5i4c - Revision history

OCA at 12:33, 6 March 2024

OCA — Wed, 06 Mar 2024 12:33:44 GMT

←Older revision		Revision as of 12:33, 6 March 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RCSB_ECOLI RCSB_ECOLI] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981]<ref>PMID:10702265</ref> <ref>PMID:11309126</ref> <ref>PMID:11566985</ref> <ref>PMID:13129944</ref> <ref>PMID:1597415</ref> <ref>PMID:20189963</ref> <ref>PMID:20952573</ref>		[https://www.uniprot.org/uniprot/RCSB_ECOLI RCSB_ECOLI] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981]<ref>PMID:10702265</ref> <ref>PMID:11309126</ref> <ref>PMID:11566985</ref> <ref>PMID:13129944</ref> <ref>PMID:1597415</ref> <ref>PMID:20189963</ref> <ref>PMID:20952573</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	RcsB, the transcription-associated response regulator of the Rcs phosphorelay two-component signal transduction system, activates cell stress responses associated with desiccation, cell wall biosynthesis, cell division, virulence, biofilm formation and antibiotic resistance in enteric bacterial pathogens. RcsB belongs to the FixJ/NarL family of transcriptional regulators, which are characterized by a highly conserved C-terminal DNA-binding domain. The N-terminal domain of RcsB belongs to the family of two-component receiver domains. This receiver domain contains the phosphoacceptor site and participates in RcsB dimer formation; it also contributes to dimer formation with other transcription factor partners. Here, we describe the crystal structure of the Escherichia coli RcsB receiver domain in its non-phosphorylated state. The structure reveals important molecular details of phosphorylation-independent dimerization of RcsB and has implication for the formation of heterodimers. This article is protected by copyright. All rights reserved.
-
-	Crystal structure of non-phosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli.,Filippova EV, Wawrzak Z, Ruan J, Pshenychnyi S, Schultz RM, Wolfe AJ, Anderson WF Protein Sci. 2016 Sep 27. doi: 10.1002/pro.3050. PMID:27670836<ref>PMID:27670836</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5i4c" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 08:22, 23 August 2023

OCA — Wed, 23 Aug 2023 08:22:45 GMT

←Older revision		Revision as of 08:22, 23 August 2023
Line 1:		Line 1:

	==Crystal structure of non-phosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli==		==Crystal structure of non-phosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli==
-	<StructureSection load='5i4c' size='340' side='right' caption='[[5i4c]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5i4c' size='340' side='right'caption='[[5i4c]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i4c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5I4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I4C FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~rcsB~~, ~~b2217, JW2205 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83333 ECOLI])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5i4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4c OCA], [~~http~~://pdbe.org/5i4c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5i4c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5i4c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4c OCA], [https://pdbe.org/5i4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i4c RCSB], [https://www.ebi.ac.uk/pdbsum/5i4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RCSB_ECOLI RCSB_ECOLI]] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981]<ref>PMID:10702265</ref> <ref>PMID:11309126</ref> <ref>PMID:11566985</ref> <ref>PMID:13129944</ref> <ref>PMID:1597415</ref> <ref>PMID:20189963</ref> <ref>PMID:20952573</ref>	+	[https://www.uniprot.org/uniprot/RCSB_ECOLI RCSB_ECOLI] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981]<ref>PMID:10702265</ref> <ref>PMID:11309126</ref> <ref>PMID:11566985</ref> <ref>PMID:13129944</ref> <ref>PMID:1597415</ref> <ref>PMID:20189963</ref> <ref>PMID:20952573</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5i4c" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5i4c" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Response regulator 3D structure\|Response regulator 3D structure]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
-	[[Category: ~~Anderson, W F~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Structural genomic~~]]	+	[[Category: Anderson WF]]
-	[[Category: Filippova~~, E V~~]]	+	[[Category: Filippova EV]]
-	[[Category: Minasov, G]]	+	[[Category: Minasov G]]
-	[[Category: Pshenychnyi, S]]	+	[[Category: Pshenychnyi S]]
-	[[Category: Ruan, J]]	+	[[Category: Ruan J]]
-	[[Category: Wawrzak, Z]]	+	[[Category: Wawrzak Z]]
-	[[Category: Wolfe~~, A J]]~~	+	[[Category: Wolfe AJ]]
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Gene regulation]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Rcs phosphorelay]]~~	+
-	~~[[Category: Rpra]]~~	+
-	~~[[Category: Transcriptional regulator~~]]	+

OCA at 06:02, 1 November 2017

OCA — Wed, 01 Nov 2017 06:02:54 GMT

←Older revision		Revision as of 06:02, 1 November 2017
Line 3:		Line 3:
	<StructureSection load='5i4c' size='340' side='right' caption='[[5i4c]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5i4c' size='340' side='right' caption='[[5i4c]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i4c]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I4C FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4c OCA], [http://pdbe.org/5i4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i4c RCSB], [http://www.ebi.ac.uk/pdbsum/5i4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4c ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rcsB, b2217, JW2205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4c OCA], [http://pdbe.org/5i4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i4c RCSB], [http://www.ebi.ac.uk/pdbsum/5i4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 17:41, 19 October 2016

