5ikx - Revision history

OCA at 13:59, 30 August 2023

OCA — Wed, 30 Aug 2023 13:59:15 GMT

←Older revision		Revision as of 13:59, 30 August 2023
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	<StructureSection load='5ikx' size='340' side='right'caption='[[5ikx]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='5ikx' size='340' side='right'caption='[[5ikx]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ikx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Australian_sheep_blowfly Australian sheep blowfly~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4fg5 4fg5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5IKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ikx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lucilia_cuprina Lucilia cuprina]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4fg5 4fg5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IKX FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~LcaE7 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=7375 Australian sheep blowfly])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.19Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ikx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikx OCA], [~~http~~://pdbe.org/5ikx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ikx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ikx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ikx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ikx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikx OCA], [https://pdbe.org/5ikx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ikx RCSB], [https://www.ebi.ac.uk/pdbsum/5ikx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ikx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q25252_LUCCU Q25252_LUCCU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Australian sheep blowfly]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Fraser, N~~]]	+	[[Category: Lucilia cuprina]]
-	[[Category: ~~Jackson, C~~]]	+	[[Category: Fraser N]]
-	[[Category: ~~Carboxylesterase]]~~	+	[[Category: Jackson C]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Oligermerization]]~~	+
-	~~[[Category: Organophosphate~~]]	+

OCA at 07:22, 3 June 2020

OCA — Wed, 03 Jun 2020 07:22:17 GMT

←Older revision		Revision as of 07:22, 3 June 2020
Line 1:		Line 1:

	==Crystal structure of the alpha-esterase-7 carboxyl esterase (dimer), E3, from Lucilia cuprina==		==Crystal structure of the alpha-esterase-7 carboxyl esterase (dimer), E3, from Lucilia cuprina==
-	<StructureSection load='5ikx' size='340' side='right' caption='[[5ikx]], [[Resolution\|resolution]] 2.19Å' scene=''>	+	<StructureSection load='5ikx' size='340' side='right'caption='[[5ikx]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ikx]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4fg5 4fg5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ikx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Australian_sheep_blowfly Australian sheep blowfly]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4fg5 4fg5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IKX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ikx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikx OCA], [http://pdbe.org/5ikx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ikx RCSB], [http://www.ebi.ac.uk/pdbsum/5ikx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ikx ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LcaE7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7375 Australian sheep blowfly])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ikx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikx OCA], [http://pdbe.org/5ikx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ikx RCSB], [http://www.ebi.ac.uk/pdbsum/5ikx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ikx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 5ikx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ikx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Carboxylesterase 3D structures\|Carboxylesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Australian sheep blowfly]]
		+	[[Category: Large Structures]]
	[[Category: Fraser, N]]		[[Category: Fraser, N]]
	[[Category: Jackson, C]]		[[Category: Jackson, C]]

OCA at 02:27, 13 July 2016

OCA — Wed, 13 Jul 2016 02:27:39 GMT

←Older revision		Revision as of 02:27, 13 July 2016
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ikx]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4fg5 4fg5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IKX FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ikx]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4fg5 4fg5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IKX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ikx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikx OCA], [http://pdbe.org/5ikx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ikx RCSB], [http://www.ebi.ac.uk/pdbsum/5ikx PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ikx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikx OCA], [http://pdbe.org/5ikx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ikx RCSB], [http://www.ebi.ac.uk/pdbsum/5ikx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ikx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:06, 23 June 2016

