5jgc - Revision history

OCA at 12:37, 6 March 2024

OCA — Wed, 06 Mar 2024 12:37:27 GMT

←Older revision		Revision as of 12:37, 6 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/TPR1_ORYSJ TPR1_ORYSJ] Probable downstream regulator of strigolactones signaling.[UniProtKB:Q0J7U6]		[https://www.uniprot.org/uniprot/TPR1_ORYSJ TPR1_ORYSJ] Probable downstream regulator of strigolactones signaling.[UniProtKB:Q0J7U6]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	TOPLESS are tetrameric plant corepressors of the conserved Tup1/Groucho/TLE (transducin-like enhancer of split) family. We show that they interact through their TOPLESS domains (TPDs) with two functionally important ethylene response factor-associated amphiphilic repression (EAR) motifs of the rice strigolactone signaling repressor D53: the universally conserved EAR-3 and the monocot-specific EAR-2. We present the crystal structure of the monocot-specific EAR-2 peptide in complex with the TOPLESS-related protein 2 (TPR2) TPD, in which the EAR-2 motif binds the same TPD groove as jasmonate and auxin signaling repressors but makes additional contacts with a second TPD site to mediate TPD tetramer-tetramer interaction. We validated the functional relevance of the two TPD binding sites in reporter gene assays and in transgenic rice and demonstrate that EAR-2 binding induces TPD oligomerization. Moreover, we demonstrate that the TPD directly binds nucleosomes and the tails of histones H3 and H4. Higher-order assembly of TPD complexes induced by EAR-2 binding markedly stabilizes the nucleosome-TPD interaction. These results establish a new TPD-repressor binding mode that promotes TPD oligomerization and TPD-nucleosome interaction, thus illustrating the initial assembly of a repressor-corepressor-nucleosome complex.
-
-	A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex.,Ma H, Duan J, Ke J, He Y, Gu X, Xu TH, Yu H, Wang Y, Brunzelle JS, Jiang Y, Rothbart SB, Xu HE, Li J, Melcher K Sci Adv. 2017 Jun 2;3(6):e1601217. doi: 10.1126/sciadv.1601217. eCollection 2017 , Jun. PMID:28630893<ref>PMID:28630893</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5jgc" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:03, 6 September 2023

OCA — Wed, 06 Sep 2023 11:03:07 GMT

←Older revision		Revision as of 11:03, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='5jgc' size='340' side='right'caption='[[5jgc]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='5jgc' size='340' side='right'caption='[[5jgc]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jgc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Orysa Orysa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jgc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JGC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.08Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5j9k\|5j9k]]~~, [[~~5ja5~~\|~~5ja5]], [[5jhp\|5jhp~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TPR1, ASPR2, TPR2, Os01g0254100, LOC_Os01g15020, OsJ_01134, OSNPB_010254100, P0705D01.10-1 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4530 ORYSA])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgc OCA], [https://pdbe.org/5jgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jgc RCSB], [https://www.ebi.ac.uk/pdbsum/5jgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgc ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgc OCA], [~~http~~://pdbe.org/5jgc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jgc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jgc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgc ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TPR1_ORYSJ TPR1_ORYSJ]] Probable downstream regulator of strigolactones signaling.[UniProtKB:Q0J7U6]	+	[https://www.uniprot.org/uniprot/TPR1_ORYSJ TPR1_ORYSJ] Probable downstream regulator of strigolactones signaling.[UniProtKB:Q0J7U6]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Orysa~~]]	+	[[Category: Oryza sativa]]
-	[[Category: Brunzelle~~, J S~~]]	+	[[Category: Brunzelle JS]]
-	[[Category: Gu, X]]	+	[[Category: Gu X]]
-	[[Category: Ke, J]]	+	[[Category: Ke J]]
-	[[Category: Ma, H]]	+	[[Category: Ma H]]
-	[[Category: Melcher, K]]	+	[[Category: Melcher K]]
-	[[Category: Xu~~, H E]]~~	+	[[Category: Xu HE]]
-	~~[[Category: Alpha-helical structure]]~~	+
-	~~[[Category: Tetramer]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription repression]]~~	+
-	~~[[Category: Transcriptional corepressor topless]]~~	+
-	~~[[Category: Transcriptional repressor d53~~]]	+