OCA — Wed, 19 Oct 2016 17:41:14 GMT

←Older revision		Revision as of 17:41, 19 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5i4c is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of non-phosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli==
		+	<StructureSection load='5i4c' size='340' side='right' caption='[[5i4c]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5i4c]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I4C FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4c OCA], [http://pdbe.org/5i4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i4c RCSB], [http://www.ebi.ac.uk/pdbsum/5i4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4c ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RCSB_ECOLI RCSB_ECOLI]] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981]<ref>PMID:10702265</ref> <ref>PMID:11309126</ref> <ref>PMID:11566985</ref> <ref>PMID:13129944</ref> <ref>PMID:1597415</ref> <ref>PMID:20189963</ref> <ref>PMID:20952573</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RcsB, the transcription-associated response regulator of the Rcs phosphorelay two-component signal transduction system, activates cell stress responses associated with desiccation, cell wall biosynthesis, cell division, virulence, biofilm formation and antibiotic resistance in enteric bacterial pathogens. RcsB belongs to the FixJ/NarL family of transcriptional regulators, which are characterized by a highly conserved C-terminal DNA-binding domain. The N-terminal domain of RcsB belongs to the family of two-component receiver domains. This receiver domain contains the phosphoacceptor site and participates in RcsB dimer formation; it also contributes to dimer formation with other transcription factor partners. Here, we describe the crystal structure of the Escherichia coli RcsB receiver domain in its non-phosphorylated state. The structure reveals important molecular details of phosphorylation-independent dimerization of RcsB and has implication for the formation of heterodimers. This article is protected by copyright. All rights reserved.

-	~~Authors:~~ Filippova~~, E.V.~~, Wawrzak, Z~~., Minasov, G.~~, Ruan, J., Pshenychnyi, S., Wolfe, A.J.~~, Anderson, W~~.F.~~, Center for Structural Genomics of Infectious Diseases (CSGID)~~	+	Crystal structure of non-phosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli.,Filippova EV, Wawrzak Z, Ruan J, Pshenychnyi S, Schultz RM, Wolfe AJ, Anderson WF Protein Sci. 2016 Sep 27. doi: 10.1002/pro.3050. PMID:27670836<ref>PMID:27670836</ref>

-	~~Description: Crystal structure of non-phosphorylated receiver domain~~ of the ~~stress response regulator RcsB from Escherichia coli~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Wolfe, A.J~~]]	+	<div class="pdbe-citations 5i4c" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Wawrzak~~, Z]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Anderson, W F]]
		+	[[Category: Structural genomic]]
		+	[[Category: Filippova, E V]]
	[[Category: Minasov, G]]		[[Category: Minasov, G]]
-	[[Category: Filippova, E.V]]
-	[[Category: Anderson, W.F]]
-	[[Category: Ruan, J]]
-	[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
	[[Category: Pshenychnyi, S]]		[[Category: Pshenychnyi, S]]
		+	[[Category: Ruan, J]]
		+	[[Category: Wawrzak, Z]]
		+	[[Category: Wolfe, A J]]
		+	[[Category: Csgid]]
		+	[[Category: Dna-binding]]
		+	[[Category: Gene regulation]]
		+	[[Category: Hydrolase]]
		+	[[Category: Rcs phosphorelay]]
		+	[[Category: Rpra]]
		+	[[Category: Transcriptional regulator]]

OCA at 01:51, 10 March 2016

OCA — Thu, 10 Mar 2016 01:51:11 GMT

←Older revision		Revision as of 01:51, 10 March 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5i4c is ON HOLD	+	The entry 5i4c is ON HOLD until Paper Publication

	Authors: Filippova, E.V., Wawrzak, Z., Minasov, G., Ruan, J., Pshenychnyi, S., Wolfe, A.J., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)		Authors: Filippova, E.V., Wawrzak, Z., Minasov, G., Ruan, J., Pshenychnyi, S., Wolfe, A.J., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

OCA: Protected "5i4c" [edit=sysop:move=sysop]

OCA — Sun, 21 Feb 2016 01:35:27 GMT

Protected "5i4c" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:35, 21 February 2016

OCA: New page: '''Unreleased structure''' The entry 5i4c is ON HOLD Authors: Filippova, E.V., Wawrzak, Z., Minasov, G., Ruan, J., Pshenychnyi, S., Wolfe, A.J., Anderson, W.F., Center for Structural Ge...

OCA — Sun, 21 Feb 2016 01:35:25 GMT

New page: '''Unreleased structure''' The entry 5i4c is ON HOLD Authors: Filippova, E.V., Wawrzak, Z., Minasov, G., Ruan, J., Pshenychnyi, S., Wolfe, A.J., Anderson, W.F., Center for Structural Ge...

New page

'''Unreleased structure'''

The entry 5i4c is ON HOLD

Authors: Filippova, E.V., Wawrzak, Z., Minasov, G., Ruan, J., Pshenychnyi, S., Wolfe, A.J., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: Crystal structure of non-phosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli
[[Category: Unreleased Structures]]
[[Category: Wolfe, A.J]]
[[Category: Wawrzak, Z]]
[[Category: Minasov, G]]
[[Category: Filippova, E.V]]
[[Category: Anderson, W.F]]
[[Category: Ruan, J]]
[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
[[Category: Pshenychnyi, S]]