5in3 - Revision history

OCA at 14:02, 30 August 2023

OCA — Wed, 30 Aug 2023 14:02:09 GMT

←Older revision		Revision as of 14:02, 30 August 2023
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	<StructureSection load='5in3' size='340' side='right'caption='[[5in3]], [[Resolution\|resolution]] 1.73Å' scene=''>		<StructureSection load='5in3' size='340' side='right'caption='[[5in3]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5in3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IN3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IN3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5in3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IN3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IN3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.73Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GALT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose--hexose-1-phosphate_uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.12 2.7.7.12] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5in3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5in3 OCA], [https://pdbe.org/5in3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5in3 RCSB], [https://www.ebi.ac.uk/pdbsum/5in3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5in3 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5in3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5in3 OCA], [~~http~~://pdbe.org/5in3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5in3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5in3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5in3 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/GALT_HUMAN GALT_HUMAN]] Classic galactosemia. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/GALT_HUMAN GALT_HUMAN] Classic galactosemia. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GALT_HUMAN GALT_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: UDP-glucose--hexose-1-phosphate uridylyltransferase]]~~	+	[[Category: Arrowsmith C]]
-	[[Category: Arrowsmith, C]]	+	[[Category: Bountra C]]
-	[[Category: Bountra, C]]	+	[[Category: Burgess-Brown N]]
-	[[Category: Burgess-Brown, N]]	+	[[Category: Chalk R]]
-	[[Category: Chalk, R]]	+	[[Category: Edwards A]]
-	~~[[Category: Delft, F von~~]]	+	[[Category: Fitzpatrick F]]
-	[[Category: Edwards, A]]	+	[[Category: Kopec J]]
-	[[Category: Fitzpatrick, F]]	+	[[Category: McCorvie T]]
-	[[Category: Kopec, J]]	+	[[Category: Patel D]]
-	[[Category: McCorvie, T]]	+	[[Category: Shrestha L]]
-	[[Category: Patel, D]]	+	[[Category: Tallant C]]
-	[[Category: Shrestha, L]]	+	[[Category: Velupillai S]]
-	[[Category: Tallant, C]]	+	[[Category: Yue WW]]
-	[[Category: Velupillai, S]]	+	[[Category: Von Delft F]]
-	[[Category: Yue~~, W W~~]]	+
-	[[Category: ~~Galactose-1-phosphate uridylyltransferase]]~~	+
-	~~[[Category: Galt]]~~	+
-	~~[[Category: Glucose-1-phosphate]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Ump-galt~~]]	+

OCA at 06:23, 16 October 2019

OCA — Wed, 16 Oct 2019 06:23:24 GMT

←Older revision		Revision as of 06:23, 16 October 2019
Line 1:		Line 1:

	==Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase==		==Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase==
-	<StructureSection load='5in3' size='340' side='right' caption='[[5in3]], [[Resolution\|resolution]] 1.73Å' scene=''>	+	<StructureSection load='5in3' size='340' side='right'caption='[[5in3]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5in3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IN3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IN3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5in3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IN3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IN3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GALT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose--hexose-1-phosphate_uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.12 2.7.7.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose--hexose-1-phosphate_uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.12 2.7.7.12] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5in3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5in3 OCA], [http://pdbe.org/5in3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5in3 RCSB], [http://www.ebi.ac.uk/pdbsum/5in3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5in3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5in3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5in3 OCA], [http://pdbe.org/5in3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5in3 RCSB], [http://www.ebi.ac.uk/pdbsum/5in3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5in3 ProSAT]</span></td></tr>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: UDP-glucose--hexose-1-phosphate uridylyltransferase]]		[[Category: UDP-glucose--hexose-1-phosphate uridylyltransferase]]
	[[Category: Arrowsmith, C]]		[[Category: Arrowsmith, C]]

