5igo - Revision history

OCA at 13:51, 30 August 2023

OCA — Wed, 30 Aug 2023 13:51:58 GMT

←Older revision		Revision as of 13:51, 30 August 2023
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	==WD40 domain of Arabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from Trib1==		==WD40 domain of Arabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from Trib1==
-	<StructureSection load='5igo' size='340' side='right' caption='[[5igo]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='5igo' size='340' side='right'caption='[[5igo]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5igo]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IGO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5igo]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IGO FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~COP1~~, ~~At2g32950, T21L14.11 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=3702 ARATH])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igo OCA], [~~http~~://pdbe.org/5igo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5igo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5igo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5igo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igo OCA], [https://pdbe.org/5igo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5igo RCSB], [https://www.ebi.ac.uk/pdbsum/5igo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5igo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/COP1_ARATH COP1_ARATH]] E3 ubiquitin-protein ligase that acts as a repressor of photomorphogenesis and as an activator of etiolation in darkness. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Represses photomorphogenesis in darkness by mediating ubiquitination and subsequent proteasomal degradation of light-induced transcription factors such as HY5, HYH and LAF1. Down-regulates MYB21, probably via ubiquitination process. Light stimuli abrogate the repression of photomorphogenesis, possibly due to its localization to the cytoplasm. Could play a role in switching between skotomorphogenetic and photomorphogenetic pathways.<ref>PMID:11967090</ref> <ref>PMID:12023303</ref> [[http://www.uniprot.org/uniprot/TRIB1_HUMAN TRIB1_HUMAN]] Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.<ref>PMID:15299019</ref> <ref>PMID:15299019</ref>	+	[https://www.uniprot.org/uniprot/COP1_ARATH COP1_ARATH] E3 ubiquitin-protein ligase that acts as a repressor of photomorphogenesis and as an activator of etiolation in darkness. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Represses photomorphogenesis in darkness by mediating ubiquitination and subsequent proteasomal degradation of light-induced transcription factors such as HY5, HYH and LAF1. Down-regulates MYB21, probably via ubiquitination process. Light stimuli abrogate the repression of photomorphogenesis, possibly due to its localization to the cytoplasm. Could play a role in switching between skotomorphogenetic and photomorphogenetic pathways.<ref>PMID:11967090</ref> <ref>PMID:12023303</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5igo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5igo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ubiquitin protein ligase 3D structures\|Ubiquitin protein ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
-	[[Category: ~~Blacklow, S C~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Uljon, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hydrolase-peptide complex~~]]	+	[[Category: Blacklow SC]]
-	[[Category: ~~Wd40 domain e3 ligase~~]]	+	[[Category: Uljon S]]

OCA at 12:45, 16 November 2017

OCA — Thu, 16 Nov 2017 12:45:26 GMT

←Older revision		Revision as of 12:45, 16 November 2017
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	<StructureSection load='5igo' size='340' side='right' caption='[[5igo]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5igo' size='340' side='right' caption='[[5igo]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5igo]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5igo]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igo OCA], [http://pdbe.org/5igo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igo RCSB], [http://www.ebi.ac.uk/pdbsum/5igo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igo ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">COP1, At2g32950, T21L14.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igo OCA], [http://pdbe.org/5igo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igo RCSB], [http://www.ebi.ac.uk/pdbsum/5igo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Blacklow, S C]]		[[Category: Blacklow, S C]]
	[[Category: Uljon, S]]		[[Category: Uljon, S]]
	[[Category: Hydrolase-peptide complex]]		[[Category: Hydrolase-peptide complex]]
	[[Category: Wd40 domain e3 ligase]]		[[Category: Wd40 domain e3 ligase]]

OCA at 06:52, 26 July 2016

OCA — Tue, 26 Jul 2016 06:52:44 GMT

←Older revision		Revision as of 06:52, 26 July 2016
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5igo]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGO FirstGlance]. <br>		<table><tr><td colspan='2'>[[5igo]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igo OCA], [http://pdbe.org/5igo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igo RCSB], [http://www.ebi.ac.uk/pdbsum/5igo PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igo OCA], [http://pdbe.org/5igo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igo RCSB], [http://www.ebi.ac.uk/pdbsum/5igo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 05:49, 11 May 2016

