5i79 - Revision history

OCA at 08:25, 23 August 2023

OCA — Wed, 23 Aug 2023 08:25:40 GMT

←Older revision		Revision as of 08:25, 23 August 2023
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	<StructureSection load='5i79' size='340' side='right'caption='[[5i79]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='5i79' size='340' side='right'caption='[[5i79]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i79]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~A._niger A.~~ niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I79 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5I79 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i79]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I79 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:~~CALCIUM+ION~~'>CA</scene>, <scene name='pdbligand=~~CTT~~:~~BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE~~'>~~CTT~~</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat">[[5i78\|5i78]], [[5i77\|5i77]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">eg1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 A. niger])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i79 OCA], [https://pdbe.org/5i79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i79 RCSB], [https://www.ebi.ac.uk/pdbsum/5i79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i79 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5i79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i79 OCA], [~~http~~://pdbe.org/5i79 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5i79 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5i79 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5i79 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/EGLB_ASPNG EGLB_ASPNG] Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates.<ref>PMID:16233124</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: A. niger]]	+	[[Category: Aspergillus niger]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen~~, C C~~]]	+	[[Category: Chen CC]]
-	[[Category: Guo~~, R T~~]]	+	[[Category: Guo RT]]
-	[[Category: Li~~, Y J~~]]	+	[[Category: Li YJ]]
-	[[Category: Liu~~, W D~~]]	+	[[Category: Liu WD]]
-	[[Category: Yan~~, J J~~]]	+	[[Category: Yan JJ]]
-	[[Category: Zheng~~, Y Y]]~~	+	[[Category: Zheng YY]]
-	~~[[Category: Endoglucanase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Substrate binding~~]]	+

OCA at 07:07, 3 June 2020

OCA — Wed, 03 Jun 2020 07:07:08 GMT

←Older revision		Revision as of 07:07, 3 June 2020
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	==Crystal structure of a beta-1,4-endoglucanase mutant from Aspergillus niger in complex with sugar==		==Crystal structure of a beta-1,4-endoglucanase mutant from Aspergillus niger in complex with sugar==
-	<StructureSection load='5i79' size='340' side='right' caption='[[5i79]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='5i79' size='340' side='right'caption='[[5i79]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i79]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I79 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5I79 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i79]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/A._niger A. niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I79 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5I79 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i78\|5i78]], [[5i77\|5i77]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i78\|5i78]], [[5i77\|5i77]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5i79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i79 OCA], [http://pdbe.org/5i79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i79 RCSB], [http://www.ebi.ac.uk/pdbsum/5i79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i79 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eg1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 A. niger])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5i79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i79 OCA], [http://pdbe.org/5i79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i79 RCSB], [http://www.ebi.ac.uk/pdbsum/5i79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i79 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5i79" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5i79" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: A. niger]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Guo, R T]]		[[Category: Guo, R T]]

OCA at 21:25, 22 December 2016

OCA — Thu, 22 Dec 2016 21:25:01 GMT

←Older revision		Revision as of 21:25, 22 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5i79 is ~~ON HOLD until Feb 17 2018~~	+	==Crystal structure of a beta-1,4-endoglucanase mutant from Aspergillus niger in complex with sugar==
		+	<StructureSection load='5i79' size='340' side='right' caption='[[5i79]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5i79]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I79 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I79 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i78\|5i78]], [[5i77\|5i77]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i79 OCA], [http://pdbe.org/5i79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i79 RCSB], [http://www.ebi.ac.uk/pdbsum/5i79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i79 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Eukaryotic 1,4-beta-endoglucanases (EC 3.2.1.4) have shown great potentials in many commercial applications because they effectively catalyze hydrolysis of cellulose, the main component of the plant cell wall. Here we expressed a glycoside hydrolase family (GH) 5 1,4-beta-endoglucanase from Aspergillus niger (AnCel5A) in Pichia pastoris, which exhibits outstanding pH and heat stability. In order to further investigate the molecular mechanism of AnCel5A, apo-form and cellotetraose (CTT) complex enzyme crystal structures were solved to high resolution. AnCel5A folds into a typical (beta/alpha)8-TIM barrel architecture, resembling other GH5 members. In the substrate binding cavity, CTT is found to bind to -4 - -1 subsites, and several polyethylene glycol molecules are found in positive subsites. In addition, several unique N-glycosylation motifs that may contribute to protein higher stability were observed from crystal structures. These results are of great importance for understanding the molecular mechanism of AnCel5A, and also provide guidance for further applications of the enzyme.

-	~~Authors: Liu~~, ~~W.D~~., Yan, J~~.J.~~, Li, Y~~.J.~~, Zheng, Y~~.Y.~~, Chen, C.C., ~~Guo, R~~.T.	+	Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase.,Yan J, Liu W, Li Y, Lai HL, Zheng Y, Huang JW, Chen CC, Chen Y, Jin J, Li H, Guo RT Biochem Biophys Res Commun. 2016 Jun 17;475(1):8-12. doi:, 10.1016/j.bbrc.2016.05.012. Epub 2016 May 3. PMID:27154222<ref>PMID:27154222</ref>

-	~~Description: Crystal structure of~~ a ~~beta~~-~~1,4~~-~~endoglucanase mutant from Aspergillus niger in complex with sugar~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Li, Y.J]]	+	<div class="pdbe-citations 5i79" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Chen~~, ~~C.C~~]]	+	== References ==
-	[[Category: Yan, J.J]]	+	<references/>
-	[[Category: ~~Liu~~, ~~W.D~~]]	+	__TOC__
-	[[Category: ~~Guo, R.T~~]]	+	</StructureSection>
-	[[Category: ~~Zheng, Y.Y~~]]	+	[[Category: Chen, C C]]
		+	[[Category: Guo, R T]]
		+	[[Category: Li, Y J]]
		+	[[Category: Liu, W D]]
		+	[[Category: Yan, J J]]
		+	[[Category: Zheng, Y Y]]
		+	[[Category: Endoglucanase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Substrate binding]]

OCA: Protected "5i79" [edit=sysop:move=sysop]

OCA — Fri, 26 Feb 2016 16:55:28 GMT

Protected "5i79" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:55, 26 February 2016

OCA: New page: '''Unreleased structure''' The entry 5i79 is ON HOLD until Feb 17 2018 Authors: Liu, W.D., Yan, J.J., Li, Y.J., Zheng, Y.Y., Chen, C.C., Guo, R.T. Description: Crystal structure of a b...

OCA — Fri, 26 Feb 2016 16:55:26 GMT

New page: '''Unreleased structure''' The entry 5i79 is ON HOLD until Feb 17 2018 Authors: Liu, W.D., Yan, J.J., Li, Y.J., Zheng, Y.Y., Chen, C.C., Guo, R.T. Description: Crystal structure of a b...

New page

'''Unreleased structure'''

The entry 5i79 is ON HOLD until Feb 17 2018

Authors: Liu, W.D., Yan, J.J., Li, Y.J., Zheng, Y.Y., Chen, C.C., Guo, R.T.

Description: Crystal structure of a beta-1,4-endoglucanase mutant from Aspergillus niger in complex with sugar
[[Category: Unreleased Structures]]
[[Category: Li, Y.J]]
[[Category: Chen, C.C]]
[[Category: Yan, J.J]]
[[Category: Liu, W.D]]
[[Category: Guo, R.T]]
[[Category: Zheng, Y.Y]]