OCA at 08:23, 14 September 2016

OCA — Wed, 14 Sep 2016 08:23:48 GMT

←Older revision		Revision as of 08:23, 14 September 2016
Line 6:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose--hexose-1-phosphate_uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.12 2.7.7.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose--hexose-1-phosphate_uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.12 2.7.7.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5in3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5in3 OCA], [http://pdbe.org/5in3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5in3 RCSB], [http://www.ebi.ac.uk/pdbsum/5in3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5in3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5in3 OCA], [http://pdbe.org/5in3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5in3 RCSB], [http://www.ebi.ac.uk/pdbsum/5in3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5in3 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/GALT_HUMAN GALT_HUMAN]] Classic galactosemia. The disease is caused by mutations affecting the gene represented in this entry.		[[http://www.uniprot.org/uniprot/GALT_HUMAN GALT_HUMAN]] Classic galactosemia. The disease is caused by mutations affecting the gene represented in this entry.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Classic galactosemia is a potentially lethal disease caused by the dysfunction of galactose 1-phosphate uridylyltransferase (GALT). Over 300 disease-associated GALT mutations have been reported, with the majority being missense changes, although a better understanding of their underlying molecular effects has been hindered by the lack of structural information for the human enzyme. Here, we present the 1.9 A resolution crystal structure of human GALT (hGALT) ternary complex, revealing a homodimer arrangement that contains a covalent uridylylated intermediate and glucose-1-phosphate in the active site, as well as a structural zinc-binding site, per monomer. hGALT reveals significant structural differences from bacterial GALT homologues in metal ligation and dimer interactions, and therefore is a zbetter model for understanding the molecular consequences of disease mutations. Both uridylylation and zinc binding influence the stability and aggregation tendency of hGALT. This has implications for disease-associated variants where p.Gln188Arg, the most commonly detected, increases the rate of aggregation in the absence of zinc likely due to its reduced ability to form the uridylylated intermediate. As such our structure serves as a template in the future design of pharmacological chaperone therapies and opens new concepts about the roles of metal binding and activity in protein misfolding by disease-associated mutants.
		+
		+	Molecular basis of classic galactosemia from the structure of human galactose 1-phosphate uridylyltransferase.,McCorvie TJ, Kopec J, Pey AL, Fitzpatrick F, Patel D, Chalk R, Shrestha L, Yue WW Hum Mol Genet. 2016 Mar 22. pii: ddw091. PMID:27005423<ref>PMID:27005423</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5in3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA: Protected "5in3" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 20:37:27 GMT

Protected "5in3" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:37, 11 May 2016

OCA: New page: ==Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase== == Structural highlights ==
<table><tr><td colspan='2'>[[5in3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IN3 OCA]. For a guided tour on the structure components use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IN3 FirstGlance]. 
</td></tr><tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='activity'><td class="sblockLbl">Activity:</td><td class="sblockDat">[http://en.wikipedia.org/wiki/UDP-glucose--hexose-1-phosphate_uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.12 2.7.7.12] </td></tr>
<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5in3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5in3 OCA], [http://pdbe.org/5in3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5in3 RCSB], [http://www.ebi.ac.uk/pdbsum/5in3 PDBsum]</td></tr>
</table>
== Disease ==
[[http://www.uniprot.org/uniprot/GALT_HUMAN GALT_HUMAN]] Classic galactosemia. The disease is caused by mutations affecting the gene represented in this entry.
TOC
</StructureSection>
[[Category: UDP-glucose--hexose-1-phosphate uridylyltransferase]]
[[Category: Arrowsmith, C]]
[[Category: Bountra, C]]
[[Category: Burgess-Brown, N]]
[[Category: Chalk, R]]
[[Category: Delft, F von]]
[[Category: Edwards, A]]
[[Category: Fitzpatrick, F]]
[[Category: Kopec, J]]
[[Category: McCorvie, T]]
[[Category: Patel, D]]
[[Category: Shrestha, L]]
[[Category: Tallant, C]]
[[Category: Velupillai, S]]
[[Category: Yue, W W]]
[[Category: Galactose-1-phosphate uridylyltransferase]]
[[Category: Galt]]
[[Category: Glucose-1-phosphate]]
[[Category: Transferase]]
[[Category: Ump-galt]]