OCA at 12:01, 25 December 2019

OCA — Wed, 25 Dec 2019 12:01:20 GMT

←Older revision		Revision as of 12:01, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A, L130A, L179A and I195A mutant==		==Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A, L130A, L179A and I195A mutant==
-	<StructureSection load='5jgc' size='340' side='right' caption='[[5jgc]], [[Resolution\|resolution]] 2.08Å' scene=''>	+	<StructureSection load='5jgc' size='340' side='right'caption='[[5jgc]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jgc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jgc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Orysa Orysa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JGC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5j9k\|5j9k]], [[5ja5\|5ja5]], [[5jhp\|5jhp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5j9k\|5j9k]], [[5ja5\|5ja5]], [[5jhp\|5jhp]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPR1, ASPR2, TPR2, Os01g0254100, LOC_Os01g15020, OsJ_01134, OSNPB_010254100, P0705D01.10-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4530 ORYSA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgc OCA], [http://pdbe.org/5jgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jgc RCSB], [http://www.ebi.ac.uk/pdbsum/5jgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgc OCA], [http://pdbe.org/5jgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jgc RCSB], [http://www.ebi.ac.uk/pdbsum/5jgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgc ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Orysa]]
	[[Category: Brunzelle, J S]]		[[Category: Brunzelle, J S]]
	[[Category: Gu, X]]		[[Category: Gu, X]]

OCA at 10:28, 27 September 2017

OCA — Wed, 27 Sep 2017 10:28:16 GMT

←Older revision		Revision as of 10:28, 27 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5jgc is ~~ON HOLD until Oct 18 2018~~	+	==Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A, L130A, L179A and I195A mutant==
		+	<StructureSection load='5jgc' size='340' side='right' caption='[[5jgc]], [[Resolution\|resolution]] 2.08Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5jgc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JGC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5j9k\|5j9k]], [[5ja5\|5ja5]], [[5jhp\|5jhp]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgc OCA], [http://pdbe.org/5jgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jgc RCSB], [http://www.ebi.ac.uk/pdbsum/5jgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgc ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TPR1_ORYSJ TPR1_ORYSJ]] Probable downstream regulator of strigolactones signaling.[UniProtKB:Q0J7U6]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	TOPLESS are tetrameric plant corepressors of the conserved Tup1/Groucho/TLE (transducin-like enhancer of split) family. We show that they interact through their TOPLESS domains (TPDs) with two functionally important ethylene response factor-associated amphiphilic repression (EAR) motifs of the rice strigolactone signaling repressor D53: the universally conserved EAR-3 and the monocot-specific EAR-2. We present the crystal structure of the monocot-specific EAR-2 peptide in complex with the TOPLESS-related protein 2 (TPR2) TPD, in which the EAR-2 motif binds the same TPD groove as jasmonate and auxin signaling repressors but makes additional contacts with a second TPD site to mediate TPD tetramer-tetramer interaction. We validated the functional relevance of the two TPD binding sites in reporter gene assays and in transgenic rice and demonstrate that EAR-2 binding induces TPD oligomerization. Moreover, we demonstrate that the TPD directly binds nucleosomes and the tails of histones H3 and H4. Higher-order assembly of TPD complexes induced by EAR-2 binding markedly stabilizes the nucleosome-TPD interaction. These results establish a new TPD-repressor binding mode that promotes TPD oligomerization and TPD-nucleosome interaction, thus illustrating the initial assembly of a repressor-corepressor-nucleosome complex.

-	~~Authors: Ke, J~~., Ma, H., Gu, X., Brunzelle, ~~J.S.~~, Xu, ~~H.E.~~, Melcher, K.	+	A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex.,Ma H, Duan J, Ke J, He Y, Gu X, Xu TH, Yu H, Wang Y, Brunzelle JS, Jiang Y, Rothbart SB, Xu HE, Li J, Melcher K Sci Adv. 2017 Jun 2;3(6):e1601217. doi: 10.1126/sciadv.1601217. eCollection 2017 , Jun. PMID:28630893<ref>PMID:28630893</ref>

