], [http://www.ebi.ac.uk/pdbsum/5hzf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hzf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzf OCA], [http://pdbe.org/5hzf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hzf RCSB], [http://www.ebi.ac.uk/pdbsum/5hzf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hzf ProSAT]</span></td></tr>
	</table>		</table>
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~GABORIAUD~~, C P]]	+	[[Category: Human]]
-	[[Category: ~~MOREAU~~, C P]]	+	[[Category: Large Structures]]
		+	[[Category: Gaboriaud, C P]]
		+	[[Category: Moreau, C P]]
	[[Category: C1q]]		[[Category: C1q]]
	[[Category: Complement]]		[[Category: Complement]]
	[[Category: Gc1q domain]]		[[Category: Gc1q domain]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 04:17, 30 August 2017

OCA — Wed, 30 Aug 2017 04:17:28 GMT

←Older revision		Revision as of 04:17, 30 August 2017
Line 6:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hcf\|5hcf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hcf\|5hcf]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzf OCA], [http://pdbe.org/5hzf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hzf RCSB], [http://www.ebi.ac.uk/pdbsum/5hzf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzf OCA], [http://pdbe.org/5hzf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hzf RCSB], [http://www.ebi.ac.uk/pdbsum/5hzf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hzf ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA at 18:30, 10 May 2016

OCA — Tue, 10 May 2016 18:30:06 GMT

←Older revision		Revision as of 18:30, 10 May 2016
Line 12:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/C1QA_HUMAN C1QA_HUMAN]] C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.		[[http://www.uniprot.org/uniprot/C1QA_HUMAN C1QA_HUMAN]] C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Complement C1q is a soluble pattern recognition molecule comprising six heterotrimeric subunits assembled from three polypeptide chains (A-C). Each heterotrimer forms a collagen-like stem prolonged by a globular recognition domain. These recognition domains sense a wide variety of ligands, including pathogens and altered-self components. Ligand recognition is either direct or mediated by immunoglobulins or pentraxins. Multivalent binding of C1q to its targets triggers immune effector mechanisms mediated via its collagen-like stems. The induced immune response includes activation of the classical complement pathway and enhancement of the phagocytosis of the recognized target. We report here, the first production of a single-chain recombinant form of human C1q globular region (C1q-scGR). The three monomers have been linked in tandem to generate a single continuous polypeptide, based on a strategy previously used for adiponectin, a protein structurally related to C1q. The resulting C1q-scGR protein was produced at high yield in stably transfected 293-F mammalian cells. Recombinant C1q-scGR was correctly folded, as demonstrated by its X-ray crystal structure solved at a resolution of 1.35 A. Its interaction properties were assessed by surface plasmon resonance analysis using the following physiological C1q ligands: the receptor for C1q globular heads, the long pentraxin PTX3, calreticulin, and heparin. The 3D structure and the binding properties of C1q-scGR were similar to those of the three-chain fragment generated by collagenase digestion of serum-derived C1q. Comparison of the interaction properties of the fragments with those of native C1q provided insights into the avidity component associated with the hexameric assembly of C1q. The interest of this functional recombinant form of the recognition domains of C1q in basic research and its potential biomedical applications are discussed.
		+
		+	Structural and Functional Characterization of a Single-Chain Form of the Recognition Domain of Complement Protein C1q.,Moreau C, Bally I, Chouquet A, Bottazzi B, Ghebrehiwet B, Gaboriaud C, Thielens N Front Immunol. 2016 Mar 2;7:79. doi: 10.3389/fimmu.2016.00079. eCollection 2016. PMID:26973654<ref>PMID:26973654</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hzf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:30, 2 March 2016

OCA — Wed, 02 Mar 2016 15:30:34 GMT

←Older revision		Revision as of 15:30, 2 March 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hzf is ~~ON HOLD until Paper Publication~~	+	==Single Chain Recombinant Globular Head of the Complement System Protein C1q in complex with magnesium==
-		+	<StructureSection load='5hzf' size='340' side='right' caption='[[5hzf]], [[Resolution\|resolution]] 1.55Å' scene=''>
-	~~Authors~~: ~~MOREAU~~, C.P., ~~GABORIAUD~~, C.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5hzf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HZF FirstGlance]. <br>
-	~~Description~~: ~~Single Chain Recombinant Globular Head~~ of ~~the Complement System Protein~~ C1q in complex with ~~magnesium~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hcf\|5hcf]]</td></tr>
-	[[Category: ~~Gaboriaud~~, C.P]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzf OCA], [http://pdbe.org/5hzf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hzf RCSB], [http://www.ebi.ac.uk/pdbsum/5hzf PDBsum]</span></td></tr>
-	[[Category: ~~Moreau~~, C.P]]	+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/C1QA_HUMAN C1QA_HUMAN]] Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:[http://omim.org/entry/613652 613652]]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/C1QA_HUMAN C1QA_HUMAN]] C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: GABORIAUD, C P]]
		+	[[Category: MOREAU, C P]]
		+	[[Category: C1q]]
		+	[[Category: Complement]]
		+	[[Category: Gc1q domain]]
		+	[[Category: Signaling protein]]

OCA at 01:21, 21 February 2016

OCA — Sun, 21 Feb 2016 01:21:10 GMT

←Older revision		Revision as of 01:21, 21 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hzf is ON HOLD	+	The entry 5hzf is ON HOLD until Paper Publication

	Authors: MOREAU, C.P., GABORIAUD, C.P.		Authors: MOREAU, C.P., GABORIAUD, C.P.

OCA: Protected "5hzf" [edit=sysop:move=sysop]

OCA — Wed, 10 Feb 2016 15:57:36 GMT

Protected "5hzf" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:57, 10 February 2016

OCA: New page: '''Unreleased structure''' The entry 5hzf is ON HOLD Authors: MOREAU, C.P., GABORIAUD, C.P. Description: Single Chain Recombinant Globular Head of the Complement System Protein C1q in ...

OCA — Wed, 10 Feb 2016 15:57:34 GMT

New page: '''Unreleased structure''' The entry 5hzf is ON HOLD Authors: MOREAU, C.P., GABORIAUD, C.P. Description: Single Chain Recombinant Globular Head of the Complement System Protein C1q in ...

New page

'''Unreleased structure'''

The entry 5hzf is ON HOLD

Authors: MOREAU, C.P., GABORIAUD, C.P.

Description: Single Chain Recombinant Globular Head of the Complement System Protein C1q in complex with magnesium
[[Category: Unreleased Structures]]
[[Category: Gaboriaud, C.P]]
[[Category: Moreau, C.P]]