5i34 - Revision history

OCA at 08:21, 23 August 2023

OCA — Wed, 23 Aug 2023 08:21:32 GMT

←Older revision		Revision as of 08:21, 23 August 2023
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	<StructureSection load='5i34' size='340' side='right'caption='[[5i34]], [[Resolution\|resolution]] 1.53Å' scene=''>		<StructureSection load='5i34' size='340' side='right'caption='[[5i34]], [[Resolution\|resolution]] 1.53Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i34]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Crynh Crynh~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I34 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5I34 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i34]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptococcus_neoformans_var._grubii_H99 Cryptococcus neoformans var. grubii H99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I34 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GDP:GUANOSINE~~-~~5-DIPHOSPHATE'>GDP</scene>~~, ~~<scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.53Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[5i33\|5i33~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CNAG_02858 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~235443 CRYNH])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i34 OCA], [https://pdbe.org/5i34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i34 RCSB], [https://www.ebi.ac.uk/pdbsum/5i34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i34 ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5i34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i34 OCA], [~~http~~://pdbe.org/5i34 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5i34 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5i34 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5i34 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/J9VI09_CRYNH J9VI09_CRYNH]] Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.[HAMAP-Rule:MF_03125] Plays an important role in the de novo pathway of purine nucleotide biosynthesis.[RuleBase:RU000520]	+	[https://www.uniprot.org/uniprot/J9VI09_CRYNH J9VI09_CRYNH] Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.[HAMAP-Rule:MF_03125] Plays an important role in the de novo pathway of purine nucleotide biosynthesis.[RuleBase:RU000520]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Adenylosuccinate synthase]]~~	+	[[Category: Cryptococcus neoformans var. grubii H99]]
-	~~[[Category: Crynh~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blundell~~, R D~~]]	+	[[Category: Blundell RD]]
-	[[Category: Ericsson, D]]	+	[[Category: Ericsson D]]
-	[[Category: Fraser~~, J A~~]]	+	[[Category: Fraser JA]]
-	[[Category: Kobe, B]]	+	[[Category: Kobe B]]
-	[[Category: Williams~~, S J]]~~	+	[[Category: Williams SJ]]
-	~~[[Category: Adenylosuccinate synthetase]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Purine metabolism~~]]	+

OCA at 08:43, 27 May 2020

OCA — Wed, 27 May 2020 08:43:35 GMT

←Older revision		Revision as of 08:43, 27 May 2020
Line 1:		Line 1:

	==Adenylosuccinate synthetase from Cryptococcus neoformans complexed with GDP and IMP==		==Adenylosuccinate synthetase from Cryptococcus neoformans complexed with GDP and IMP==
-	<StructureSection load='5i34' size='340' side='right' caption='[[5i34]], [[Resolution\|resolution]] 1.53Å' scene=''>	+	<StructureSection load='5i34' size='340' side='right'caption='[[5i34]], [[Resolution\|resolution]] 1.53Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i34]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I34 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5I34 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i34]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crynh Crynh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I34 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5I34 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i33\|5i33]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i33\|5i33]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CNAG_02858 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235443 CRYNH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5i34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i34 OCA], [http://pdbe.org/5i34 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i34 RCSB], [http://www.ebi.ac.uk/pdbsum/5i34 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i34 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5i34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i34 OCA], [http://pdbe.org/5i34 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i34 RCSB], [http://www.ebi.ac.uk/pdbsum/5i34 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i34 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:

	==See Also==		==See Also==
-	*[[Adenylosuccinate ~~Synthetase~~\|Adenylosuccinate ~~Synthetase~~]]	+	*[[Adenylosuccinate synthetase 3D structures\|Adenylosuccinate synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Adenylosuccinate synthase]]		[[Category: Adenylosuccinate synthase]]
		+	[[Category: Crynh]]
		+	[[Category: Large Structures]]
	[[Category: Blundell, R D]]		[[Category: Blundell, R D]]
	[[Category: Ericsson, D]]		[[Category: Ericsson, D]]

OCA at 03:37, 10 December 2016

OCA — Sat, 10 Dec 2016 03:37:42 GMT

←Older revision		Revision as of 03:37, 10 December 2016
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/J9VI09_CRYNH J9VI09_CRYNH]] Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.[HAMAP-Rule:MF_03125] Plays an important role in the de novo pathway of purine nucleotide biosynthesis.[RuleBase:RU000520]		[[http://www.uniprot.org/uniprot/J9VI09_CRYNH J9VI09_CRYNH]] Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.[HAMAP-Rule:MF_03125] Plays an important role in the de novo pathway of purine nucleotide biosynthesis.[RuleBase:RU000520]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Opportunistic fungal pathogens such as Cryptococcus neoformans are a growing cause of morbidity and mortality among immunocompromised populations worldwide. To address the current paucity of antifungal therapeutic agents, further research into fungal-specific drug targets is required. Adenylosuccinate synthetase (AdSS) is a crucial enzyme in the adeosine triphosphate (ATP) biosynthetic pathway, catalyzing the formation of adenylosuccinate from inosine monophosphate and aspartate. We have investigated the potential of this enzyme as an antifungal drug target, finding that loss of function results in adenine auxotrophy in C. neoformans, as well as complete loss of virulence in a murine model. Cryptococcal AdSS was expressed and purified in Escherichia coli and the enzyme's crystal structure determined, the first example of a structure of this enzyme from fungi. Together with enzyme kinetic studies, this structural information enabled comparison of the fungal enzyme with the human orthologue and revealed species-specific differences potentially exploitable via rational drug design. These results validate AdSS as a promising antifungal drug target and lay a foundation for future in silico and in vitro screens for novel antifungal compounds.
		+
		+	Disruption of de Novo Adenosine Triphosphate (ATP) Biosynthesis Abolishes Virulence in Cryptococcus neoformans.,Blundell RD, Williams SJ, Arras SD, Chitty JL, Blake KL, Ericsson DJ, Tibrewal N, Rohr J, Koh YQ, Kappler U, Robertson AA, Butler MS, Cooper MA, Kobe B, Fraser JA ACS Infect Dis. 2016 Sep 9;2(9):651-663. Epub 2016 Aug 17. PMID:27759389<ref>PMID:27759389</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5i34" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Adenylosuccinate Synthetase\|Adenylosuccinate Synthetase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:51, 10 September 2016

