5jgk - Revision history

OCA at 11:03, 6 September 2023

OCA — Wed, 06 Sep 2023 11:03:16 GMT

←Older revision		Revision as of 11:03, 6 September 2023
Line 1:		Line 1:

	==Crystal structure of GtmA in complex with SAH==		==Crystal structure of GtmA in complex with SAH==
-	<StructureSection load='5jgk' size='340' side='right' caption='[[5jgk]], [[Resolution\|resolution]] 1.33Å' scene=''>	+	<StructureSection load='5jgk' size='340' side='right'caption='[[5jgk]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jgk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aspergillus_fumigatus_z5~~ Aspergillus fumigatus z5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JGK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jgk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Z5 Aspergillus fumigatus Z5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JGK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SAH:S~~-~~ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.33Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Y699_02735 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=1437362 Aspergillus fumigatus Z5])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgk OCA], [~~http~~://pdbe.org/5jgk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jgk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jgk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgk OCA], [https://pdbe.org/5jgk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jgk RCSB], [https://www.ebi.ac.uk/pdbsum/5jgk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4X158_ASPFU Q4X158_ASPFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aspergillus fumigatus z5]]	+	[[Category: Aspergillus fumigatus Z5]]
-	[[Category: Blankenfeldt, W]]	+	[[Category: Large Structures]]
-	[[Category: Bock, T]]	+	[[Category: Blankenfeldt W]]
-	[[Category: Dolan~~, S K~~]]	+	[[Category: Bock T]]
-	[[Category: Dolye, S]]	+	[[Category: Dolan SK]]
-	[[Category: Hering, V]]	+	[[Category: Dolye S]]
-	[[Category: Jones~~, G W]]~~	+	[[Category: Hering V]]
-	~~[[Category: Aspergillus fumigatus]]~~	+	[[Category: Jones GW]]
-	~~[[Category: Gliotoxin]]~~	+
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Resistance]]~~	+
-	~~[[Category: S-adenosyl-l-homocysteine]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:41, 22 February 2018

OCA — Thu, 22 Feb 2018 07:41:28 GMT

←Older revision		Revision as of 07:41, 22 February 2018
Line 3:		Line 3:
	<StructureSection load='5jgk' size='340' side='right' caption='[[5jgk]], [[Resolution\|resolution]] 1.33Å' scene=''>		<StructureSection load='5jgk' size='340' side='right' caption='[[5jgk]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jgk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JGK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jgk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus_z5 Aspergillus fumigatus z5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JGK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Y699_02735 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1437362 Aspergillus fumigatus Z5])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgk OCA], [http://pdbe.org/5jgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jgk RCSB], [http://www.ebi.ac.uk/pdbsum/5jgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgk OCA], [http://pdbe.org/5jgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jgk RCSB], [http://www.ebi.ac.uk/pdbsum/5jgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgk ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspergillus fumigatus z5]]
	[[Category: Blankenfeldt, W]]		[[Category: Blankenfeldt, W]]
	[[Category: Bock, T]]		[[Category: Bock, T]]

