5jhy - Revision history

OCA at 18:52, 20 September 2023

OCA — Wed, 20 Sep 2023 18:52:44 GMT

←Older revision		Revision as of 18:52, 20 September 2023
Line 1:		Line 1:

	==Crystal Structure of Fungal MagKatG2 at pH 5.5==		==Crystal Structure of Fungal MagKatG2 at pH 5.5==
-	<StructureSection load='5jhy' size='340' side='right' caption='[[5jhy]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='5jhy' size='340' side='right'caption='[[5jhy]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jhy]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mago7 Mago7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JHY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jhy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyricularia_oryzae_70-15 Pyricularia oryzae 70-15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JHY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">KATG2, CPXB, MagKatG2, MGG_09834 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=242507 MAGO7])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Catalase_peroxidase Catalase peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.21 1.11.1.21]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jhy OCA], [https://pdbe.org/5jhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jhy RCSB], [https://www.ebi.ac.uk/pdbsum/5jhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jhy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jhy OCA], [~~http~~://pdbe.org/5jhy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jhy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jhy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jhy ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KATG2_MAGO7 KATG2_MAGO7]] Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Confers resistance to H(2)O(2) in hyphae. May play an antioxidative role in fungal defense against the host-produced H(2)O(2) (oxidative burst) at the early stage of plant infection.<ref>PMID:21043575</ref> <ref>PMID:21971530</ref>	+	[https://www.uniprot.org/uniprot/KATG2_MAGO7 KATG2_MAGO7] Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Confers resistance to H(2)O(2) in hyphae. May play an antioxidative role in fungal defense against the host-produced H(2)O(2) (oxidative burst) at the early stage of plant infection.<ref>PMID:21043575</ref> <ref>PMID:21971530</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:

	==See Also==		==See Also==
-	*[[Catalase\|Catalase]]	+	*[[Catalase 3D structures\|Catalase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Catalase peroxidase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Mago7~~]]	+	[[Category: Pyricularia oryzae 70-15]]
-	[[Category: Carpena, X]]	+	[[Category: Carpena X]]
-	[[Category: Gasselhuber, B]]	+	[[Category: Gasselhuber B]]
-	[[Category: Obinger, C]]	+	[[Category: Obinger C]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:13, 21 February 2019

OCA — Thu, 21 Feb 2019 07:13:06 GMT

←Older revision		Revision as of 07:13, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='5jhy' size='340' side='right' caption='[[5jhy]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='5jhy' size='340' side='right' caption='[[5jhy]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jhy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mago7 Mago7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JHY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KATG2, CPXB, MagKatG2, MGG_09834 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=242507 MAGO7])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase_peroxidase Catalase peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.21 1.11.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase_peroxidase Catalase peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.21 1.11.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jhy OCA], [http://pdbe.org/5jhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jhy RCSB], [http://www.ebi.ac.uk/pdbsum/5jhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jhy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jhy OCA], [http://pdbe.org/5jhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jhy RCSB], [http://www.ebi.ac.uk/pdbsum/5jhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jhy ProSAT]</span></td></tr>
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5jhy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5jhy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Catalase\|Catalase]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Catalase peroxidase]]		[[Category: Catalase peroxidase]]
		+	[[Category: Mago7]]
	[[Category: Carpena, X]]		[[Category: Carpena, X]]
	[[Category: Gasselhuber, B]]		[[Category: Gasselhuber, B]]
	[[Category: Obinger, C]]		[[Category: Obinger, C]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 17:45, 12 July 2016

OCA — Tue, 12 Jul 2016 17:45:25 GMT

←Older revision		Revision as of 17:45, 12 July 2016
Line 6:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase_peroxidase Catalase peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.21 1.11.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase_peroxidase Catalase peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.21 1.11.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jhy OCA], [http://pdbe.org/5jhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jhy RCSB], [http://www.ebi.ac.uk/pdbsum/5jhy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jhy OCA], [http://pdbe.org/5jhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jhy RCSB], [http://www.ebi.ac.uk/pdbsum/5jhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jhy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 23:12, 23 June 2016

