5jbl - Revision history

OCA at 12:36, 6 March 2024

OCA — Wed, 06 Mar 2024 12:36:50 GMT

←Older revision		Revision as of 12:36, 6 March 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PCPP_BPT4 PCPP_BPT4] The pathway of bacteriophage T4 head assembly begins with the formation of a prohead bound to the bacterial cell membrane which is later converted to the mature, DNA-containing head. During maturation, all but one of the prohead proteins are proteolytically processed by a phage-coded protease which is formed by autocatalytic cleavage of the product of gene 21 (gp21). Protease gp21 has been tentatively located in the center of the prohead core.		[https://www.uniprot.org/uniprot/PCPP_BPT4 PCPP_BPT4] The pathway of bacteriophage T4 head assembly begins with the formation of a prohead bound to the bacterial cell membrane which is later converted to the mature, DNA-containing head. During maturation, all but one of the prohead proteins are proteolytically processed by a phage-coded protease which is formed by autocatalytic cleavage of the product of gene 21 (gp21). Protease gp21 has been tentatively located in the center of the prohead core.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Many large viruses, including tailed dsDNA bacteriophages and herpesviruses, assemble their capsids via formation of precursors, called procapsids or proheads. The prohead has an internal core, made of scaffolding proteins, and an outer shell, formed by the major capsid protein. The prohead usually contains a protease, which is activated during capsid maturation to destroy the inner core and liberate space for the genome. Here, we report a 2.0 A resolution structure of the pentameric procapsid protease of bacteriophage T4, gene product (gp)21. The structure corresponds to the enzyme's pre-active state in which its N-terminal region blocks the catalytic center, demonstrating that the activation mechanism involves self-cleavage of nine N-terminal residues. We describe similarities and differences between T4 gp21 and related herpesvirus proteases. We found that gp21 and the herpesvirus proteases have similarity with proteins forming the tubes of phage tails and bacterial type VI secretion systems, suggesting their common evolutionary origin.
-
-	Common Evolutionary Origin of Procapsid Proteases, Phage Tail Tubes, and Tubes of Bacterial Type VI Secretion Systems.,Fokine A, Rossmann MG Structure. 2016 Nov 1;24(11):1928-1935. doi: 10.1016/j.str.2016.08.013. Epub 2016, Sep 22. PMID:27667692<ref>PMID:27667692</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5jbl" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:58, 6 September 2023

OCA — Wed, 06 Sep 2023 10:58:17 GMT

←Older revision		Revision as of 10:58, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='5jbl' size='340' side='right'caption='[[5jbl]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='5jbl' size='340' side='right'caption='[[5jbl]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jbl]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bpt4 Bpt4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JBL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JBL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jbl]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JBL FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~21 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10665 BPT4])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.943Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jbl OCA], [~~http~~://pdbe.org/5jbl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jbl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jbl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jbl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jbl OCA], [https://pdbe.org/5jbl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jbl RCSB], [https://www.ebi.ac.uk/pdbsum/5jbl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jbl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PCPP_BPT4 PCPP_BPT4]] The pathway of bacteriophage T4 head assembly begins with the formation of a prohead bound to the bacterial cell membrane which is later converted to the mature, DNA-containing head. During maturation, all but one of the prohead proteins are proteolytically processed by a phage-coded protease which is formed by autocatalytic cleavage of the product of gene 21 (gp21). Protease gp21 has been tentatively located in the center of the prohead core.	+	[https://www.uniprot.org/uniprot/PCPP_BPT4 PCPP_BPT4] The pathway of bacteriophage T4 head assembly begins with the formation of a prohead bound to the bacterial cell membrane which is later converted to the mature, DNA-containing head. During maturation, all but one of the prohead proteins are proteolytically processed by a phage-coded protease which is formed by autocatalytic cleavage of the product of gene 21 (gp21). Protease gp21 has been tentatively located in the center of the prohead core.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpt4~~]]	+	[[Category: Escherichia virus T4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fokine, A]]	+	[[Category: Fokine A]]
-	[[Category: Rossmann~~, M G]]~~	+	[[Category: Rossmann MG]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phage t4]]~~	+
-	~~[[Category: Prohead]]~~	+
-	~~[[Category: Protease pentamer~~]]	+

OCA at 16:22, 11 December 2019

OCA — Wed, 11 Dec 2019 16:22:07 GMT

←Older revision		Revision as of 16:22, 11 December 2019
Line 1:		Line 1:

