5ijt - Revision history

OCA at 13:58, 30 August 2023

OCA — Wed, 30 Aug 2023 13:58:03 GMT

←Older revision		Revision as of 13:58, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='5ijt' size='340' side='right'caption='[[5ijt]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5ijt' size='340' side='right'caption='[[5ijt]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ijt]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IJT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ijt]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IJT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.148Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">PRDX2, NKEFB, TDPX1 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ijt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ijt OCA], [https://pdbe.org/5ijt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ijt RCSB], [https://www.ebi.ac.uk/pdbsum/5ijt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ijt ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ijt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ijt OCA], [~~http~~://pdbe.org/5ijt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ijt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ijt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ijt ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PRDX2_HUMAN PRDX2_HUMAN]] Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).	+	[https://www.uniprot.org/uniprot/PRDX2_HUMAN PRDX2_HUMAN] Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Peroxiredoxin]]~~	+	[[Category: Bolduc JA]]
-	[[Category: Bolduc~~, J A~~]]	+	[[Category: Haynes AC]]
-	[[Category: Haynes~~, A C~~]]	+	[[Category: Lowther WT]]
-	[[Category: Lowther~~, W T]]~~	+
-	~~[[Category: Decamer disulfide bond]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 11:49, 25 December 2019

OCA — Wed, 25 Dec 2019 11:49:11 GMT

←Older revision		Revision as of 11:49, 25 December 2019
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Peroxiredoxin\|Peroxiredoxin]]	+	*[[Peroxiredoxin 3D structures\|Peroxiredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:53, 27 March 2019

OCA — Wed, 27 Mar 2019 06:53:21 GMT

←Older revision		Revision as of 06:53, 27 March 2019
Line 1:		Line 1:

	==Human Peroxiredoxin 2 Oxidized (SS)==		==Human Peroxiredoxin 2 Oxidized (SS)==
-	<StructureSection load='5ijt' size='340' side='right' caption='[[5ijt]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='5ijt' size='340' side='right'caption='[[5ijt]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ijt]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IJT FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ijt]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IJT FirstGlance]. <br>
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PRDX2_HUMAN PRDX2_HUMAN]] Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).		[[http://www.uniprot.org/uniprot/PRDX2_HUMAN PRDX2_HUMAN]] Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	2-Cys peroxiredoxins (Prxs) modulate hydrogen peroxide (H2O2)-mediated cell signaling. At high H2O2 levels, eukaryotic Prxs can be inactivated by hyperoxidation and are classified as sensitive Prxs. In contrast, prokaryotic Prxs are categorized as being resistant to hyperoxidation and lack the GGLG and C-terminal YF motifs present in the sensitive Prxs. Additional molecular determinants that account for the subtle differences in the susceptibility to hyperoxidation remain to be identified. A comparison of a new, 2.15-A-resolution crystal structure of Prx2 in the oxidized, disulfide-bonded state with the hyperoxidized structure of Prx2 and Prx1 in complex with sulfiredoxin revealed three structural regions that rearrange during catalysis. With these regions in hand, focused sequence analyses were performed comparing sensitive and resistant Prx groups. From this combinatorial approach, we discovered two novel hyperoxidation resistance motifs, motifs A and B, which were validated using mutagenesis of sensitive human Prxs and resistant Salmonella enterica serovar Typhimurium AhpC. Introduction and removal of these motifs, respectively, resulted in drastic changes in the sensitivity to hyperoxidation with Prx1 becoming 100-fold more resistant to hyperoxidation and AhpC becoming 800-fold more sensitive to hyperoxidation. The increased sensitivity of the latter AhpC variant was also confirmed in vivo These results support the function of motifs A and B as primary drivers for tuning the sensitivity of Prxs to different levels of H2O2, thus enabling the initiation of variable signaling or antioxidant responses in cells.
		+
		+	Novel hyperoxidation resistance motifs in 2-Cys peroxiredoxins.,Bolduc JA, Nelson KJ, Haynes AC, Lee J, Reisz JA, Graff AH, Clodfelter JE, Parsonage D, Poole LB, Furdui CM, Lowther WT J Biol Chem. 2018 Jul 27;293(30):11901-11912. doi: 10.1074/jbc.RA117.001690. Epub, 2018 Jun 8. PMID:29884768<ref>PMID:29884768</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ijt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Peroxiredoxin\|Peroxiredoxin]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Peroxiredoxin]]		[[Category: Peroxiredoxin]]
	[[Category: Bolduc, J A]]		[[Category: Bolduc, J A]]

