5ia8 - Revision history

OCA at 13:43, 30 August 2023

OCA — Wed, 30 Aug 2023 13:43:28 GMT

←Older revision		Revision as of 13:43, 30 August 2023
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	<StructureSection load='5ia8' size='340' side='right'caption='[[5ia8]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5ia8' size='340' side='right'caption='[[5ia8]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ia8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IA8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5IA8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ia8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IA8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ia8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ia8 OCA], [~~http~~://pdbe.org/5ia8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ia8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ia8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ia8 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ia8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ia8 OCA], [https://pdbe.org/5ia8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ia8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ia8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ia8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/UBA5_HUMAN UBA5_HUMAN] E1-like enzyme which activates UFM1 and SUMO2.<ref>PMID:15071506</ref> <ref>PMID:18442052</ref> <ref>PMID:20368332</ref> [https://www.uniprot.org/uniprot/UFM1_HUMAN UFM1_HUMAN] Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.<ref>PMID:15071506</ref> <ref>PMID:20018847</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Ubiquitin activating enzyme\|Ubiquitin activating enzyme]]	+	*[[3D structures of Ubiquitin activating enzyme\|3D structures of Ubiquitin activating enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Oweis, W]]	+	[[Category: Oweis W]]
-	[[Category: Padala, P]]	+	[[Category: Padala P]]
-	[[Category: Wiener, R]]	+	[[Category: Wiener R]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Ubiquitin like protein~~]]	+

OCA at 07:09, 3 June 2020

OCA — Wed, 03 Jun 2020 07:09:45 GMT

←Older revision		Revision as of 07:09, 3 June 2020
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	==Structure of a Ubiquitin like protein with an E1 fragment==		==Structure of a Ubiquitin like protein with an E1 fragment==
-	<StructureSection load='5ia8' size='340' side='right' caption='[[5ia8]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5ia8' size='340' side='right'caption='[[5ia8]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ia8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IA8 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IA8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ia8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IA8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IA8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ia8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ia8 OCA], [http://pdbe.org/5ia8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ia8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ia8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ia8 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ia8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ia8 OCA], [http://pdbe.org/5ia8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ia8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ia8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ia8 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5ia8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ia8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ubiquitin activating enzyme\|Ubiquitin activating enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Oweis, W]]		[[Category: Oweis, W]]
	[[Category: Padala, P]]		[[Category: Padala, P]]

OCA at 05:52, 17 August 2017

OCA — Thu, 17 Aug 2017 05:52:51 GMT

←Older revision		Revision as of 05:52, 17 August 2017
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ia8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ia8 OCA], [http://pdbe.org/5ia8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ia8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ia8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ia8 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ia8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ia8 OCA], [http://pdbe.org/5ia8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ia8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ia8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ia8 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The modification of proteins by ubiquitin-fold modifier 1 (UFM1) is implicated in many human diseases. Prior to conjugation, UFM1 undergoes activation by its cognate activating enzyme, UBA5. UBA5 is a non-canonical E1 activating enzyme that possesses an adenylation domain but lacks a distinct cysteine domain. Binding of UBA5 to UFM1 is mediated via an amino acid sequence, known as the UFM1-interacting sequence (UIS), located outside the adenylation domain that is required for UFM1 activation. However, the precise boundaries of the UIS are yet not clear and are still under debate. Here we revisit the interaction of UFM1 with UBA5 by determining the crystal structure of UFM1 fused to 13 amino acids of human UBA5. Using binding and activity assays, we found that His 336 of UBA5, previously not reported to be part of the UIS, occupies a negatively charged pocket on UFM1's surface. This His is involved in UFM1 binding and if mutated perturbs activation of UFM1. Surprisingly, we also found that the interaction between two UFM1 molecules mimics how the UIS binds UFM1. Specifically, UFM1 His 70 resembles UBA5 His336 and enters a negatively charged pocked on the other UFM1 molecule. Our results refine our understanding of UFM1-UBA5 binding.
		+
		+	Novel insights into the interaction of UBA5 with UFM1 via a UFM1-interacting sequence.,Padala P, Oweis W, Mashahreh B, Soudah N, Cohen-Kfir E, Todd EA, Berndsen CE, Wiener R Sci Rep. 2017 Mar 30;7(1):508. doi: 10.1038/s41598-017-00610-0. PMID:28360427<ref>PMID:28360427</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ia8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 19:13, 11 March 2017

OCA — Sat, 11 Mar 2017 19:13:32 GMT

←Older revision		Revision as of 19:13, 11 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5ia8 ~~is ON HOLD until Paper Publication~~	+	==Structure of a Ubiquitin like protein with an E1 fragment==
-		+	<StructureSection load='5ia8' size='340' side='right' caption='[[5ia8]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~: ~~Oweis~~, W., ~~Padala~~, P., ~~Wiener~~, R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ia8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IA8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IA8 FirstGlance]. <br>
-	~~Description: Structure of a Ubiquitin like protein with an E1 fragment~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ia8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ia8 OCA], [http://pdbe.org/5ia8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ia8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ia8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ia8 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Oweis, W]]
	[[Category: Padala, P]]		[[Category: Padala, P]]
-	[[Category: Oweis, W]]
	[[Category: Wiener, R]]		[[Category: Wiener, R]]
		+	[[Category: Cell cycle]]
		+	[[Category: Ubiquitin like protein]]

OCA at 00:20, 11 May 2016

OCA — Wed, 11 May 2016 00:20:04 GMT

←Older revision		Revision as of 00:20, 11 May 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ia8 is ON HOLD	+	The entry 5ia8 is ON HOLD until Paper Publication

	Authors: Oweis, W., Padala, P., Wiener, R.		Authors: Oweis, W., Padala, P., Wiener, R.

OCA: Protected "5ia8" [edit=sysop:move=sysop]

OCA — Wed, 02 Mar 2016 13:37:30 GMT

Protected "5ia8" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:37, 2 March 2016

OCA: New page: '''Unreleased structure''' The entry 5ia8 is ON HOLD Authors: Oweis, W., Padala, P., Wiener, R. Description: Structure of a Ubiquitin like protein with an E1 fragment [[Category: Unrel...

OCA — Wed, 02 Mar 2016 13:37:28 GMT

New page: '''Unreleased structure''' The entry 5ia8 is ON HOLD Authors: Oweis, W., Padala, P., Wiener, R. Description: Structure of a Ubiquitin like protein with an E1 fragment [[Category: Unrel...

New page

'''Unreleased structure'''

The entry 5ia8 is ON HOLD

Authors: Oweis, W., Padala, P., Wiener, R.

Description: Structure of a Ubiquitin like protein with an E1 fragment
[[Category: Unreleased Structures]]
[[Category: Padala, P]]
[[Category: Oweis, W]]
[[Category: Wiener, R]]