5j1p - Revision history

OCA at 10:42, 6 September 2023

OCA — Wed, 06 Sep 2023 10:42:20 GMT

←Older revision		Revision as of 10:42, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='5j1p' size='340' side='right'caption='[[5j1p]], [[Resolution\|resolution]] 2.36Å' scene=''>		<StructureSection load='5j1p' size='340' side='right'caption='[[5j1p]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j1p]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lassa_mammarenavirus Lassa mammarenavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5J1P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lassa_mammarenavirus Lassa mammarenavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J1P FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.36Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5izh\|5izh]], [[5ize\|5ize]], [[5j1n\|5j1n]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1p OCA], [https://pdbe.org/5j1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j1p RCSB], [https://www.ebi.ac.uk/pdbsum/5j1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1p ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5j1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1p OCA], [~~http~~://pdbe.org/5j1p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5j1p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5j1p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1p ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/Q6GWS6_9VIRU Q6GWS6_9VIRU]] RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.[PIRNR:PIRNR000836]	+	[https://www.uniprot.org/uniprot/L_LASV L_LASV]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 29:		Line 28:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lassa mammarenavirus]]		[[Category: Lassa mammarenavirus]]
-	~~[[Category: RNA-directed RNA polymerase]]~~	+	[[Category: Cusack S]]
-	[[Category: Cusack, S]]	+	[[Category: Reguera J]]
-	[[Category: Reguera, J]]	+
-	~~[[Category: Cap-snatching nuclease lassa transcription]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:25, 10 June 2020

OCA — Wed, 10 Jun 2020 07:25:10 GMT

←Older revision		Revision as of 07:25, 10 June 2020
Line 1:		Line 1:

	==Lassa virus L protein cap-snatching endonuclease. Bound to two manganese ions==		==Lassa virus L protein cap-snatching endonuclease. Bound to two manganese ions==
-	<StructureSection load='5j1p' size='340' side='right' caption='[[5j1p]], [[Resolution\|resolution]] 2.36Å' scene=''>	+	<StructureSection load='5j1p' size='340' side='right'caption='[[5j1p]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j1p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1P OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5J1P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j1p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lassa_mammarenavirus Lassa mammarenavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J1P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5izh\|5izh]], [[5ize\|5ize]], [[5j1n\|5j1n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5izh\|5izh]], [[5ize\|5ize]], [[5j1n\|5j1n]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5j1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1p OCA], [http://pdbe.org/5j1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j1p RCSB], [http://www.ebi.ac.uk/pdbsum/5j1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1p OCA], [http://pdbe.org/5j1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j1p RCSB], [http://www.ebi.ac.uk/pdbsum/5j1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5j1p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5j1p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lassa mammarenavirus]]
	[[Category: RNA-directed RNA polymerase]]		[[Category: RNA-directed RNA polymerase]]
	[[Category: Cusack, S]]		[[Category: Cusack, S]]

OCA at 10:26, 13 July 2016

OCA — Wed, 13 Jul 2016 10:26:36 GMT

←Older revision		Revision as of 10:26, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5j1p is ~~ON HOLD until Paper~~ Publication	+	==Lassa virus L protein cap-snatching endonuclease. Bound to two manganese ions==
		+	<StructureSection load='5j1p' size='340' side='right' caption='[[5j1p]], [[Resolution\|resolution]] 2.36Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5j1p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J1P FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5izh\|5izh]], [[5ize\|5ize]], [[5j1n\|5j1n]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j1p OCA], [http://pdbe.org/5j1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j1p RCSB], [http://www.ebi.ac.uk/pdbsum/5j1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j1p ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q6GWS6_9VIRU Q6GWS6_9VIRU]] RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.[PIRNR:PIRNR000836]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Segmented negative strand RNA viruses of the arena-, bunya- and orthomyxovirus families uniquely carry out viral mRNA transcription by the cap-snatching mechanism. This involves cleavage of host mRNAs close to their capped 5' end by an endonuclease (EN) domain located in the N-terminal region of the viral polymerase. We present the structure of the cap-snatching EN of Hantaan virus, a bunyavirus belonging to hantavirus genus. Hantaan EN has an active site configuration, including a metal co-ordinating histidine, and nuclease activity similar to the previously reported La Crosse virus and Influenza virus ENs (orthobunyavirus and orthomyxovirus respectively), but is more active in cleaving a double stranded RNA substrate. In contrast, Lassa arenavirus EN has only acidic metal co-ordinating residues. We present three high resolution structures of Lassa virus EN with different bound ion configurations and show in comparative biophysical and biochemical experiments with Hantaan, La Crosse and influenza ENs that the isolated Lassa EN is essentially inactive. The results are discussed in the light of EN activation mechanisms revealed by recent structures of full-length influenza virus polymerase.

-	~~Authors: Reguera~~, J., ~~Cusack~~, S.	+	Comparative Structural and Functional Analysis of Bunyavirus and Arenavirus Cap-Snatching Endonucleases.,Reguera J, Gerlach P, Rosenthal M, Gaudon S, Coscia F, Gunther S, Cusack S PLoS Pathog. 2016 Jun 15;12(6):e1005636. doi: 10.1371/journal.ppat.1005636., eCollection 2016 Jun. PMID:27304209<ref>PMID:27304209</ref>

-	~~Description: Lassa virus L protein cap-snatching endonuclease~~. ~~Bound to two manganese ions~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Reguera, J~~]]	+	<div class="pdbe-citations 5j1p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: RNA-directed RNA polymerase]]
	[[Category: Cusack, S]]		[[Category: Cusack, S]]
		+	[[Category: Reguera, J]]
		+	[[Category: Cap-snatching nuclease lassa transcription]]
		+	[[Category: Transferase]]

OCA: Protected "5j1p" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:19:35 GMT

Protected "5j1p" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:19, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5j1p is ON HOLD until Paper Publication Authors: Reguera, J., Cusack, S. Description: Lassa virus L protein cap-snatching endonuclease. Bound to tw...

OCA — Wed, 11 May 2016 01:19:34 GMT

New page: '''Unreleased structure''' The entry 5j1p is ON HOLD until Paper Publication Authors: Reguera, J., Cusack, S. Description: Lassa virus L protein cap-snatching endonuclease. Bound to tw...

New page

'''Unreleased structure'''

The entry 5j1p is ON HOLD until Paper Publication

Authors: Reguera, J., Cusack, S.

Description: Lassa virus L protein cap-snatching endonuclease. Bound to two manganese ions
[[Category: Unreleased Structures]]
[[Category: Reguera, J]]
[[Category: Cusack, S]]