5iqn - Revision history

OCA at 14:06, 30 August 2023

OCA — Wed, 30 Aug 2023 14:06:23 GMT

←Older revision		Revision as of 14:06, 30 August 2023
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	<StructureSection load='5iqn' size='340' side='right'caption='[[5iqn]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='5iqn' size='340' side='right'caption='[[5iqn]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5iqn]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IQN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5iqn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IQN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CO:COBALT+(II)+ION'>CO</scene~~>, ~~<scene name='pdbligand=EDO:~~1~~,2-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~fimG, b4319, JW4282 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5iqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iqn OCA], [~~http~~://pdbe.org/5iqn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5iqn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5iqn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5iqn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iqn OCA], [https://pdbe.org/5iqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iqn RCSB], [https://www.ebi.ac.uk/pdbsum/5iqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iqn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FIMG_ECOLI FIMG_ECOLI]] Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Involved in the integration of FimH in the fimbriae. [[http://www.uniprot.org/uniprot/FIMF_ECOLI FIMF_ECOLI]] Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Involved in the integration of FimH in the fimbriae.	+	[https://www.uniprot.org/uniprot/FIMG_ECOLI FIMG_ECOLI] Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Involved in the integration of FimH in the fimbriae.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Capitani, G]]	+	[[Category: Capitani G]]
-	[[Category: Eras, J]]	+	[[Category: Eras J]]
-	[[Category: Giese, C]]	+	[[Category: Giese C]]
-	[[Category: Glockshuber, R]]	+	[[Category: Glockshuber R]]
-	[[Category: Kern, A]]	+	[[Category: Kern A]]
-	[[Category: Scharer~~, M A]]~~	+	[[Category: Scharer MA]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Fimgt]]~~	+
-	~~[[Category: Protein~~]]	+

OCA at 16:17, 20 November 2019

OCA — Wed, 20 Nov 2019 16:17:54 GMT

←Older revision		Revision as of 16:17, 20 November 2019
Line 1:		Line 1:

	==Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_SRIRIRGYVR==		==Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_SRIRIRGYVR==
-	<StructureSection load='5iqn' size='340' side='right' caption='[[5iqn]], [[Resolution\|resolution]] 1.00Å' scene=''>	+	<StructureSection load='5iqn' size='340' side='right'caption='[[5iqn]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5iqn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5iqn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IQN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fimG, b4319, JW4282 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iqn OCA], [http://pdbe.org/5iqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iqn RCSB], [http://www.ebi.ac.uk/pdbsum/5iqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iqn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iqn OCA], [http://pdbe.org/5iqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iqn RCSB], [http://www.ebi.ac.uk/pdbsum/5iqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iqn ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Capitani, G]]		[[Category: Capitani, G]]
	[[Category: Eras, J]]		[[Category: Eras, J]]

OCA at 02:21, 13 July 2016

OCA — Wed, 13 Jul 2016 02:21:18 GMT

←Older revision		Revision as of 02:21, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5iqn is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_SRIRIRGYVR==
		+	<StructureSection load='5iqn' size='340' side='right' caption='[[5iqn]], [[Resolution\|resolution]] 1.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5iqn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IQN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iqn OCA], [http://pdbe.org/5iqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iqn RCSB], [http://www.ebi.ac.uk/pdbsum/5iqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iqn ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FIMG_ECOLI FIMG_ECOLI]] Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Involved in the integration of FimH in the fimbriae. [[http://www.uniprot.org/uniprot/FIMF_ECOLI FIMF_ECOLI]] Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Involved in the integration of FimH in the fimbriae.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The complex between the bacterial type 1 pilus subunit FimG and the peptide corresponding to the N-terminal extension (termed donor strand, Ds) of the partner subunit FimF (DsF) shows the strongest reported noncovalent molecular interaction, with a dissociation constant (KD ) of 1.5x10-20 m. However, the complex only exhibits a slow association rate of 330 m-1 s-1 that limits technical applications, such as its use in affinity purification. Herein, a structure-based approach was used to design pairs of FimGt (a FimG variant lacking its own N-terminal extension) and DsF variants with enhanced electrostatic surface complementarity. Association of the best mutant FimGt/DsF pairs was accelerated by more than two orders of magnitude, while the dissociation rates and 3D structures of the improved complexes remained essentially unperturbed. A KD value of 8.8x10-22 m was obtained for the best mutant complex, which is the lowest value reported to date for a protein/ligand complex.

-	~~Authors: Giese~~, C., Eras, J., Kern, A., Scharer~~, M.A.~~, Capitani, G., Glockshuber, R.	+	Accelerating the Association of the Most Stable Protein-Ligand Complex by more than Two Orders of Magnitude.,Giese C, Eras J, Kern A, Scharer MA, Capitani G, Glockshuber R Angew Chem Int Ed Engl. 2016 Jun 28. doi: 10.1002/anie.201603652. PMID:27351462<ref>PMID:27351462</ref>

-	~~Description: Crystal structure~~ of the E. ~~coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N~~-~~terminal FimG residues 1~~-~~12 truncated) in complex with the donor strand peptide DsF_SRIRIRGYVR~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5iqn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Capitani, G]]
	[[Category: Eras, J]]		[[Category: Eras, J]]
-	[[Category: Scharer, M.A]]
-	[[Category: Kern, A]]
-	[[Category: Glockshuber, R]]
	[[Category: Giese, C]]		[[Category: Giese, C]]
-	[[Category: ~~Capitani~~, G]]	+	[[Category: Glockshuber, R]]
		+	[[Category: Kern, A]]
		+	[[Category: Scharer, M A]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Complex]]
		+	[[Category: Fimgt]]
		+	[[Category: Protein]]

OCA: Protected "5iqn" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 00:48:06 GMT

Protected "5iqn" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:48, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5iqn is ON HOLD until Paper Publication Authors: Giese, C., Eras, J., Kern, A., Scharer, M.A., Capitani, G., Glockshuber, R. Description: Crystal s...

OCA — Wed, 11 May 2016 00:48:05 GMT

New page: '''Unreleased structure''' The entry 5iqn is ON HOLD until Paper Publication Authors: Giese, C., Eras, J., Kern, A., Scharer, M.A., Capitani, G., Glockshuber, R. Description: Crystal s...

New page

'''Unreleased structure'''

The entry 5iqn is ON HOLD until Paper Publication

Authors: Giese, C., Eras, J., Kern, A., Scharer, M.A., Capitani, G., Glockshuber, R.

Description: Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_SRIRIRGYVR
[[Category: Unreleased Structures]]
[[Category: Eras, J]]
[[Category: Scharer, M.A]]
[[Category: Kern, A]]
[[Category: Glockshuber, R]]
[[Category: Giese, C]]
[[Category: Capitani, G]]