OCA at 09:35, 11 March 2017

OCA — Sat, 11 Mar 2017 09:35:15 GMT

←Older revision		Revision as of 09:35, 11 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5jgk is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of GtmA in complex with SAH==
		+	<StructureSection load='5jgk' size='340' side='right' caption='[[5jgk]], [[Resolution\|resolution]] 1.33Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5jgk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JGK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jgk OCA], [http://pdbe.org/5jgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jgk RCSB], [http://www.ebi.ac.uk/pdbsum/5jgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jgk ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gliotoxin is an epipolythiodioxopiperazine (ETP) class toxin, contains a disulfide bridge that mediates its toxic effects via redox cycling and is produced by the opportunistic fungal pathogen Aspergillus fumigatus Self-resistance against gliotoxin is effected by the gliotoxin oxidase GliT, and attenuation of gliotoxin biosynthesis is catalysed by gliotoxin S-methyltransferase GtmA. Here we describe the X-ray crystal structures of GtmA-apo (1.66 A), GtmA complexed to S-adenosylhomocysteine (1.33 A) and GtmA complexed to S-adenosylmethionine (2.28 A), providing mechanistic insights into this important biotransformation. We further reveal that simultaneous elimination of the ability of A. fumigatus to dissipate highly reactive dithiol gliotoxin, via deletion of GliT and GtmA, results in the most significant hypersensitivity to exogenous gliotoxin observed to date. Indeed, quantitative proteomic analysis of DeltagliT::DeltagtmA reveals an uncontrolled over-activation of the gli-cluster upon gliotoxin exposure. The data presented herein reveal, for the first time, the extreme risk associated with intracellular dithiol gliotoxin biosynthesis-in the absence of an efficient dismutation capacity. Significantly, a previously concealed protective role for GtmA and functionality of ETP bis-thiomethylation as an ancestral protection strategy against dithiol compounds is now evident.

-	~~Authors: Dolan~~, ~~S.K~~., ~~Bock~~, T., Hering, V., Jones~~, G.W.~~, Blankenfeldt, W~~., Dolye~~, S.	+	Structural, mechanistic and functional insight into gliotoxin bis-thiomethylation in Aspergillus fumigatus.,Dolan SK, Bock T, Hering V, Owens RA, Jones GW, Blankenfeldt W, Doyle S Open Biol. 2017 Feb;7(2). pii: 160292. doi: 10.1098/rsob.160292. PMID:28179499<ref>PMID:28179499</ref>

-	~~Description: Crystal structure~~ of ~~GtmA in complex with SAH~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5jgk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Blankenfeldt, W]]
	[[Category: Bock, T]]		[[Category: Bock, T]]
		+	[[Category: Dolan, S K]]
	[[Category: Dolye, S]]		[[Category: Dolye, S]]
-	[[Category: Dolan, S.K]]
-	[[Category: Blankenfeldt, W]]
	[[Category: Hering, V]]		[[Category: Hering, V]]
-	[[Category: Jones, G.W]]	+	[[Category: Jones, G W]]
		+	[[Category: Aspergillus fumigatus]]
		+	[[Category: Gliotoxin]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Resistance]]
		+	[[Category: S-adenosyl-l-homocysteine]]
		+	[[Category: Transferase]]

OCA at 23:19, 12 July 2016

OCA — Tue, 12 Jul 2016 23:19:41 GMT

←Older revision		Revision as of 23:19, 12 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5jgk is ON HOLD	+	The entry 5jgk is ON HOLD until Paper Publication

	Authors: Dolan, S.K., Bock, T., Hering, V., Jones, G.W., Blankenfeldt, W., Dolye, S.		Authors: Dolan, S.K., Bock, T., Hering, V., Jones, G.W., Blankenfeldt, W., Dolye, S.

OCA: Protected "5jgk" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 02:01:38 GMT

Protected "5jgk" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:01, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5jgk is ON HOLD Authors: Dolan, S.K., Bock, T., Hering, V., Jones, G.W., Blankenfeldt, W., Dolye, S. Description: Crystal structure of GtmA in comp...

OCA — Wed, 11 May 2016 02:01:37 GMT

New page: '''Unreleased structure''' The entry 5jgk is ON HOLD Authors: Dolan, S.K., Bock, T., Hering, V., Jones, G.W., Blankenfeldt, W., Dolye, S. Description: Crystal structure of GtmA in comp...

New page

'''Unreleased structure'''

The entry 5jgk is ON HOLD

Authors: Dolan, S.K., Bock, T., Hering, V., Jones, G.W., Blankenfeldt, W., Dolye, S.

Description: Crystal structure of GtmA in complex with SAH
[[Category: Unreleased Structures]]
[[Category: Bock, T]]
[[Category: Dolye, S]]
[[Category: Dolan, S.K]]
[[Category: Blankenfeldt, W]]
[[Category: Hering, V]]
[[Category: Jones, G.W]]