5j1j - Revision history

OCA at 09:17, 20 March 2024

OCA — Wed, 20 Mar 2024 09:17:20 GMT

←Older revision		Revision as of 09:17, 20 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/FLEN_PSEAE FLEN_PSEAE] ATPase that plays an important role in maintaining flagellar number in Pseudomonas aeruginosa (PubMed:10629180, PubMed:28065505). Exhibits anti-activator activity against FleQ, the global transcriptional regulator of flagellar genes (PubMed:22581773, PubMed:28065505).<ref>PMID:10629180</ref> <ref>PMID:22581773</ref> <ref>PMID:28065505</ref>		[https://www.uniprot.org/uniprot/FLEN_PSEAE FLEN_PSEAE] ATPase that plays an important role in maintaining flagellar number in Pseudomonas aeruginosa (PubMed:10629180, PubMed:28065505). Exhibits anti-activator activity against FleQ, the global transcriptional regulator of flagellar genes (PubMed:22581773, PubMed:28065505).<ref>PMID:10629180</ref> <ref>PMID:22581773</ref> <ref>PMID:28065505</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	FleN, a P loop ATPase is vital for maintaining a monotrichous phenotype in Pseudomonas aeruginosa. FleN exhibits antagonistic activity against FleQ, the master transcriptional regulator of flagellar genes. Crystal structures of FleN in the apo form (1.66 A) and in complex with beta,gamma-imidoadenosine 5'-triphosphate (1.55 A) reveal that it undergoes drastic conformational changes on ATP binding to attain a structure capable of dimerization. Mutations of the residues that stabilize the binding of ATP were defective in their ability to dimerize and do not inhibit ATP hydrolysis by FleQ. Conversely, the catalytic mutant of FleN, was an efficient inhibitor. These observations posit that the dimer is the functional form of FleN and it is nucleotide binding and not hydrolysis by FleN that is necessary to exert an antagonistic effect against FleQ. Our study shows that ATP-induced dimerization may be a strategy to achieve reversible inhibition of FleQ to fine-tune the function of this activator to an optimal level.
-
-	ATP-Induced Structural Remodeling in the Antiactivator FleN Enables Formation of the Functional Dimeric Form.,Chanchal, Banerjee P, Jain D Structure. 2017 Feb 7;25(2):243-252. doi: 10.1016/j.str.2016.11.022. Epub 2017, Jan 5. PMID:28065505<ref>PMID:28065505</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5j1j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:42, 6 September 2023

OCA — Wed, 06 Sep 2023 10:42:12 GMT

←Older revision		Revision as of 10:42, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='5j1j' size='340' side='right'caption='[[5j1j]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='5j1j' size='340' side='right'caption='[[5j1j]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j1j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5J1J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j1j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J1J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fleN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5j1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1j OCA], [~~http~~://pdbe.org/5j1j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5j1j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5j1j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1j OCA], [https://pdbe.org/5j1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j1j RCSB], [https://www.ebi.ac.uk/pdbsum/5j1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FLEN_PSEAE FLEN_PSEAE] ATPase that plays an important role in maintaining flagellar number in Pseudomonas aeruginosa (PubMed:10629180, PubMed:28065505). Exhibits anti-activator activity against FleQ, the global transcriptional regulator of flagellar genes (PubMed:22581773, PubMed:28065505).<ref>PMID:10629180</ref> <ref>PMID:22581773</ref> <ref>PMID:28065505</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Banerjee, P]]	+	[[Category: Banerjee P]]
	[[Category: Chanchal]]		[[Category: Chanchal]]
-	[[Category: Jain, D]]	+	[[Category: Jain D]]
-	~~[[Category: Amppnp]]~~	+
-	~~[[Category: Antiactivator]]~~	+
-	~~[[Category: Flen]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 07:24, 10 June 2020

OCA — Wed, 10 Jun 2020 07:24:56 GMT

←Older revision		Revision as of 07:24, 10 June 2020
Line 1:		Line 1:

