5ixh - Revision history

OCA at 10:36, 6 September 2023

OCA — Wed, 06 Sep 2023 10:36:53 GMT

←Older revision		Revision as of 10:36, 6 September 2023
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	<StructureSection load='5ixh' size='340' side='right'caption='[[5ixh]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='5ixh' size='340' side='right'caption='[[5ixh]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ixh]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Burkholderia_cenocepacia_bc7~~ Burkholderia cenocepacia ~~bc7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5IXH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ixh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cenocepacia_BC7 Burkholderia cenocepacia BC7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IXH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OTP:(2E,6E,10E,14E,18E,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAENYL+TRIHYDROGEN+DIPHOSPHATE'>OTP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ixg\|5ixg]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OTP:(2E,6E,10E,14E,18E,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAENYL+TRIHYDROGEN+DIPHOSPHATE'>OTP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BURCENBC7_AP5370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=985077 Burkholderia cenocepacia BC7])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ixh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixh OCA], [https://pdbe.org/5ixh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ixh RCSB], [https://www.ebi.ac.uk/pdbsum/5ixh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixh ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ixh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixh OCA], [~~http~~://pdbe.org/5ixh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ixh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ixh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixh ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Burkholderia cenocepacia ~~bc7~~]]	+	[[Category: Burkholderia cenocepacia BC7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Loutet~~, S A~~]]	+	[[Category: Loutet SA]]
-	[[Category: Murphy~~, M E.P]]~~	+	[[Category: Murphy MEP]]
-	~~[[Category: Beta barrel lipocalin]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 07:21, 10 June 2020

OCA — Wed, 10 Jun 2020 07:21:08 GMT

←Older revision		Revision as of 07:21, 10 June 2020
Line 1:		Line 1:

	==Crystal Structure of Burkholderia cenocepacia BcnA==		==Crystal Structure of Burkholderia cenocepacia BcnA==
-	<StructureSection load='5ixh' size='340' side='right' caption='[[5ixh]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='5ixh' size='340' side='right'caption='[[5ixh]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ixh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXH OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IXH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ixh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_cenocepacia_bc7 Burkholderia cenocepacia bc7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IXH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OTP:(2E,6E,10E,14E,18E,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAENYL+TRIHYDROGEN+DIPHOSPHATE'>OTP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OTP:(2E,6E,10E,14E,18E,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAENYL+TRIHYDROGEN+DIPHOSPHATE'>OTP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ixg\|5ixg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ixg\|5ixg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ixh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixh OCA], [http://pdbe.org/5ixh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixh RCSB], [http://www.ebi.ac.uk/pdbsum/5ixh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixh ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BURCENBC7_AP5370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=985077 Burkholderia cenocepacia BC7])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ixh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixh OCA], [http://pdbe.org/5ixh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixh RCSB], [http://www.ebi.ac.uk/pdbsum/5ixh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixh ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Burkholderia cenocepacia bc7]]
		+	[[Category: Large Structures]]
	[[Category: Loutet, S A]]		[[Category: Loutet, S A]]
	[[Category: Murphy, M E.P]]		[[Category: Murphy, M E.P]]
	[[Category: Beta barrel lipocalin]]		[[Category: Beta barrel lipocalin]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 06:49, 29 March 2017

