5iwq - Revision history

OCA at 10:35, 6 September 2023

OCA — Wed, 06 Sep 2023 10:35:31 GMT

←Older revision		Revision as of 10:35, 6 September 2023
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	<StructureSection load='5iwq' size='340' side='right'caption='[[5iwq]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5iwq' size='340' side='right'caption='[[5iwq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5iwq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Corgl Corgl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5IWQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5iwq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum_ATCC_13032 Corynebacterium glutamicum ATCC 13032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IWQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~aspB, cg0294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=196627 CORGL])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iwq OCA], [https://pdbe.org/5iwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iwq RCSB], [https://www.ebi.ac.uk/pdbsum/5iwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iwq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5iwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iwq OCA], [~~http~~://pdbe.org/5iwq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5iwq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5iwq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5iwq ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ASPAT_CORGL ASPAT_CORGL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspartate transaminase]]~~	+	[[Category: Corynebacterium glutamicum ATCC 13032]]
-	~~[[Category: Corgl~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kim~~, K J~~]]	+	[[Category: Kim KJ]]
-	[[Category: Son~~, H F]]~~	+	[[Category: Son HF]]
-	~~[[Category: Aminotransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:20, 10 June 2020

OCA — Wed, 10 Jun 2020 07:20:26 GMT

←Older revision		Revision as of 07:20, 10 June 2020
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	==Crystal structure of aspartate aminotransferase (AspAT) from Corynebacterium glutamicum ATCC 13032==		==Crystal structure of aspartate aminotransferase (AspAT) from Corynebacterium glutamicum ATCC 13032==
-	<StructureSection load='5iwq' size='340' side='right' caption='[[5iwq]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5iwq' size='340' side='right'caption='[[5iwq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5iwq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IWQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5iwq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corgl Corgl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IWQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aspB, cg0294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196627 CORGL])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5iwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iwq OCA], [http://pdbe.org/5iwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iwq RCSB], [http://www.ebi.ac.uk/pdbsum/5iwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iwq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5iwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iwq OCA], [http://pdbe.org/5iwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iwq RCSB], [http://www.ebi.ac.uk/pdbsum/5iwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iwq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5iwq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5iwq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aspartate aminotransferase 3D structures\|Aspartate aminotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
		+	[[Category: Corgl]]
		+	[[Category: Large Structures]]
	[[Category: Kim, K J]]		[[Category: Kim, K J]]
	[[Category: Son, H F]]		[[Category: Son, H F]]
	[[Category: Aminotransferase]]		[[Category: Aminotransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 15:09, 26 July 2016

OCA — Tue, 26 Jul 2016 15:09:54 GMT

←Older revision		Revision as of 15:09, 26 July 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5iwq is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of aspartate aminotransferase (AspAT) from Corynebacterium glutamicum ATCC 13032==
		+	<StructureSection load='5iwq' size='340' side='right' caption='[[5iwq]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5iwq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IWQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iwq OCA], [http://pdbe.org/5iwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iwq RCSB], [http://www.ebi.ac.uk/pdbsum/5iwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iwq ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aspartate aminotransferase from Corynebacterium glutamicum (CgAspAT) is a PLP-dependent enzyme that catalyzes the production of L-aspartate and alpha-ketoglutarate from L-glutamate and oxaloacetate in L-lysine biosynthesis. In order to understand the molecular mechanism of CgAspAT and compare it with those of other aspartate aminotransferases (AspATs) from the aminotransferase class I, we determined the crystal structure of CgAspAT. CgAspAT functions as a dimer, and the CgAspAT monomer consists of two domains, the core domain and the auxiliary domain. The PLP cofactor is found to be bound to CgAspAT and stabilized through unique residues. In our current structure, a citrate molecule is bound at the active site of one molecule and mimics binding of the glutamate substrate. The residues involved in binding of the PLP cofactor and the glutamate substrate were confirmed by site-directed mutagenesis. Interestingly, compared with other AspATs from aminotransferase subgroup Ia and Ib, CgAspAT exhibited unique binding sites for both cofactor and substrate; moreover, it was found to have unusual structural features in the auxiliary domain. Based on these structural differences, we propose that CgAspAT does not belong to either subgroup Ia or Ib, and can be categorized into a subgroup Ic. The phylogenetic tree and RMSD analysis also indicates that CgAspAT is located in an independent AspAT subgroup.

-	~~Authors:~~ Son, H.-F.~~, Kim, K~~.-J.	+	Structural Insights into a Novel Class of Aspartate Aminotransferase from Corynebacterium glutamicum.,Son HF, Kim KJ PLoS One. 2016 Jun 29;11(6):e0158402. doi: 10.1371/journal.pone.0158402., eCollection 2016. PMID:27355211<ref>PMID:27355211</ref>

-	~~Description: Crystal structure~~ of ~~aspartate aminotransferase (AspAT) from Corynebacterium glutamicum ATCC 13032~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Kim, K.-J]]	+	<div class="pdbe-citations 5iwq" style="background-color:#fffaf0;"></div>
-	[[Category: Son, H.-F]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aspartate transaminase]]
		+	[[Category: Kim, K J]]
		+	[[Category: Son, H F]]
		+	[[Category: Aminotransferase]]
		+	[[Category: Transferase]]

OCA at 19:50, 23 June 2016

OCA — Thu, 23 Jun 2016 19:50:42 GMT

←Older revision		Revision as of 19:50, 23 June 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5iwq is ON HOLD	+	The entry 5iwq is ON HOLD until Paper Publication

	Authors: Son, H.-F., Kim, K.-J.		Authors: Son, H.-F., Kim, K.-J.

OCA at 12:55, 1 June 2016

OCA — Wed, 01 Jun 2016 12:55:12 GMT

←Older revision		Revision as of 12:55, 1 June 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5iwq is ON HOLD ~~until Paper Publication~~	+	The entry 5iwq is ON HOLD

	Authors: Son, H.-F., Kim, K.-J.		Authors: Son, H.-F., Kim, K.-J.

OCA: Protected "5iwq" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:05:22 GMT

Protected "5iwq" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:05, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5iwq is ON HOLD until Paper Publication Authors: Son, H.-F., Kim, K.-J. Description: Crystal structure of aspartate aminotransferase (AspAT) from C...

OCA — Wed, 11 May 2016 01:05:20 GMT

New page: '''Unreleased structure''' The entry 5iwq is ON HOLD until Paper Publication Authors: Son, H.-F., Kim, K.-J. Description: Crystal structure of aspartate aminotransferase (AspAT) from C...

New page

'''Unreleased structure'''

The entry 5iwq is ON HOLD until Paper Publication

Authors: Son, H.-F., Kim, K.-J.

Description: Crystal structure of aspartate aminotransferase (AspAT) from Corynebacterium glutamicum ATCC 13032
[[Category: Unreleased Structures]]
[[Category: Kim, K.-J]]
[[Category: Son, H.-F]]