OCA at 12:51, 6 November 2017

OCA — Mon, 06 Nov 2017 12:51:26 GMT

←Older revision		Revision as of 12:51, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5ijt' size='340' side='right' caption='[[5ijt]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5ijt' size='340' side='right' caption='[[5ijt]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ijt]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ijt]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IJT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRDX2, NKEFB, TDPX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ijt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ijt OCA], [http://pdbe.org/5ijt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ijt RCSB], [http://www.ebi.ac.uk/pdbsum/5ijt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ijt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ijt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ijt OCA], [http://pdbe.org/5ijt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ijt RCSB], [http://www.ebi.ac.uk/pdbsum/5ijt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ijt ProSAT]</span></td></tr>
Line 12:		Line 13:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Peroxiredoxin]]		[[Category: Peroxiredoxin]]
	[[Category: Bolduc, J A]]		[[Category: Bolduc, J A]]

OCA at 09:30, 13 September 2017

OCA — Wed, 13 Sep 2017 09:30:34 GMT

←Older revision		Revision as of 09:30, 13 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ijt is ~~ON HOLD until Paper Publication~~	+	==Human Peroxiredoxin 2 Oxidized (SS)==
-		+	<StructureSection load='5ijt' size='340' side='right' caption='[[5ijt]], [[Resolution\|resolution]] 2.15Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ijt]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IJT FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ijt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ijt OCA], [http://pdbe.org/5ijt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ijt RCSB], [http://www.ebi.ac.uk/pdbsum/5ijt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ijt ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRDX2_HUMAN PRDX2_HUMAN]] Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Peroxiredoxin]]
		+	[[Category: Bolduc, J A]]
		+	[[Category: Haynes, A C]]
		+	[[Category: Lowther, W T]]
		+	[[Category: Decamer disulfide bond]]
		+	[[Category: Oxidoreductase]]

OCA at 14:28, 15 May 2016

OCA — Sun, 15 May 2016 14:28:51 GMT

←Older revision		Revision as of 14:28, 15 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ijt is ON HOLD	+	The entry 5ijt is ON HOLD until Paper Publication

-	Authors: ~~Haynes, A.C., Bolduc, J.A., Lowther, W.T.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Bolduc, J.A]]
-	[[Category: Lowther, W.T]]
-	[[Category: Haynes, A.C]]

OCA at 00:33, 11 May 2016

OCA — Wed, 11 May 2016 00:33:29 GMT

←Older revision		Revision as of 00:33, 11 May 2016
Line 3:		Line 3:
	The entry 5ijt is ON HOLD		The entry 5ijt is ON HOLD

-	Authors: Haynes, AC, Bolduc, JA, Lowther, WT	+	Authors: Haynes, A.C., Bolduc, J.A., Lowther, W.T.

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Haynes, Ac,~~ Bolduc, Ja, ~~Lowther~~, Wt]]	+	[[Category: Bolduc, J.A]]
		+	[[Category: Lowther, W.T]]
		+	[[Category: Haynes, A.C]]

OCA: Protected "5ijt" [edit=sysop:move=sysop]

OCA — Thu, 10 Mar 2016 02:27:21 GMT

Protected "5ijt" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:27, 10 March 2016

OCA: New page: '''Unreleased structure''' The entry 5ijt is ON HOLD Authors: Haynes, AC, Bolduc, JA, Lowther, WT Description: Category: Unreleased Structures [[Category: Haynes, Ac, Bolduc, Ja, ...

OCA — Thu, 10 Mar 2016 02:27:20 GMT

New page: '''Unreleased structure''' The entry 5ijt is ON HOLD Authors: Haynes, AC, Bolduc, JA, Lowther, WT Description: Category: Unreleased Structures [[Category: Haynes, Ac, Bolduc, Ja, ...

New page

'''Unreleased structure'''

The entry 5ijt is ON HOLD

Authors: Haynes, AC, Bolduc, JA, Lowther, WT

Description:
[[Category: Unreleased Structures]]
[[Category: Haynes, Ac, Bolduc, Ja, Lowther, Wt]]