-	~~Description: Crystal structure~~ of the ~~rice Topless related protein 2 (TPR2) N~~-~~terminal topless domain (1~~-~~209) L111A, L130A, L179A and I195A mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Xu, H.E]]~~	+	<div class="pdbe-citations 5jgc" style="background-color:#fffaf0;"></div>
-	~~[[Category: Ma, H]]~~	+	== References ==
-	~~[[Category: Melcher, K]]~~	+	<references/>
-	[[Category: Brunzelle, J.S]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Brunzelle, J S]]
	[[Category: Gu, X]]		[[Category: Gu, X]]
	[[Category: Ke, J]]		[[Category: Ke, J]]
		+	[[Category: Ma, H]]
		+	[[Category: Melcher, K]]
		+	[[Category: Xu, H E]]
		+	[[Category: Alpha-helical structure]]
		+	[[Category: Tetramer]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription repression]]
		+	[[Category: Transcriptional corepressor topless]]
		+	[[Category: Transcriptional repressor d53]]

OCA at 15:01, 15 March 2017

OCA — Wed, 15 Mar 2017 15:01:09 GMT

←Older revision		Revision as of 15:01, 15 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5jgc is ON HOLD until ~~Paper Publication~~	+	The entry 5jgc is ON HOLD until Oct 18 2018

	Authors: Ke, J., Ma, H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K.		Authors: Ke, J., Ma, H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K.

OCA at 09:52, 9 March 2017

OCA — Thu, 09 Mar 2017 09:52:33 GMT

←Older revision		Revision as of 09:52, 9 March 2017
Line 10:		Line 10:
	[[Category: Ma, H]]		[[Category: Ma, H]]
	[[Category: Melcher, K]]		[[Category: Melcher, K]]
-	[[Category: Gu, X]]
	[[Category: Brunzelle, J.S]]		[[Category: Brunzelle, J.S]]
		+	[[Category: Gu, X]]
	[[Category: Ke, J]]		[[Category: Ke, J]]

OCA at 20:18, 23 June 2016

OCA — Thu, 23 Jun 2016 20:18:27 GMT

←Older revision		Revision as of 20:18, 23 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5jgc is ON HOLD	+	The entry 5jgc is ON HOLD until Paper Publication

	Authors: Ke, J., Ma, H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K.		Authors: Ke, J., Ma, H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K.

OCA at 13:23, 1 June 2016

OCA — Wed, 01 Jun 2016 13:23:48 GMT

←Older revision		Revision as of 13:23, 1 June 2016
Line 3:		Line 3:
	The entry 5jgc is ON HOLD		The entry 5jgc is ON HOLD

-	Authors: Ke, J., Ma., H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K.	+	Authors: Ke, J., Ma, H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K.

	Description: Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A, L130A, L179A and I195A mutant		Description: Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A, L130A, L179A and I195A mutant
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Xu, H.E]]		[[Category: Xu, H.E]]
		+	[[Category: Ma, H]]
	[[Category: Melcher, K]]		[[Category: Melcher, K]]
-	[[Category: H]]
	[[Category: Gu, X]]		[[Category: Gu, X]]
-	[[Category: Ma]]
	[[Category: Brunzelle, J.S]]		[[Category: Brunzelle, J.S]]
	[[Category: Ke, J]]		[[Category: Ke, J]]

OCA: Protected "5jgc" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 02:00:56 GMT

Protected "5jgc" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:00, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5jgc is ON HOLD Authors: Ke, J., Ma., H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K. Description: Crystal structure of the rice Topless related...

OCA — Wed, 11 May 2016 02:00:55 GMT

New page: '''Unreleased structure''' The entry 5jgc is ON HOLD Authors: Ke, J., Ma., H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K. Description: Crystal structure of the rice Topless related...

New page

'''Unreleased structure'''

The entry 5jgc is ON HOLD

Authors: Ke, J., Ma., H., Gu, X., Brunzelle, J.S., Xu, H.E., Melcher, K.

Description: Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A, L130A, L179A and I195A mutant
[[Category: Unreleased Structures]]
[[Category: Xu, H.E]]
[[Category: Melcher, K]]
[[Category: H]]
[[Category: Gu, X]]
[[Category: Ma]]
[[Category: Brunzelle, J.S]]
[[Category: Ke, J]]