5jfz - Revision history

OCA at 11:02, 6 September 2023

OCA — Wed, 06 Sep 2023 11:02:44 GMT

←Older revision		Revision as of 11:02, 6 September 2023
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	<StructureSection load='5jfz' size='340' side='right'caption='[[5jfz]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5jfz' size='340' side='right'caption='[[5jfz]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jfz]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JFZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5JFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jfz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JFZ FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~fic~~, ~~b3361, JW3324 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI~~]~~), yhfG, b3362, JW3325 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83333 ECOLI])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jfz OCA], [~~http~~://pdbe.org/5jfz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jfz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jfz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jfz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jfz OCA], [https://pdbe.org/5jfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jfz RCSB], [https://www.ebi.ac.uk/pdbsum/5jfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jfz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FIC_ECOLI FIC_ECOLI]] Probable adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (By similarity). Involved in cell filamentation induced by cyclic AMP. May have some role in cell division.	+	[https://www.uniprot.org/uniprot/FIC_ECOLI FIC_ECOLI] Probable adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (By similarity). Involved in cell filamentation induced by cyclic AMP. May have some role in cell division.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Schirmer, T]]	+	[[Category: Schirmer T]]
-	[[Category: Stanger~~, F V]]~~	+	[[Category: Stanger FV]]
-	~~[[Category: Class i fic protein]]~~	+
-	~~[[Category: Fic domain]]~~	+
-	~~[[Category: Fic-1]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:47, 10 June 2020

OCA — Wed, 10 Jun 2020 07:47:34 GMT

←Older revision		Revision as of 07:47, 10 June 2020
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	==E. coli EcFicT in complex with EcFicA mutant E28G==		==E. coli EcFicT in complex with EcFicA mutant E28G==
-	<StructureSection load='5jfz' size='340' side='right' caption='[[5jfz]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5jfz' size='340' side='right'caption='[[5jfz]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jfz]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JFZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jfz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JFZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JFZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jfz OCA], [http://pdbe.org/5jfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jfz RCSB], [http://www.ebi.ac.uk/pdbsum/5jfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jfz ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fic, b3361, JW3324 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), yhfG, b3362, JW3325 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jfz OCA], [http://pdbe.org/5jfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jfz RCSB], [http://www.ebi.ac.uk/pdbsum/5jfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jfz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Schirmer, T]]		[[Category: Schirmer, T]]
	[[Category: Stanger, F V]]		[[Category: Stanger, F V]]

OCA at 21:30, 5 October 2016

OCA — Wed, 05 Oct 2016 21:30:42 GMT

←Older revision		Revision as of 21:30, 5 October 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5jfz is ~~ON HOLD~~	+	==E. coli EcFicT in complex with EcFicA mutant E28G==
		+	<StructureSection load='5jfz' size='340' side='right' caption='[[5jfz]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5jfz]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JFZ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jfz OCA], [http://pdbe.org/5jfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jfz RCSB], [http://www.ebi.ac.uk/pdbsum/5jfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jfz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FIC_ECOLI FIC_ECOLI]] Probable adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (By similarity). Involved in cell filamentation induced by cyclic AMP. May have some role in cell division.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	FIC domain proteins mediate post-translational modifications of target proteins, which typically results in their inactivation. Depending on the conservation of crucial active site residues, the FIC fold serves as structural scaffold for various enzymatic activities, mostly target adenylylation. The founding member of the vast Fic protein family, EcFicT, was identified in Escherichia coli some time ago. The G55R point mutant of EcFicT displays the "filamentation induced by cAMP" (Fic) phenotype at high 3',5'-cyclic adenosine monophosphate (cAMP) concentrations and elevated temperature, but the underlying molecular mechanism and any putative biochemical activity of EcFicT have remained unknown. EcFicT belongs to class I Fic toxin proteins that are encoded together with a small inhibitory protein (antitoxin), named EcFicA in E. coli. Here, we report the crystal structures of two mutant EcFicT/EcFicA complexes (EcFicTG55RA and EcFicTAE28G) both showing close resemblance with the structure of the AMP-transferase VbhT from Bartonella schoenbuchensis in complex with its cognate antitoxin VbhA. However, crucial differences in the active site of EcFicT compared to VbhT and other AMP-transferases rationalize the lack of evidence for adenylylation activity. Comprehensive bioinformatic analysis suggests that EcFicT has evolved from canonical AMP-transferases and has acquired a conserved binding site for a yet to be discovered novel substrate. The G55R mutation has no effect on structure or thermal stability of EcFicT, such that the molecular basis for its associated Fic phenotype remains elusive. We anticipate that this structure will inspire further bioinformatic and experimental analyses in order to characterize the enzymatic activity of EcFicT and help revealing its physiological role.

-	~~Authors~~:	+	Crystal Structure of the Escherichia coli Fic Toxin-Like Protein in Complex with Its Cognate Antitoxin.,Stanger FV, Harms A, Dehio C, Schirmer T PLoS One. 2016 Sep 22;11(9):e0163654. doi: 10.1371/journal.pone.0163654., eCollection 2016. PMID:27657533<ref>PMID:27657533</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5jfz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Schirmer, T]]
		+	[[Category: Stanger, F V]]
		+	[[Category: Class i fic protein]]
		+	[[Category: Fic domain]]
		+	[[Category: Fic-1]]
		+	[[Category: Transferase]]

OCA at 15:22, 15 May 2016

OCA — Sun, 15 May 2016 15:22:43 GMT

←Older revision		Revision as of 15:22, 15 May 2016
Line 3:		Line 3:
	The entry 5jfz is ON HOLD		The entry 5jfz is ON HOLD

-	Authors: ~~Stanger, F.V., Schirmer, T.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Schirmer, T]]
-	[[Category: Stanger, F.V]]

OCA: Protected "5jfz" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 01:59:55 GMT

Protected "5jfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:59, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5jfz is ON HOLD Authors: Stanger, F.V., Schirmer, T. Description: Category: Unreleased Structures Category: Schirmer, T [[Category: Stange...

OCA — Wed, 11 May 2016 01:59:54 GMT

New page: '''Unreleased structure''' The entry 5jfz is ON HOLD Authors: Stanger, F.V., Schirmer, T. Description: Category: Unreleased Structures Category: Schirmer, T [[Category: Stange...

New page

'''Unreleased structure'''

The entry 5jfz is ON HOLD

Authors: Stanger, F.V., Schirmer, T.

Description:
[[Category: Unreleased Structures]]
[[Category: Schirmer, T]]
[[Category: Stanger, F.V]]