OCA — Thu, 23 Jun 2016 23:12:05 GMT

←Older revision		Revision as of 23:12, 23 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5jhy is ~~ON HOLD~~	+	==Crystal Structure of Fungal MagKatG2 at pH 5.5==
		+	<StructureSection load='5jhy' size='340' side='right' caption='[[5jhy]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5jhy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JHY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase_peroxidase Catalase peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.21 1.11.1.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jhy OCA], [http://pdbe.org/5jhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jhy RCSB], [http://www.ebi.ac.uk/pdbsum/5jhy PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KATG2_MAGO7 KATG2_MAGO7]] Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Confers resistance to H(2)O(2) in hyphae. May play an antioxidative role in fungal defense against the host-produced H(2)O(2) (oxidative burst) at the early stage of plant infection.<ref>PMID:21043575</ref> <ref>PMID:21971530</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Catalase-peroxidases (KatGs) are unique bifunctional heme peroxidases with an additional posttranslationally formed redox-active Met-Tyr-Trp cofactor that is essential for catalase activity. On the basis of studies of bacterial KatGs, controversial mechanisms of hydrogen peroxide oxidation were proposed. The recent discovery of eukaryotic KatGs with differing pH optima of catalase activity now allows us to scrutinize those postulated reaction mechanisms. In our study, secreted KatG from the fungus Magnaporthe grisea (MagKatG2) was used to analyze the role of a remote KatG-typical mobile arginine that was shown to interact with the Met-Tyr-Trp adduct in a pH-dependent manner in bacterial KatGs. Here we present crystal structures of MagKatG2 at pH 3.0, 5.5, and 7.0 and investigate the mobility of Arg461 by molecular dynamics simulation. Data suggest that at pH >/=4.5 Arg461 mostly interacts with the deprotonated adduct Tyr. Elimination of Arg461 by mutation to Ala slightly increases the thermal stability but does not alter the active site architecture or the kinetics of cyanide binding. However, the variant Arg461Ala lost the wild-type-typical optimum of catalase activity at pH 5.25 (kcat = 6450 s-1) but exhibits a broad plateau between pH 4.5 and 7.5 (kcat = 270 s-1 at pH 5.5). Moreover, significant differences in the kinetics of interconversion of redox intermediates of wild-type and mutant protein mixed with either peroxyacetic acid or hydrogen peroxide are observed. These findings together with published data from bacterial KatGs allow us to propose a role of Arg461 in the H2O2 oxidation reaction of KatG.

-	~~Authors: Gasselhuber~~, B., ~~Obinger~~, C., ~~Carpena~~, X.	+	Interaction with the Redox Cofactor MYW and Functional Role of a Mobile Arginine in Eukaryotic Catalase-Peroxidase.,Gasselhuber B, Graf MM, Jakopitsch C, Zamocky M, Nicolussi A, Furtmuller PG, Oostenbrink C, Carpena X, Obinger C Biochemistry. 2016 Jun 16. PMID:27293030<ref>PMID:27293030</ref>

-	~~Description: Crystal Structure~~ of ~~Fungal MagKatG2 at pH 5~~.5	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5jhy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Catalase peroxidase]]
	[[Category: Carpena, X]]		[[Category: Carpena, X]]
-	[[Category: Obinger, C]]
	[[Category: Gasselhuber, B]]		[[Category: Gasselhuber, B]]
		+	[[Category: Obinger, C]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "5jhy" [edit=sysop:move=sysop]

OCA — Wed, 11 May 2016 02:05:55 GMT

Protected "5jhy" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:05, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5jhy is ON HOLD Authors: Gasselhuber, B., Obinger, C., Carpena, X. Description: Crystal Structure of Fungal MagKatG2 at pH 5.5 [[Category: Unreleas...

OCA — Wed, 11 May 2016 02:05:53 GMT

New page: '''Unreleased structure''' The entry 5jhy is ON HOLD Authors: Gasselhuber, B., Obinger, C., Carpena, X. Description: Crystal Structure of Fungal MagKatG2 at pH 5.5 [[Category: Unreleas...

New page

'''Unreleased structure'''

The entry 5jhy is ON HOLD

Authors: Gasselhuber, B., Obinger, C., Carpena, X.

Description: Crystal Structure of Fungal MagKatG2 at pH 5.5
[[Category: Unreleased Structures]]
[[Category: Carpena, X]]
[[Category: Obinger, C]]
[[Category: Gasselhuber, B]]