OCA — Sat, 10 Sep 2016 06:51:37 GMT

←Older revision		Revision as of 06:51, 10 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5i34 is ~~ON HOLD until Paper Publication~~	+	==Adenylosuccinate synthetase from Cryptococcus neoformans complexed with GDP and IMP==
-		+	<StructureSection load='5i34' size='340' side='right' caption='[[5i34]], [[Resolution\|resolution]] 1.53Å' scene=''>
-	~~Authors~~: ~~Blundell~~, R.D., ~~Williams, S~~.J., ~~Ericsson~~, D., ~~Fraser, J~~.A., ~~Kobe, B~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5i34]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I34 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I34 FirstGlance]. <br>
-	~~Description~~: ~~Adenylosuccinate synthetase from Cryptococcus neoformans complexed with GDP~~ and IMP	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i33\|5i33]]</td></tr>
-	[[Category: Fraser, J.A]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i34 OCA], [http://pdbe.org/5i34 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i34 RCSB], [http://www.ebi.ac.uk/pdbsum/5i34 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i34 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/J9VI09_CRYNH J9VI09_CRYNH]] Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.[HAMAP-Rule:MF_03125] Plays an important role in the de novo pathway of purine nucleotide biosynthesis.[RuleBase:RU000520]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Adenylosuccinate synthase]]
		+	[[Category: Blundell, R D]]
		+	[[Category: Ericsson, D]]
		+	[[Category: Fraser, J A]]
	[[Category: Kobe, B]]		[[Category: Kobe, B]]
-	[[Category: Williams, S.J]]	+	[[Category: Williams, S J]]
-	[[Category: ~~Ericsson, D~~]]	+	[[Category: Adenylosuccinate synthetase]]
-	[[Category: ~~Blundell, R.D~~]]	+	[[Category: Dimer]]
		+	[[Category: Ligase]]
		+	[[Category: Purine metabolism]]

OCA at 00:09, 11 May 2016

OCA — Wed, 11 May 2016 00:09:54 GMT

←Older revision		Revision as of 00:09, 11 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5i34 is ON HOLD	+	The entry 5i34 is ON HOLD until Paper Publication

	Authors: Blundell, R.D., Williams, S.J., Ericsson, D., Fraser, J.A., Kobe, B.		Authors: Blundell, R.D., Williams, S.J., Ericsson, D., Fraser, J.A., Kobe, B.

OCA at 16:41, 26 February 2016

OCA — Fri, 26 Feb 2016 16:41:53 GMT

←Older revision		Revision as of 16:41, 26 February 2016
Line 3:		Line 3:
	The entry 5i34 is ON HOLD		The entry 5i34 is ON HOLD

-	Authors: Blundell, RD., Williams, SJ., Ericsson, D., Fraser, JA, Kobe, B.	+	Authors: Blundell, R.D., Williams, S.J., Ericsson, D., Fraser, J.A., Kobe, B.

	Description: Adenylosuccinate synthetase from Cryptococcus neoformans complexed with GDP and IMP		Description: Adenylosuccinate synthetase from Cryptococcus neoformans complexed with GDP and IMP
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Fraser, ~~Ja,~~ Kobe, B]]	+	[[Category: Fraser, J.A]]
-	[[Category: Williams, Sj]]	+	[[Category: Kobe, B]]
		+	[[Category: Williams, S.J]]
	[[Category: Ericsson, D]]		[[Category: Ericsson, D]]
-	[[Category: Blundell, Rd]]	+	[[Category: Blundell, R.D]]

OCA: Protected "5i34" [edit=sysop:move=sysop]

OCA — Sun, 21 Feb 2016 01:31:19 GMT

Protected "5i34" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:31, 21 February 2016

OCA: New page: '''Unreleased structure''' The entry 5i34 is ON HOLD Authors: Blundell, RD., Williams, SJ., Ericsson, D., Fraser, JA, Kobe, B. Description: Adenylosuccinate synthetase from Cryptococcu...

OCA — Sun, 21 Feb 2016 01:31:18 GMT

New page: '''Unreleased structure''' The entry 5i34 is ON HOLD Authors: Blundell, RD., Williams, SJ., Ericsson, D., Fraser, JA, Kobe, B. Description: Adenylosuccinate synthetase from Cryptococcu...

New page

'''Unreleased structure'''

The entry 5i34 is ON HOLD

Authors: Blundell, RD., Williams, SJ., Ericsson, D., Fraser, JA, Kobe, B.

Description: Adenylosuccinate synthetase from Cryptococcus neoformans complexed with GDP and IMP
[[Category: Unreleased Structures]]
[[Category: Fraser, Ja, Kobe, B]]
[[Category: Williams, Sj]]
[[Category: Ericsson, D]]
[[Category: Blundell, Rd]]