5i82 - Revision history

OCA at 08:26, 23 August 2023

OCA — Wed, 23 Aug 2023 08:26:17 GMT

←Older revision		Revision as of 08:26, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='5i82' size='340' side='right'caption='[[5i82]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='5i82' size='340' side='right'caption='[[5i82]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i82]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_diphtheriae"_kruse_in_flugge_1886 "bacillus~~ diphtheriae~~" kruse in flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I82 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5I82 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i82]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I82 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[4ae0\|4ae0]]~~, ~~[[4ae1\|4ae1]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5i82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i82 OCA], [~~http~~://pdbe.org/5i82 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5i82 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5i82 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5i82 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i82 OCA], [https://pdbe.org/5i82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i82 RCSB], [https://www.ebi.ac.uk/pdbsum/5i82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i82 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6NK15_CORDI Q6NK15_CORDI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	It is established that the immunogenicity of polysaccharides is enhanced by coupling them to carrier proteins. Cross reacting material (CRM(197)), a nontoxic variant of diphtheria toxin (DT) is widely used carrier protein for polysaccharide conjugate vaccines. Conventionally, CRM(197) is isolated by fermentation of Corynebacterium diphtheriae C7 (beta(197)) cultures, which often suffers from low yield. Recently, several recombinant approaches have been reported with robust processes and higher yields, which will improve the affordability of CRM(197)-based vaccines. Vaccine manufacturers require detailed analytical information to ensure that the CRM(197) meets quality standards and regulatory requirements. In the present manuscript we have described detailed structural characteristics of Escherichia coli based recombinant CRM(197) (rCRM(197)) carrier protein. The crystal structure of the E. coli based rCRM(197) was found to be identical with the reported crystal structure of the C7 CRM(197) produced in C. diphtheriae C7 strain (Protein Data Bank (PDB) ID: 4EA0)(.) The crystal structure of rCRM(197) was determined at 2.3 A resolution and structure was submitted to the PDB with accession number ID 5I82. This is the first report of a crystal structure of E. coli derived recombinant CRM(197) carrier protein. Furthermore, the rCRM(197) was conjugated to Vi polysaccharide to generate Typhoid conjugate vaccine (Vi-rCRM(197)) and its immunogenicity was evaluated in Balb/C Mice. The Vi-rCRM(197) conjugate vaccine was found to generate strong primary alpha-Vi antibody response and also showed a booster response after subsequent vaccination in mice. Overall data suggest that E. coli based recombinant CRM(197) exhibits structural and immunological similarity with the C7 CRM(197) and can be used as a carrier protein in conjugate vaccine development.
		+
		+	Structural and immunological characterization of E. coli derived recombinant CRM(197) protein used as carrier in conjugate vaccines.,Mishra RPN, Yadav RSP, Jones C, Nocadello S, Minasov G, Shuvalova LA, Anderson WF, Goel A Biosci Rep. 2018 Sep 25;38(5):BSR20180238. doi: 10.1042/BSR20180238. Print 2018 , Oct 31. PMID:29875175<ref>PMID:29875175</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5i82" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Diphtheria toxin\|Diphtheria toxin]]		*[[Diphtheria toxin\|Diphtheria toxin]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ diphtheriae ~~kruse in flugge 1886~~]]	+	[[Category: Corynebacterium diphtheriae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Anderson~~, W F]]~~	+	[[Category: Anderson WF]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Dubrovska I]]
-	[[Category: Dubrovska, I]]	+	[[Category: Goel A]]
-	[[Category: Goel, A]]	+	[[Category: Minasov G]]
-	[[Category: Minasov, G]]	+	[[Category: Mishra RPN]]
-	[[Category: Mishra~~, R P.N~~]]	+	[[Category: Shuvalova L]]
-	[[Category: Shuvalova, L]]	+
-	~~[[Category: Crm197]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Diphtheria toxin]]~~	+
-	~~[[Category: Recombinant]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:08, 3 June 2020

OCA — Wed, 03 Jun 2020 07:08:22 GMT

←Older revision		Revision as of 07:08, 3 June 2020
Line 1:		Line 1:

	==First Crystal Structure of E.coli Based Recombinant Diphtheria Toxin Mutant CRM197==		==First Crystal Structure of E.coli Based Recombinant Diphtheria Toxin Mutant CRM197==
-	<StructureSection load='5i82' size='340' side='right' caption='[[5i82]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='5i82' size='340' side='right'caption='[[5i82]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i82]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I82 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5I82 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i82]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_diphtheriae"_kruse_in_flugge_1886 "bacillus diphtheriae" kruse in flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I82 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5I82 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ae0\|4ae0]], [[4ae1\|4ae1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ae0\|4ae0]], [[4ae1\|4ae1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5i82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i82 OCA], [http://pdbe.org/5i82 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i82 RCSB], [http://www.ebi.ac.uk/pdbsum/5i82 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5i82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i82 OCA], [http://pdbe.org/5i82 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i82 RCSB], [http://www.ebi.ac.uk/pdbsum/5i82 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i82 ProSAT]</span></td></tr>
	</table>		</table>
		+
		+	==See Also==
		+	*[[Diphtheria toxin\|Diphtheria toxin]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus diphtheriae kruse in flugge 1886]]
		+	[[Category: Large Structures]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 15:31, 2 March 2016

OCA — Wed, 02 Mar 2016 15:31:21 GMT

←Older revision		Revision as of 15:31, 2 March 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5i82 ~~is ON HOLD~~	+	==First Crystal Structure of E.coli Based Recombinant Diphtheria Toxin Mutant CRM197==
-		+	<StructureSection load='5i82' size='340' side='right' caption='[[5i82]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~Authors~~: ~~Minasov, G~~.~~, Shuvalova, L~~., ~~Mishra~~, R.P.N., ~~Goel, A~~.~~, Dubrovska, I~~., ~~Anderson~~, W.F.~~, Center for Structural Genomics of Infectious Diseases~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5i82]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I82 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I82 FirstGlance]. <br>
-	~~Description~~: ~~First Crystal Structure of E~~.~~coli Based Recombinant Diphtheria Toxin Mutant CRM197~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ae0\|4ae0]], [[4ae1\|4ae1]]</td></tr>
-	[[Category: ~~Mishra, R.P.N~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i82 OCA], [http://pdbe.org/5i82 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i82 RCSB], [http://www.ebi.ac.uk/pdbsum/5i82 PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Anderson, W F]]
		+	[[Category: Structural genomic]]
	[[Category: Dubrovska, I]]		[[Category: Dubrovska, I]]
-	[[Category: Center For Structural Genomics Of Infectious Diseases]]
	[[Category: Goel, A]]		[[Category: Goel, A]]
	[[Category: Minasov, G]]		[[Category: Minasov, G]]
-	[[Category: ~~Anderson~~, W.F]]	+	[[Category: Mishra, R P.N]]
	[[Category: Shuvalova, L]]		[[Category: Shuvalova, L]]
		+	[[Category: Crm197]]
		+	[[Category: Csgid]]
		+	[[Category: Diphtheria toxin]]
		+	[[Category: Recombinant]]
		+	[[Category: Transferase]]

OCA: Protected "5i82" [edit=sysop:move=sysop]

OCA — Fri, 26 Feb 2016 16:57:51 GMT

Protected "5i82" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:57, 26 February 2016

OCA: New page: '''Unreleased structure''' The entry 5i82 is ON HOLD Authors: Minasov, G., Shuvalova, L., Mishra, R.P.N., Goel, A., Dubrovska, I., Anderson, W.F., Center for Structural Genomics of Infe...

OCA — Fri, 26 Feb 2016 16:57:49 GMT

New page: '''Unreleased structure''' The entry 5i82 is ON HOLD Authors: Minasov, G., Shuvalova, L., Mishra, R.P.N., Goel, A., Dubrovska, I., Anderson, W.F., Center for Structural Genomics of Infe...

New page

'''Unreleased structure'''

The entry 5i82 is ON HOLD

Authors: Minasov, G., Shuvalova, L., Mishra, R.P.N., Goel, A., Dubrovska, I., Anderson, W.F., Center for Structural Genomics of Infectious Diseases.

Description: First Crystal Structure of E.coli Based Recombinant Diphtheria Toxin Mutant CRM197.
[[Category: Unreleased Structures]]
[[Category: Mishra, R.P.N]]
[[Category: Dubrovska, I]]
[[Category: Center For Structural Genomics Of Infectious Diseases]]
[[Category: Goel, A]]
[[Category: Minasov, G]]
[[Category: Anderson, W.F]]
[[Category: Shuvalova, L]]