5j8q - Revision history

OCA at 10:54, 6 September 2023

OCA — Wed, 06 Sep 2023 10:54:32 GMT

←Older revision		Revision as of 10:54, 6 September 2023
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	<StructureSection load='5j8q' size='340' side='right'caption='[[5j8q]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5j8q' size='340' side='right'caption='[[5j8q]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j8q]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J8Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5J8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j8q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J8Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ALA:ALANINE'>ALA</scene>~~, ~~<scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.702Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~CSS~~:~~S-MERCAPTOCYSTEINE~~'>~~CSS~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">sufS~~, ~~csd, yurW, BSU32690 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j8q OCA], [https://pdbe.org/5j8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j8q RCSB], [https://www.ebi.ac.uk/pdbsum/5j8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j8q ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5j8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j8q OCA], [~~http~~://pdbe.org/5j8q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5j8q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5j8q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5j8q ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SUFS_BACSU SUFS_BACSU]] Enzyme able to deliver sulfur to partners involved in Fe-S cluster assembly. Catalyzes the removal of elemental sulfur atoms from L-cysteine to produce L-alanine. Activity is stimulated 40-to 100-fold by SufU, which acts as a second substrate for this enzyme following release of Ala, and generating SufU.S. A mixture of SufS, SufU, Fra and L-cysteine is able to reconstitute Fe-S clusters on apo-aconitase (citB), reconstituting aconitase activity.<ref>PMID:20097860</ref> <ref>PMID:20822158</ref> <ref>PMID:21744456</ref>	+	[https://www.uniprot.org/uniprot/SUFS_BACSU SUFS_BACSU] Enzyme able to deliver sulfur to partners involved in Fe-S cluster assembly. Catalyzes the removal of elemental sulfur atoms from L-cysteine to produce L-alanine. Activity is stimulated 40-to 100-fold by SufU, which acts as a second substrate for this enzyme following release of Ala, and generating SufU.S. A mixture of SufS, SufU, Fra and L-cysteine is able to reconstitute Fe-S clusters on apo-aconitase (citB), reconstituting aconitase activity.<ref>PMID:20097860</ref> <ref>PMID:20822158</ref> <ref>PMID:21744456</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu]]~~	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	~~[[Category: Cysteine desulfurase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Altegoer, F]]	+	[[Category: Altegoer F]]
-	[[Category: Bange, G]]	+	[[Category: Bange G]]
-	~~[[Category: Iron sulfur cluster]]~~	+
-	~~[[Category: Suf-system]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:33, 10 June 2020

OCA — Wed, 10 Jun 2020 07:33:35 GMT

←Older revision		Revision as of 07:33, 10 June 2020
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	==Crystal Structure of the Cysteine Desulfurase SufS of Bacillus subtilis==		==Crystal Structure of the Cysteine Desulfurase SufS of Bacillus subtilis==
-	<StructureSection load='5j8q' size='340' side='right' caption='[[5j8q]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5j8q' size='340' side='right'caption='[[5j8q]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j8q]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J8Q OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5J8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j8q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J8Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J8Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sufS, csd, yurW, BSU32690 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5j8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j8q OCA], [http://pdbe.org/5j8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j8q RCSB], [http://www.ebi.ac.uk/pdbsum/5j8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j8q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j8q OCA], [http://pdbe.org/5j8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j8q RCSB], [http://www.ebi.ac.uk/pdbsum/5j8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j8q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5j8q" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5j8q" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cysteine desulfurase\|Cysteine desulfurase]]
		+	*[[Cysteine desulfurase 3D structures\|Cysteine desulfurase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacsu]]
	[[Category: Cysteine desulfurase]]		[[Category: Cysteine desulfurase]]
		+	[[Category: Large Structures]]
	[[Category: Altegoer, F]]		[[Category: Altegoer, F]]
	[[Category: Bange, G]]		[[Category: Bange, G]]