OCA — Wed, 11 May 2016 05:49:17 GMT

←Older revision		Revision as of 05:49, 11 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5igo is ~~ON HOLD~~	+	==WD40 domain of Arabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from Trib1==
		+	<StructureSection load='5igo' size='340' side='right' caption='[[5igo]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5igo]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGO FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igo OCA], [http://pdbe.org/5igo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igo RCSB], [http://www.ebi.ac.uk/pdbsum/5igo PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/COP1_ARATH COP1_ARATH]] E3 ubiquitin-protein ligase that acts as a repressor of photomorphogenesis and as an activator of etiolation in darkness. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Represses photomorphogenesis in darkness by mediating ubiquitination and subsequent proteasomal degradation of light-induced transcription factors such as HY5, HYH and LAF1. Down-regulates MYB21, probably via ubiquitination process. Light stimuli abrogate the repression of photomorphogenesis, possibly due to its localization to the cytoplasm. Could play a role in switching between skotomorphogenetic and photomorphogenetic pathways.<ref>PMID:11967090</ref> <ref>PMID:12023303</ref> [[http://www.uniprot.org/uniprot/TRIB1_HUMAN TRIB1_HUMAN]] Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity.<ref>PMID:15299019</ref> <ref>PMID:15299019</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	COP1 proteins are E3 ubiquitin ligases that regulate phototropism in plants and target transcription factors for degradation in mammals. The substrate-binding region of COP1 resides within a WD40-repeat domain that also binds to Trib proteins, which are adaptors for C/EBPalpha degradation. Here we report structures of the human COP1 WD40 domain in isolation, and complexes of the human and Arabidopsis thaliana COP1 WD40 domains with the binding motif of Trib1. The human and Arabidopsis WD40 domains are seven-bladed beta propellers with an inserted loop on the bottom face of the first blade. The Trib1 peptide binds in an extended conformation to a highly conserved surface on the top face of the beta propeller, indicating a general mode for recognition of peptide motifs by COP1. Together, these studies identify the structural basis and key interactions for motif recognition by COP1, and hint at how Trib1 autoinhibition is overcome to target C/EBPalpha for degradation.

-	~~Authors: Uljon~~, S., ~~Blacklow~~, S.C.	+	Structural Basis for Substrate Selectivity of the E3 Ligase COP1.,Uljon S, Xu X, Durzynska I, Stein S, Adelmant G, Marto JA, Pear WS, Blacklow SC Structure. 2016 May 3;24(5):687-96. doi: 10.1016/j.str.2016.03.002. Epub 2016 Mar, 31. PMID:27041596<ref>PMID:27041596</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5igo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Blacklow, S C]]
	[[Category: Uljon, S]]		[[Category: Uljon, S]]
-	[[Category: ~~Blacklow, S.C~~]]	+	[[Category: Hydrolase-peptide complex]]
		+	[[Category: Wd40 domain e3 ligase]]

OCA: Protected "5igo" [edit=sysop:move=sysop]

OCA — Thu, 10 Mar 2016 02:17:22 GMT

Protected "5igo" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:17, 10 March 2016

OCA: New page: '''Unreleased structure''' The entry 5igo is ON HOLD Authors: Uljon, S., Blacklow, S.C. Description: Category: Unreleased Structures Category: Uljon, S [[Category: Blacklow, S...

OCA — Thu, 10 Mar 2016 02:17:21 GMT

New page: '''Unreleased structure''' The entry 5igo is ON HOLD Authors: Uljon, S., Blacklow, S.C. Description: Category: Unreleased Structures Category: Uljon, S [[Category: Blacklow, S...

New page

'''Unreleased structure'''

The entry 5igo is ON HOLD

Authors: Uljon, S., Blacklow, S.C.

Description:
[[Category: Unreleased Structures]]
[[Category: Uljon, S]]
[[Category: Blacklow, S.C]]