5iz2 - Revision history

OCA at 10:39, 6 September 2023

OCA — Wed, 06 Sep 2023 10:39:32 GMT

←Older revision		Revision as of 10:39, 6 September 2023
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	<StructureSection load='5iz2' size='340' side='right'caption='[[5iz2]], [[Resolution\|resolution]] 2.02Å' scene=''>		<StructureSection load='5iz2' size='340' side='right'caption='[[5iz2]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5iz2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IZ2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5IZ2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5iz2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichonephila_clavipes Trichonephila clavipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IZ2 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5iz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iz2 OCA], [~~http~~://pdbe.org/5iz2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5iz2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5iz2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5iz2 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.02Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iz2 OCA], [https://pdbe.org/5iz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iz2 RCSB], [https://www.ebi.ac.uk/pdbsum/5iz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iz2 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B5SYS5_TRICX B5SYS5_TRICX]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Atkison, J H~~]]	+	[[Category: Trichonephila clavipes]]
-	[[Category: ~~Olsen, S K~~]]	+	[[Category: Atkison JH]]
-	[[Category: ~~Homodimer]]~~	+	[[Category: Olsen SK]]
-	~~[[Category: Major ampullate]]~~	+
-	~~[[Category: Spidroin]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 07:22, 10 June 2020

OCA — Wed, 10 Jun 2020 07:22:30 GMT

←Older revision		Revision as of 07:22, 10 June 2020
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	==Crystal structure of the N. clavipes spidroin NTD at pH 6.5==		==Crystal structure of the N. clavipes spidroin NTD at pH 6.5==
-	<StructureSection load='5iz2' size='340' side='right' caption='[[5iz2]], [[Resolution\|resolution]] 2.02Å' scene=''>	+	<StructureSection load='5iz2' size='340' side='right'caption='[[5iz2]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5iz2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IZ2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IZ2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5iz2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IZ2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IZ2 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5iz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iz2 OCA], [http://pdbe.org/5iz2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iz2 RCSB], [http://www.ebi.ac.uk/pdbsum/5iz2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iz2 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5iz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iz2 OCA], [http://pdbe.org/5iz2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iz2 RCSB], [http://www.ebi.ac.uk/pdbsum/5iz2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iz2 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Atkison, J H]]		[[Category: Atkison, J H]]
	[[Category: Olsen, S K]]		[[Category: Olsen, S K]]

OCA at 07:53, 10 August 2016

OCA — Wed, 10 Aug 2016 07:53:36 GMT

←Older revision		Revision as of 07:53, 10 August 2016
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iz2 OCA], [http://pdbe.org/5iz2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iz2 RCSB], [http://www.ebi.ac.uk/pdbsum/5iz2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iz2 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iz2 OCA], [http://pdbe.org/5iz2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iz2 RCSB], [http://www.ebi.ac.uk/pdbsum/5iz2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iz2 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Spider dragline silk is a natural polymer harboring unique physical and biochemical properties that make it an ideal biomaterial. Artificial silk production requires an understanding of the in vivo mechanisms spiders use to convert soluble proteins, called spidroins, into insoluble fibers. Controlled dimerization of the spidroin N-terminal domain (NTD) is crucial to this process. Here, we report the crystal structure of the Nephila clavipes major ampullate spidroin NTD dimer. Comparison of our structure to previously determined Euprosthenops australis structures reveals a subtle alteration in the structure of the NTD subunits that leads to differences in how the subunits are arranged at the dimer interface. We observe a subset of contacts that are specific to each ortholog, as well as a substantial increase in asymmetry in the interactions observed at the N. clavipes NTD dimer interface. These asymmetric interactions include novel intermolecular salt bridges that provide new insights into the mechanism of NTD dimerization. We also observe a unique intramolecular "handshake" interaction between two conserved acidic residues that our data suggest adds an additional layer of complexity to the pH-sensitive relay mechanism for NTD dimerization. The results of a panel of tryptophan fluorescence dimerization assays probing the importance of these interactions support our structural observations. Based on our findings, we propose that conformational selectivity and plasticity at the NTD dimer interface play a role in the pH-dependent transition of the NTD from monomer to stably associated dimer as the spidroin progresses through the silk extrusion duct.
		+
		+	Crystal Structure of the Nephila clavipes Major Ampullate Spidroin 1A N-terminal Domain Reveals Plasticity at the Dimer Interface.,Atkison JH, Parnham S, Marcotte WR Jr, Olsen SK J Biol Chem. 2016 Jul 21. pii: jbc.M116.736710. PMID:27445329<ref>PMID:27445329</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5iz2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:44, 27 July 2016

OCA — Wed, 27 Jul 2016 15:44:50 GMT

←Older revision		Revision as of 15:44, 27 July 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5iz2 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the N. clavipes spidroin NTD at pH 6.5==
-		+	<StructureSection load='5iz2' size='340' side='right' caption='[[5iz2]], [[Resolution\|resolution]] 2.02Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5iz2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IZ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IZ2 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iz2 OCA], [http://pdbe.org/5iz2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iz2 RCSB], [http://www.ebi.ac.uk/pdbsum/5iz2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iz2 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Atkison, J H]]
		+	[[Category: Olsen, S K]]
		+	[[Category: Homodimer]]
		+	[[Category: Major ampullate]]
		+	[[Category: Spidroin]]
		+	[[Category: Structural protein]]

OCA at 14:53, 15 May 2016

OCA — Sun, 15 May 2016 14:53:39 GMT

←Older revision		Revision as of 14:53, 15 May 2016
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	The entry 5iz2 is ON HOLD until Paper Publication		The entry 5iz2 is ON HOLD until Paper Publication

-	Authors: ~~Atkison, J.H., Olsen, S.K.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Olsen, S.K]]
-	[[Category: Atkison, J.H]]

OCA: Protected "5iz2" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:11:33 GMT

Protected "5iz2" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:11, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5iz2 is ON HOLD until Paper Publication Authors: Atkison, J.H., Olsen, S.K. Description: Category: Unreleased Structures [[Category: Olsen, S....

OCA — Wed, 11 May 2016 01:11:32 GMT

New page: '''Unreleased structure''' The entry 5iz2 is ON HOLD until Paper Publication Authors: Atkison, J.H., Olsen, S.K. Description: Category: Unreleased Structures [[Category: Olsen, S....

New page

'''Unreleased structure'''

The entry 5iz2 is ON HOLD until Paper Publication

Authors: Atkison, J.H., Olsen, S.K.

Description:
[[Category: Unreleased Structures]]
[[Category: Olsen, S.K]]
[[Category: Atkison, J.H]]