5jd9 - Revision history

OCA at 12:36, 6 March 2024

OCA — Wed, 06 Mar 2024 12:36:56 GMT

←Older revision		Revision as of 12:36, 6 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q739M5_BACC1 Q739M5_BACC1]		[https://www.uniprot.org/uniprot/Q739M5_BACC1 Q739M5_BACC1]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The modification of proteins by phosphorylation occurs in all life forms and is catalyzed by a large superfamily of enzymes known as protein kinases. We recently discovered a family of secretory pathway kinases that phosphorylate extracellular proteins. One member, family with sequence similarity 20C (Fam20C), is the physiological Golgi casein kinase. While examining distantly related protein sequences, we observed low levels of identity between the spore coat protein H (CotH), and the Fam20C-related secretory pathway kinases. CotH is a component of the spore in many bacterial and eukaryotic species, and is required for efficient germination of spores in Bacillus subtilis; however, the mechanism by which CotH affects germination is unclear. Here, we show that CotH is a protein kinase. The crystal structure of CotH reveals an atypical protein kinase-like fold with a unique mode of ATP binding. Examination of the genes neighboring cotH in B. subtilis led us to identify two spore coat proteins, CotB and CotG, as CotH substrates. Furthermore, we show that CotH-dependent phosphorylation of CotB and CotG is required for the efficient germination of B. subtilis spores. Collectively, our results define a family of atypical protein kinases and reveal an unexpected role for protein phosphorylation in spore biology.
-
-	Phosphorylation of spore coat proteins by a family of atypical protein kinases.,Nguyen KB, Sreelatha A, Durrant ES, Lopez-Garrido J, Muszewska A, Dudkiewicz M, Grynberg M, Yee S, Pogliano K, Tomchick DR, Pawlowski K, Dixon JE, Tagliabracci VS Proc Natl Acad Sci U S A. 2016 May 16. pii: 201605917. PMID:27185916<ref>PMID:27185916</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5jd9" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:59, 6 September 2023

OCA — Wed, 06 Sep 2023 10:59:55 GMT

←Older revision		Revision as of 10:59, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='5jd9' size='340' side='right'caption='[[5jd9]], [[Resolution\|resolution]] 1.63Å' scene=''>		<StructureSection load='5jd9' size='340' side='right'caption='[[5jd9]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jd9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacc1 Bacc1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JD9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5JD9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jd9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_10987 Bacillus cereus ATCC 10987]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JD9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1,2~~-~~ETHANEDIOL'>EDO</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.63Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~cotH, BCE_2115 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=222523 BACC1])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [~~http~~://pdbe.org/5jd9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jd9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jd9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [https://pdbe.org/5jd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [https://www.ebi.ac.uk/pdbsum/5jd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jd9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q739M5_BACC1 Q739M5_BACC1]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacc1~~]]	+	[[Category: Bacillus cereus ATCC 10987]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Sreelatha, A]]	+	[[Category: Sreelatha A]]
-	[[Category: Tagliabracci~~, V S~~]]	+	[[Category: Tagliabracci VS]]
-	[[Category: Tomchick~~, D R]]~~	+	[[Category: Tomchick DR]]
-	~~[[Category: Atypical kinase fold]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 07:43, 10 June 2020

OCA — Wed, 10 Jun 2020 07:43:44 GMT

←Older revision		Revision as of 07:43, 10 June 2020
Line 1:		Line 1:

	==Bacillus cereus CotH kinase==		==Bacillus cereus CotH kinase==
-	<StructureSection load='5jd9' size='340' side='right' caption='[[5jd9]], [[Resolution\|resolution]] 1.63Å' scene=''>	+	<StructureSection load='5jd9' size='340' side='right'caption='[[5jd9]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jd9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JD9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JD9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jd9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacc1 Bacc1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JD9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JD9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [http://pdbe.org/5jd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jd9 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cotH, BCE_2115 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=222523 BACC1])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [http://pdbe.org/5jd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jd9 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacc1]]
		+	[[Category: Large Structures]]
	[[Category: Sreelatha, A]]		[[Category: Sreelatha, A]]
	[[Category: Tagliabracci, V S]]		[[Category: Tagliabracci, V S]]