OCA — Thu, 23 Jun 2016 23:06:34 GMT

←Older revision		Revision as of 23:06, 23 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The~~ entry 5ikx is ~~ON HOLD until sometime~~ in the ~~future~~	+	==Crystal structure of the alpha-esterase-7 carboxyl esterase (dimer), E3, from Lucilia cuprina==
		+	<StructureSection load='5ikx' size='340' side='right' caption='[[5ikx]], [[Resolution\|resolution]] 2.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ikx]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4fg5 4fg5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IKX FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ikx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikx OCA], [http://pdbe.org/5ikx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ikx RCSB], [http://www.ebi.ac.uk/pdbsum/5ikx PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Oligomerization has been suggested to be an important mechanism for increasing or maintaining the thermostability of proteins. Although it is evident that protein-protein contacts can result in substantial stabilization in many extant proteins, evidence for evolutionary selection for oligomerization is largely indirect and little is understood of the early steps in the evolution of oligomers. A laboratory-directed evolution experiment that selected for increased thermostability in the alphaE7 carboxylesterase from the Australian sheep blowfly, Lucilia cuprina, resulted in a thermostable variant, LcalphaE7-4a, that displayed increased levels of dimeric and tetrameric quaternary structure. A trade-off between activity and thermostability was made during the evolution of thermostability, with the higher-order oligomeric species displaying the greatest thermostability and lowest catalytic activity. Analysis of monomeric and dimeric LcalphaE7-4a crystal structures revealed that only one of the oligomerization-inducing mutations was located at a potential protein-protein interface. This work demonstrates that by imposing a selective pressure demanding greater thermostability, mutations can lead to increased oligomerization and stabilization, providing support for the hypothesis that oligomerization is a viable evolutionary strategy for protein stabilization.

-	~~Authors~~:	+	Evolution of Protein Quaternary Structure in Response to Selective Pressure for Increased Thermostability.,Fraser NJ, Liu JW, Mabbitt PD, Correy GJ, Coppin CW, Lethier M, Perugini MA, Murphy JM, Oakeshott JG, Weik M, Jackson CJ J Mol Biol. 2016 Jun 5;428(11):2359-71. doi: 10.1016/j.jmb.2016.03.014. Epub 2016, Mar 22. PMID:27016206<ref>PMID:27016206</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5ikx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fraser, N]]
		+	[[Category: Jackson, C]]
		+	[[Category: Carboxylesterase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Oligermerization]]
		+	[[Category: Organophosphate]]

OCA at 14:30, 15 May 2016

OCA — Sun, 15 May 2016 14:30:39 GMT

←Older revision		Revision as of 14:30, 15 May 2016
Line 3:		Line 3:
	The entry 5ikx is ON HOLD until sometime in the future		The entry 5ikx is ON HOLD until sometime in the future

-	Authors: ~~Jackson, C., Fraser, N.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Jackson, C]]
-	[[Category: Fraser, N]]

OCA at 00:35, 11 May 2016

OCA — Wed, 11 May 2016 00:35:40 GMT

←Older revision		Revision as of 00:35, 11 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ikx is ON HOLD	+	The entry 5ikx is ON HOLD until sometime in the future

-	Authors: ~~Colin~~ Jackson, ~~Nicholas~~ Fraser	+	Authors: Jackson, C., Fraser, N.

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Colin~~ Jackson, ~~Nicholas~~ Fraser]]	+	[[Category: Jackson, C]]
		+	[[Category: Fraser, N]]

OCA: Protected "5ikx" [edit=sysop:move=sysop]

OCA — Thu, 10 Mar 2016 02:31:23 GMT

Protected "5ikx" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:31, 10 March 2016

OCA: New page: '''Unreleased structure''' The entry 5ikx is ON HOLD Authors: Colin Jackson, Nicholas Fraser Description: Category: Unreleased Structures [[Category: Colin Jackson, Nicholas Frase...

OCA — Thu, 10 Mar 2016 02:31:22 GMT

New page: '''Unreleased structure''' The entry 5ikx is ON HOLD Authors: Colin Jackson, Nicholas Fraser Description: Category: Unreleased Structures [[Category: Colin Jackson, Nicholas Frase...

New page

'''Unreleased structure'''

The entry 5ikx is ON HOLD

Authors: Colin Jackson, Nicholas Fraser

Description:
[[Category: Unreleased Structures]]
[[Category: Colin Jackson, Nicholas Fraser]]