	==Structure of FleN-AMPPNP complex==		==Structure of FleN-AMPPNP complex==
-	<StructureSection load='5j1j' size='340' side='right' caption='[[5j1j]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='5j1j' size='340' side='right'caption='[[5j1j]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j1j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1J OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5J1J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j1j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J1J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5j1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1j OCA], [http://pdbe.org/5j1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j1j RCSB], [http://www.ebi.ac.uk/pdbsum/5j1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1j ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fleN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1j OCA], [http://pdbe.org/5j1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j1j RCSB], [http://www.ebi.ac.uk/pdbsum/5j1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseae]]
	[[Category: Banerjee, P]]		[[Category: Banerjee, P]]
	[[Category: Chanchal]]		[[Category: Chanchal]]

OCA at 13:45, 29 March 2017

OCA — Wed, 29 Mar 2017 13:45:12 GMT

←Older revision		Revision as of 13:45, 29 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5j1j is ~~ON HOLD until Paper~~ Publication	+	==Structure of FleN-AMPPNP complex==
		+	<StructureSection load='5j1j' size='340' side='right' caption='[[5j1j]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5j1j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J1J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1j OCA], [http://pdbe.org/5j1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j1j RCSB], [http://www.ebi.ac.uk/pdbsum/5j1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1j ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	FleN, a P loop ATPase is vital for maintaining a monotrichous phenotype in Pseudomonas aeruginosa. FleN exhibits antagonistic activity against FleQ, the master transcriptional regulator of flagellar genes. Crystal structures of FleN in the apo form (1.66 A) and in complex with beta,gamma-imidoadenosine 5'-triphosphate (1.55 A) reveal that it undergoes drastic conformational changes on ATP binding to attain a structure capable of dimerization. Mutations of the residues that stabilize the binding of ATP were defective in their ability to dimerize and do not inhibit ATP hydrolysis by FleQ. Conversely, the catalytic mutant of FleN, was an efficient inhibitor. These observations posit that the dimer is the functional form of FleN and it is nucleotide binding and not hydrolysis by FleN that is necessary to exert an antagonistic effect against FleQ. Our study shows that ATP-induced dimerization may be a strategy to achieve reversible inhibition of FleQ to fine-tune the function of this activator to an optimal level.

-	~~Authors~~:	+	ATP-Induced Structural Remodeling in the Antiactivator FleN Enables Formation of the Functional Dimeric Form.,Chanchal, Banerjee P, Jain D Structure. 2017 Feb 7;25(2):243-252. doi: 10.1016/j.str.2016.11.022. Epub 2017, Jan 5. PMID:28065505<ref>PMID:28065505</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5j1j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Banerjee, P]]
		+	[[Category: Chanchal]]
		+	[[Category: Jain, D]]
		+	[[Category: Amppnp]]
		+	[[Category: Antiactivator]]
		+	[[Category: Flen]]
		+	[[Category: Transcription]]

OCA at 14:58, 15 May 2016

OCA — Sun, 15 May 2016 14:58:04 GMT

←Older revision		Revision as of 14:58, 15 May 2016
Line 3:		Line 3:
	The entry 5j1j is ON HOLD until Paper Publication		The entry 5j1j is ON HOLD until Paper Publication

-	Authors: ~~Jain, D., Chanchal, Banerjee, P.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Chanchal, Banerjee, P]]
-	[[Category: Jain, D]]

OCA: Protected "5j1j" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:19:07 GMT

Protected "5j1j" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:19, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5j1j is ON HOLD until Paper Publication Authors: Jain, D., Chanchal, Banerjee, P. Description: Category: Unreleased Structures [[Category: Cha...

OCA — Wed, 11 May 2016 01:19:06 GMT

New page: '''Unreleased structure''' The entry 5j1j is ON HOLD until Paper Publication Authors: Jain, D., Chanchal, Banerjee, P. Description: Category: Unreleased Structures [[Category: Cha...

New page

'''Unreleased structure'''

The entry 5j1j is ON HOLD until Paper Publication

Authors: Jain, D., Chanchal, Banerjee, P.

Description:
[[Category: Unreleased Structures]]
[[Category: Chanchal, Banerjee, P]]
[[Category: Jain, D]]