OCA at 15:45, 27 July 2016

OCA — Wed, 27 Jul 2016 15:45:17 GMT

←Older revision		Revision as of 15:45, 27 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5j8q is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal Structure of the Cysteine Desulfurase SufS of Bacillus subtilis==
		+	<StructureSection load='5j8q' size='340' side='right' caption='[[5j8q]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5j8q]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J8Q FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j8q OCA], [http://pdbe.org/5j8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j8q RCSB], [http://www.ebi.ac.uk/pdbsum/5j8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j8q ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SUFS_BACSU SUFS_BACSU]] Enzyme able to deliver sulfur to partners involved in Fe-S cluster assembly. Catalyzes the removal of elemental sulfur atoms from L-cysteine to produce L-alanine. Activity is stimulated 40-to 100-fold by SufU, which acts as a second substrate for this enzyme following release of Ala, and generating SufU.S. A mixture of SufS, SufU, Fra and L-cysteine is able to reconstitute Fe-S clusters on apo-aconitase (citB), reconstituting aconitase activity.<ref>PMID:20097860</ref> <ref>PMID:20822158</ref> <ref>PMID:21744456</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The biosynthesis of iron sulfur (Fe-S) clusters in Bacillus subtilis is mediated by a SUF-type gene cluster, consisting of the cysteine desulfurase SufS, the scaffold protein SufU, and the putative chaperone complex SufB/SufC/SufD. Here, we present the high-resolution crystal structure of the SufS homodimer in its product-bound state (i.e., in complex with pyrodoxal-5'-phosphate, alanine, Cys361-persulfide). By performing hydrogen/deuterium exchange (H/DX) experiments, we characterized the interaction of SufS with SufU and demonstrate that SufU induces an opening of the active site pocket of SufS. Recent data indicate that frataxin could be involved in Fe-S cluster biosynthesis by facilitating iron incorporation. H/DX experiments show that frataxin indeed interacts with the SufS/SufU complex at the active site. Our findings deepen the current understanding of Fe-S cluster biosynthesis, a complex yet essential process, in the model organism B. subtilis.

-	~~Authors: Altegoer~~, F., ~~Bange~~, G.	+	Crystal Structure of Bacillus subtilis Cysteine Desulfurase SufS and Its Dynamic Interaction with Frataxin and Scaffold Protein SufU.,Blauenburg B, Mielcarek A, Altegoer F, Fage CD, Linne U, Bange G, Marahiel MA PLoS One. 2016 Jul 6;11(7):e0158749. doi: 10.1371/journal.pone.0158749., eCollection 2016. PMID:27382962<ref>PMID:27382962</ref>

-	~~Description: Crystal Structure~~ of the ~~Cysteine Desulfurase SufS~~ of ~~Bacillus subtilis~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Bange, G~~]]	+	<div class="pdbe-citations 5j8q" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cysteine desulfurase]]
	[[Category: Altegoer, F]]		[[Category: Altegoer, F]]
		+	[[Category: Bange, G]]
		+	[[Category: Iron sulfur cluster]]
		+	[[Category: Suf-system]]
		+	[[Category: Transferase]]

OCA: Protected "5j8q" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:41:09 GMT

Protected "5j8q" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:41, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5j8q is ON HOLD until Paper Publication Authors: Altegoer, F., Bange, G. Description: Crystal Structure of the Cysteine Desulfurase SufS of Bacillu...

OCA — Wed, 11 May 2016 01:41:08 GMT

New page: '''Unreleased structure''' The entry 5j8q is ON HOLD until Paper Publication Authors: Altegoer, F., Bange, G. Description: Crystal Structure of the Cysteine Desulfurase SufS of Bacillu...

New page

'''Unreleased structure'''

The entry 5j8q is ON HOLD until Paper Publication

Authors: Altegoer, F., Bange, G.

Description: Crystal Structure of the Cysteine Desulfurase SufS of Bacillus subtilis
[[Category: Unreleased Structures]]
[[Category: Bange, G]]
[[Category: Altegoer, F]]