OCA — Wed, 29 Mar 2017 06:49:23 GMT

←Older revision		Revision as of 06:49, 29 March 2017
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ixh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixh OCA], [http://pdbe.org/5ixh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixh RCSB], [http://www.ebi.ac.uk/pdbsum/5ixh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ixh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixh OCA], [http://pdbe.org/5ixh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixh RCSB], [http://www.ebi.ac.uk/pdbsum/5ixh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixh ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The potential for microbes to overcome antibiotics of different classes before they reach bacterial cells is largely unexplored. Here we show that a soluble bacterial lipocalin produced by Burkholderia cenocepacia upon exposure to sublethal antibiotic concentrations increases resistance to diverse antibiotics in vitro and in vivo These phenotypes were recapitulated by heterologous expression in B. cenocepacia of lipocalin genes from Pseudomonas aeruginosa, Mycobacterium tuberculosis, and methicillin-resistant Staphylococcus aureus Purified lipocalin bound different classes of bactericidal antibiotics and contributed to bacterial survival in vivo Experimental and X-ray crystal structure-guided computational studies revealed that lipocalins counteract antibiotic action by capturing antibiotics in the extracellular space. We also demonstrated that fat-soluble vitamins prevent antibiotic capture by binding bacterial lipocalin with higher affinity than antibiotics. Therefore, bacterial lipocalins contribute to antimicrobial resistance by capturing diverse antibiotics in the extracellular space at the site of infection, which can be counteracted by known vitamins.IMPORTANCE Current research on antibiotic action and resistance focuses on targeting essential functions within bacterial cells. We discovered a previously unrecognized mode of general bacterial antibiotic resistance operating in the extracellular space, which depends on bacterial protein molecules called lipocalins. These molecules are highly conserved in most bacteria and have the ability to capture different classes of antibiotics outside bacterial cells. We also discovered that liposoluble vitamins, such as vitamin E, overcome in vitro and in vivo antibiotic resistance mediated by bacterial lipocalins, providing an unexpected new alternative to combat resistance by using this vitamin or its derivatives as antibiotic adjuvants.
		+
		+	Antibiotic Capture by Bacterial Lipocalins Uncovers an Extracellular Mechanism of Intrinsic Antibiotic Resistance.,El-Halfawy OM, Klett J, Ingram RJ, Loutet SA, Murphy ME, Martin-Santamaria S, Valvano MA MBio. 2017 Mar 14;8(2). pii: e00225-17. doi: 10.1128/mBio.00225-17. PMID:28292982<ref>PMID:28292982</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ixh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:21, 22 March 2017

OCA — Wed, 22 Mar 2017 16:21:28 GMT

←Older revision		Revision as of 16:21, 22 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ixh is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of Burkholderia cenocepacia BcnA==
-		+	<StructureSection load='5ixh' size='340' side='right' caption='[[5ixh]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors~~: ~~Loutet~~, S.A., ~~Murphy~~, M.E.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ixh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IXH FirstGlance]. <br>
-	~~Description: Crystal Structure of Burkholderia cenocepacia BcnA~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OTP:(2E,6E,10E,14E,18E,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAENYL+TRIHYDROGEN+DIPHOSPHATE'>OTP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ixg\|5ixg]]</td></tr>
-	[[Category: Murphy, M.E.P]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ixh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixh OCA], [http://pdbe.org/5ixh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixh RCSB], [http://www.ebi.ac.uk/pdbsum/5ixh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixh ProSAT]</span></td></tr>
-	[[Category: ~~Loutet, S.A~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Loutet, S A]]
		+	[[Category: Murphy, M E.P]]
		+	[[Category: Beta barrel lipocalin]]
		+	[[Category: Unknown function]]

OCA: Protected "5ixh" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:07:50 GMT

Protected "5ixh" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:07, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5ixh is ON HOLD until Paper Publication Authors: Loutet, S.A., Murphy, M.E.P. Description: Crystal Structure of Burkholderia cenocepacia BcnA [[Cat...

OCA — Wed, 11 May 2016 01:07:48 GMT

New page: '''Unreleased structure''' The entry 5ixh is ON HOLD until Paper Publication Authors: Loutet, S.A., Murphy, M.E.P. Description: Crystal Structure of Burkholderia cenocepacia BcnA [[Cat...

New page

'''Unreleased structure'''

The entry 5ixh is ON HOLD until Paper Publication

Authors: Loutet, S.A., Murphy, M.E.P.

Description: Crystal Structure of Burkholderia cenocepacia BcnA
[[Category: Unreleased Structures]]
[[Category: Murphy, M.E.P]]
[[Category: Loutet, S.A]]