5j9s - Revision history

OCA at 10:56, 6 September 2023

OCA — Wed, 06 Sep 2023 10:56:09 GMT

←Older revision		Revision as of 10:56, 6 September 2023
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	<StructureSection load='5j9s' size='340' side='right'caption='[[5j9s]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5j9s' size='340' side='right'caption='[[5j9s]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j9s]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J9S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5J9S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j9s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J9S FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.702Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">MLLT1, ENL, LTG19, YEATS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j9s OCA], [https://pdbe.org/5j9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j9s RCSB], [https://www.ebi.ac.uk/pdbsum/5j9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j9s ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5j9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j9s OCA], [~~http~~://pdbe.org/5j9s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5j9s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5j9s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5j9s ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/ENL_HUMAN ENL_HUMAN]] A chromosomal aberration involving MLLT1 is associated with acute leukemias. Translocation t(11;19)(q23;p13.3) with KMT2A/MLL1. The result is a rogue activator protein.	+	[https://www.uniprot.org/uniprot/ENL_HUMAN ENL_HUMAN] A chromosomal aberration involving MLLT1 is associated with acute leukemias. Translocation t(11;19)(q23;p13.3) with KMT2A/MLL1. The result is a rogue activator protein.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ENL_HUMAN ENL_HUMAN]] Component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.<ref>PMID:20159561</ref> <ref>PMID:20471948</ref>	+	[https://www.uniprot.org/uniprot/ENL_HUMAN ENL_HUMAN] Component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.<ref>PMID:20159561</ref> <ref>PMID:20471948</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Li, H]]	+	[[Category: Li H]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Enl yeat]]~~	+
-	~~[[Category: H3k27]]~~	+
-	~~[[Category: Histone acetyllysine]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 07:38, 10 June 2020

OCA — Wed, 10 Jun 2020 07:38:11 GMT

←Older revision		Revision as of 07:38, 10 June 2020
Line 1:		Line 1:

	==ENL YEATS in complex with histone H3 acetylation at K27==		==ENL YEATS in complex with histone H3 acetylation at K27==
-	<StructureSection load='5j9s' size='340' side='right' caption='[[5j9s]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5j9s' size='340' side='right'caption='[[5j9s]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j9s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J9S OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5J9S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j9s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J9S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J9S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5j9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j9s OCA], [http://pdbe.org/5j9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j9s RCSB], [http://www.ebi.ac.uk/pdbsum/5j9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j9s ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MLLT1, ENL, LTG19, YEATS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j9s OCA], [http://pdbe.org/5j9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j9s RCSB], [http://www.ebi.ac.uk/pdbsum/5j9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j9s ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Li, H]]		[[Category: Li, H]]
	[[Category: Li, Y]]		[[Category: Li, Y]]

OCA at 16:42, 9 March 2017

OCA — Thu, 09 Mar 2017 16:42:12 GMT

←Older revision		Revision as of 16:42, 9 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5j9s is ~~ON HOLD~~	+	==ENL YEATS in complex with histone H3 acetylation at K27==
		+	<StructureSection load='5j9s' size='340' side='right' caption='[[5j9s]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5j9s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J9S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j9s OCA], [http://pdbe.org/5j9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j9s RCSB], [http://www.ebi.ac.uk/pdbsum/5j9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j9s ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/ENL_HUMAN ENL_HUMAN]] A chromosomal aberration involving MLLT1 is associated with acute leukemias. Translocation t(11;19)(q23;p13.3) with KMT2A/MLL1. The result is a rogue activator protein.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENL_HUMAN ENL_HUMAN]] Component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.<ref>PMID:20159561</ref> <ref>PMID:20471948</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cancer cells are characterized by aberrant epigenetic landscapes and often exploit chromatin machinery to activate oncogenic gene expression programs. Recognition of modified histones by 'reader' proteins constitutes a key mechanism underlying these processes; therefore, targeting such pathways holds clinical promise, as exemplified by the development of bromodomain and extra-terminal (BET) inhibitors. We recently identified the YEATS domain as an acetyl-lysine-binding module, but its functional importance in human cancer remains unknown. Here we show that the YEATS domain-containing protein ENL, but not its paralogue AF9, is required for disease maintenance in acute myeloid leukaemia. CRISPR-Cas9-mediated depletion of ENL led to anti-leukaemic effects, including increased terminal myeloid differentiation and suppression of leukaemia growth in vitro and in vivo. Biochemical and crystal structural studies and chromatin-immunoprecipitation followed by sequencing analyses revealed that ENL binds to acetylated histone H3, and co-localizes with H3K27ac and H3K9ac on the promoters of actively transcribed genes that are essential for leukaemia. Disrupting the interaction between the YEATS domain and histone acetylation via structure-based mutagenesis reduced the recruitment of RNA polymerase II to ENL-target genes, leading to the suppression of oncogenic gene expression programs. Notably, disrupting the functionality of ENL further sensitized leukaemia cells to BET inhibitors. Together, our data identify ENL as a histone acetylation reader that regulates oncogenic transcriptional programs in acute myeloid leukaemia, and suggest that displacement of ENL from chromatin may be a promising epigenetic therapy, alone or in combination with BET inhibitors, for aggressive leukaemia.

-	~~Authors~~:	+	ENL links histone acetylation to oncogenic gene expression in acute myeloid leukaemia.,Wan L, Wen H, Li Y, Lyu J, Xi Y, Hoshii T, Joseph JK, Wang X, Loh YE, Erb MA, Souza AL, Bradner JE, Shen L, Li W, Li H, Allis CD, Armstrong SA, Shi X Nature. 2017 Mar 1. doi: 10.1038/nature21687. PMID:28241141<ref>PMID:28241141</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5j9s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Li, H]]
		+	[[Category: Li, Y]]
		+	[[Category: Complex]]
		+	[[Category: Enl yeat]]
		+	[[Category: H3k27]]
		+	[[Category: Histone acetyllysine]]
		+	[[Category: Transcription]]

OCA at 15:12, 15 May 2016

OCA — Sun, 15 May 2016 15:12:21 GMT

←Older revision		Revision as of 15:12, 15 May 2016
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	The entry 5j9s is ON HOLD		The entry 5j9s is ON HOLD

-	Authors: ~~Li, Y., Li, H.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Li, Y]]
-	[[Category: Li, H]]

OCA: Protected "5j9s" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:44:05 GMT

Protected "5j9s" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:44, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5j9s is ON HOLD Authors: Li, Y., Li, H. Description: Category: Unreleased Structures Category: Li, Y Category: Li, H

OCA — Wed, 11 May 2016 01:44:04 GMT

New page: '''Unreleased structure''' The entry 5j9s is ON HOLD Authors: Li, Y., Li, H. Description: Category: Unreleased Structures Category: Li, Y Category: Li, H

New page

'''Unreleased structure'''

The entry 5j9s is ON HOLD

Authors: Li, Y., Li, H.

Description:
[[Category: Unreleased Structures]]
[[Category: Li, Y]]
[[Category: Li, H]]