5jg4 - Revision history

OCA at 12:37, 6 March 2024

OCA — Wed, 06 Mar 2024 12:37:26 GMT

←Older revision		Revision as of 12:37, 6 March 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [https://pdbe.org/5jg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [https://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [https://pdbe.org/5jg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [https://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Legionella pneumophila is a causative agent of a severe pneumonia, known as Legionnaires' disease. Legionella's pathogenicity is mediated by specific virulence factors, called bacterial effectors, which are injected into the invaded host cell by the bacterial Type IV Secretion System. Bacterial effectors are involved in complex interactions with the components of the host cell immune and signaling pathways, that eventually lead to bacterial survival and replication inside the mammalian cell. Structural and functional studies of bacterial effectors are therefore crucial for elucidating the mechanisms of Legionella virulence. Here we describe the crystal structure of the LpiR1 (Lpg0634) effector protein and investigate the effects of its overexpression in mammalian cells. LpiR1 is an alpha-helical protein, that consists of two similar domains aligned in an antiparallel fashion. The hydrophilic cleft between the domains might serve as a binding site for a potential host cell interaction partner. LpiR1 binds phosphate group at a conserved site and is stabilized by Mn2+, Ca2+ or Mg2+ ions. When overexpressed in mammalian cells, a GFP-LpiR1 fusion protein is localized in the cytoplasm. Intracellular signaling antibody array analysis revealed small changes in the phosphorylation state of several components of the Akt signaling pathway in HEK293T cells overexpressing LpiR1.
-
-	Structural and functional investigations of the effector protein LpiR1 from Legionella pneumophila.,Beyrakhova KA, van Straaten K, Li L, Boniecki MT, Anderson DH, Cygler M J Biol Chem. 2016 May 17. pii: jbc.M115.708701. PMID:27226543<ref>PMID:27226543</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5jg4" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:02, 6 September 2023

OCA — Wed, 06 Sep 2023 11:02:53 GMT

←Older revision		Revision as of 11:02, 6 September 2023
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	<StructureSection load='5jg4' size='340' side='right'caption='[[5jg4]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5jg4' size='340' side='right'caption='[[5jg4]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jg4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_33152 Atcc 33152~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JG4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5JG4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JG4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5fia\|5fia]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<~~tr id~~='~~gene~~'><~~td class="sblockLbl"~~><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">lpymg_00644 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [https://pdbe.org/5jg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [https://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [~~http~~://pdbe.org/5jg4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 33152]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Beyrakhova, K]]
-	[[Category: Cygler, M]]
-	[[Category: Straaten, K van]]
-	[[Category: Bacterial effector]]
	[[Category: Legionella pneumophila]]		[[Category: Legionella pneumophila]]
-	[[Category: ~~Phosphate binding~~]]	+	[[Category: Beyrakhova K]]
-	[[Category: ~~Signaling protein~~]]	+	[[Category: Cygler M]]
		+	[[Category: Van Straaten K]]

OCA at 07:47, 10 June 2020

OCA — Wed, 10 Jun 2020 07:47:39 GMT

←Older revision		Revision as of 07:47, 10 June 2020
Line 1:		Line 1:

	==Structure of the effector protein LpiR1 (Lpg0634) from Legionella pneumophila==		==Structure of the effector protein LpiR1 (Lpg0634) from Legionella pneumophila==
-	<StructureSection load='5jg4' size='340' side='right' caption='[[5jg4]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5jg4' size='340' side='right'caption='[[5jg4]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jg4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JG4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JG4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jg4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JG4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JG4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fia\|5fia]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fia\|5fia]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [http://pdbe.org/5jg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpymg_00644 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [http://pdbe.org/5jg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33152]]
		+	[[Category: Large Structures]]
	[[Category: Beyrakhova, K]]		[[Category: Beyrakhova, K]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]

OCA at 20:47, 3 August 2016

OCA — Wed, 03 Aug 2016 20:47:35 GMT

←Older revision		Revision as of 20:47, 3 August 2016
Line 6:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fia\|5fia]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fia\|5fia]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [http://pdbe.org/5jg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jg4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [http://pdbe.org/5jg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jg4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jg4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:11, 20 June 2016

OCA — Mon, 20 Jun 2016 08:11:44 GMT

←Older revision		Revision as of 08:11, 20 June 2016
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [http://pdbe.org/5jg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jg4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [http://pdbe.org/5jg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jg4 PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Legionella pneumophila is a causative agent of a severe pneumonia, known as Legionnaires' disease. Legionella's pathogenicity is mediated by specific virulence factors, called bacterial effectors, which are injected into the invaded host cell by the bacterial Type IV Secretion System. Bacterial effectors are involved in complex interactions with the components of the host cell immune and signaling pathways, that eventually lead to bacterial survival and replication inside the mammalian cell. Structural and functional studies of bacterial effectors are therefore crucial for elucidating the mechanisms of Legionella virulence. Here we describe the crystal structure of the LpiR1 (Lpg0634) effector protein and investigate the effects of its overexpression in mammalian cells. LpiR1 is an alpha-helical protein, that consists of two similar domains aligned in an antiparallel fashion. The hydrophilic cleft between the domains might serve as a binding site for a potential host cell interaction partner. LpiR1 binds phosphate group at a conserved site and is stabilized by Mn2+, Ca2+ or Mg2+ ions. When overexpressed in mammalian cells, a GFP-LpiR1 fusion protein is localized in the cytoplasm. Intracellular signaling antibody array analysis revealed small changes in the phosphorylation state of several components of the Akt signaling pathway in HEK293T cells overexpressing LpiR1.
		+
		+	Structural and functional investigations of the effector protein LpiR1 from Legionella pneumophila.,Beyrakhova KA, van Straaten K, Li L, Boniecki MT, Anderson DH, Cygler M J Biol Chem. 2016 May 17. pii: jbc.M115.708701. PMID:27226543<ref>PMID:27226543</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5jg4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA: Protected "5jg4" [edit=sysop:move=sysop]

OCA — Fri, 13 May 2016 19:49:48 GMT

Protected "5jg4" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:49, 13 May 2016

OCA: New page: ==Structure of the effector protein LpiR1 (Lpg0634) from Legionella pneumophila== == Structural highlights ==
<table><tr><td colspan='2'>[[5jg4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JG4 OCA]. For a guided tour on the structure components use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JG4 FirstGlance]. 
</td></tr><tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='related'><td class="sblockLbl">[[Related_structure|Related:]]</td><td class="sblockDat">[[5fia|5fia]]</td></tr>
<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jg4 OCA], [http://pdbe.org/5jg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jg4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jg4 PDBsum]</td></tr>
</table>
TOC
</StructureSection>
[[Category: Beyrakhova, K]]
[[Category: Cygler, M]]
[[Category: Straaten, K van]]
[[Category: Bacterial effector]]
[[Category: Legionella pneumophila]]
[[Category: Phosphate binding]]
[[Category: Signaling protein]]