	==Structure of the bacteriophage T4 capsid assembly protease, gp21.==		==Structure of the bacteriophage T4 capsid assembly protease, gp21.==
-	<StructureSection load='5jbl' size='340' side='right' caption='[[5jbl]], [[Resolution\|resolution]] 1.94Å' scene=''>	+	<StructureSection load='5jbl' size='340' side='right'caption='[[5jbl]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5jbl]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JBL FirstGlance]. <br>		<table><tr><td colspan='2'>[[5jbl]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JBL FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Bpt4]]		[[Category: Bpt4]]
		+	[[Category: Large Structures]]
	[[Category: Fokine, A]]		[[Category: Fokine, A]]
	[[Category: Rossmann, M G]]		[[Category: Rossmann, M G]]

OCA at 17:53, 15 November 2017

OCA — Wed, 15 Nov 2017 17:53:18 GMT

←Older revision		Revision as of 17:53, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5jbl' size='340' side='right' caption='[[5jbl]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='5jbl' size='340' side='right' caption='[[5jbl]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jbl]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JBL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jbl]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JBL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jbl OCA], [http://pdbe.org/5jbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jbl RCSB], [http://www.ebi.ac.uk/pdbsum/5jbl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jbl ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jbl OCA], [http://pdbe.org/5jbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jbl RCSB], [http://www.ebi.ac.uk/pdbsum/5jbl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jbl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bpt4]]
	[[Category: Fokine, A]]		[[Category: Fokine, A]]
	[[Category: Rossmann, M G]]		[[Category: Rossmann, M G]]

OCA at 19:40, 9 December 2016

OCA — Fri, 09 Dec 2016 19:40:19 GMT

←Older revision		Revision as of 19:40, 9 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5jbl is ~~ON HOLD until Paper~~ Publication	+	==Structure of the bacteriophage T4 capsid assembly protease, gp21.==
		+	<StructureSection load='5jbl' size='340' side='right' caption='[[5jbl]], [[Resolution\|resolution]] 1.94Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5jbl]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JBL FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jbl OCA], [http://pdbe.org/5jbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jbl RCSB], [http://www.ebi.ac.uk/pdbsum/5jbl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jbl ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PCPP_BPT4 PCPP_BPT4]] The pathway of bacteriophage T4 head assembly begins with the formation of a prohead bound to the bacterial cell membrane which is later converted to the mature, DNA-containing head. During maturation, all but one of the prohead proteins are proteolytically processed by a phage-coded protease which is formed by autocatalytic cleavage of the product of gene 21 (gp21). Protease gp21 has been tentatively located in the center of the prohead core.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many large viruses, including tailed dsDNA bacteriophages and herpesviruses, assemble their capsids via formation of precursors, called procapsids or proheads. The prohead has an internal core, made of scaffolding proteins, and an outer shell, formed by the major capsid protein. The prohead usually contains a protease, which is activated during capsid maturation to destroy the inner core and liberate space for the genome. Here, we report a 2.0 A resolution structure of the pentameric procapsid protease of bacteriophage T4, gene product (gp)21. The structure corresponds to the enzyme's pre-active state in which its N-terminal region blocks the catalytic center, demonstrating that the activation mechanism involves self-cleavage of nine N-terminal residues. We describe similarities and differences between T4 gp21 and related herpesvirus proteases. We found that gp21 and the herpesvirus proteases have similarity with proteins forming the tubes of phage tails and bacterial type VI secretion systems, suggesting their common evolutionary origin.

-	~~Authors: Fokine~~, A., ~~Rossmann~~, M.G.	+	Common Evolutionary Origin of Procapsid Proteases, Phage Tail Tubes, and Tubes of Bacterial Type VI Secretion Systems.,Fokine A, Rossmann MG Structure. 2016 Nov 1;24(11):1928-1935. doi: 10.1016/j.str.2016.08.013. Epub 2016, Sep 22. PMID:27667692<ref>PMID:27667692</ref>

-	~~Description: Structure~~ of the ~~bacteriophage T4 capsid assembly protease, gp21~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5jbl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fokine, A]]		[[Category: Fokine, A]]
-	[[Category: Rossmann, M.G]]	+	[[Category: Rossmann, M G]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phage t4]]
		+	[[Category: Prohead]]
		+	[[Category: Protease pentamer]]

OCA: Protected "5jbl" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:48:36 GMT

Protected "5jbl" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:48, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5jbl is ON HOLD until Paper Publication Authors: Fokine, A., Rossmann, M.G. Description: Structure of the bacteriophage T4 capsid assembly protease...

OCA — Wed, 11 May 2016 01:48:35 GMT

New page: '''Unreleased structure''' The entry 5jbl is ON HOLD until Paper Publication Authors: Fokine, A., Rossmann, M.G. Description: Structure of the bacteriophage T4 capsid assembly protease...

New page

'''Unreleased structure'''

The entry 5jbl is ON HOLD until Paper Publication

Authors: Fokine, A., Rossmann, M.G.

Description: Structure of the bacteriophage T4 capsid assembly protease, gp21.
[[Category: Unreleased Structures]]
[[Category: Fokine, A]]
[[Category: Rossmann, M.G]]