OCA at 17:49, 12 July 2016

OCA — Tue, 12 Jul 2016 17:49:54 GMT

←Older revision		Revision as of 17:49, 12 July 2016
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[5jd9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JD9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5jd9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JD9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [http://pdbe.org/5jd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jd9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [http://pdbe.org/5jd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jd9 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:44, 20 June 2016

OCA — Mon, 20 Jun 2016 07:44:28 GMT

←Older revision		Revision as of 07:44, 20 June 2016
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [http://pdbe.org/5jd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jd9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [http://pdbe.org/5jd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jd9 PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The modification of proteins by phosphorylation occurs in all life forms and is catalyzed by a large superfamily of enzymes known as protein kinases. We recently discovered a family of secretory pathway kinases that phosphorylate extracellular proteins. One member, family with sequence similarity 20C (Fam20C), is the physiological Golgi casein kinase. While examining distantly related protein sequences, we observed low levels of identity between the spore coat protein H (CotH), and the Fam20C-related secretory pathway kinases. CotH is a component of the spore in many bacterial and eukaryotic species, and is required for efficient germination of spores in Bacillus subtilis; however, the mechanism by which CotH affects germination is unclear. Here, we show that CotH is a protein kinase. The crystal structure of CotH reveals an atypical protein kinase-like fold with a unique mode of ATP binding. Examination of the genes neighboring cotH in B. subtilis led us to identify two spore coat proteins, CotB and CotG, as CotH substrates. Furthermore, we show that CotH-dependent phosphorylation of CotB and CotG is required for the efficient germination of B. subtilis spores. Collectively, our results define a family of atypical protein kinases and reveal an unexpected role for protein phosphorylation in spore biology.
		+
		+	Phosphorylation of spore coat proteins by a family of atypical protein kinases.,Nguyen KB, Sreelatha A, Durrant ES, Lopez-Garrido J, Muszewska A, Dudkiewicz M, Grynberg M, Yee S, Pogliano K, Tomchick DR, Pawlowski K, Dixon JE, Tagliabracci VS Proc Natl Acad Sci U S A. 2016 May 16. pii: 201605917. PMID:27185916<ref>PMID:27185916</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5jd9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:58, 1 June 2016

OCA — Wed, 01 Jun 2016 15:58:07 GMT

←Older revision		Revision as of 15:58, 1 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5jd9 ~~is ON HOLD until Paper Publication~~	+	==Bacillus cereus CotH kinase==
-		+	<StructureSection load='5jd9' size='340' side='right' caption='[[5jd9]], [[Resolution\|resolution]] 1.63Å' scene=''>
-	~~Authors~~: ~~Tomchick, D~~.R.~~, Tagliabracci, V~~.S.~~, Sreelatha, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5jd9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JD9 FirstGlance]. <br>
-	~~Description: Bacillus cereus CotH kinase~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jd9 OCA], [http://pdbe.org/5jd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jd9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jd9 PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Tomchick~~, D.R]]	+	</table>
-	[[~~Category~~: ~~Tagliabracci~~, V.S]]	+	__TOC__
		+	</StructureSection>
	[[Category: Sreelatha, A]]		[[Category: Sreelatha, A]]
		+	[[Category: Tagliabracci, V S]]
		+	[[Category: Tomchick, D R]]
		+	[[Category: Atypical kinase fold]]
		+	[[Category: Structural protein]]

OCA at 15:18, 15 May 2016

OCA — Sun, 15 May 2016 15:18:21 GMT

←Older revision		Revision as of 15:18, 15 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5jd9 is ON HOLD	+	The entry 5jd9 is ON HOLD until Paper Publication

	Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A.		Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A.

OCA: Protected "5jd9" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:53:05 GMT

Protected "5jd9" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:53, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5jd9 is ON HOLD Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A. Description: Bacillus cereus CotH kinase [[Category: Unreleased Structur...

OCA — Wed, 11 May 2016 01:53:04 GMT

New page: '''Unreleased structure''' The entry 5jd9 is ON HOLD Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A. Description: Bacillus cereus CotH kinase [[Category: Unreleased Structur...

New page

'''Unreleased structure'''

The entry 5jd9 is ON HOLD

Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A.

Description: Bacillus cereus CotH kinase
[[Category: Unreleased Structures]]
[[Category: Tomchick, D.R]]
[[Category: Tagliabracci, V.S]]
[[Category: Sreelatha, A]]