5jqn - Revision history

OCA at 19:02, 20 September 2023

OCA — Wed, 20 Sep 2023 19:02:33 GMT

←Older revision		Revision as of 19:02, 20 September 2023
Line 3:		Line 3:
	<StructureSection load='5jqn' size='340' side='right'caption='[[5jqn]], [[Resolution\|resolution]] 1.19Å' scene=''>		<StructureSection load='5jqn' size='340' side='right'caption='[[5jqn]], [[Resolution\|resolution]] 1.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jqn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Nesterenkonia_sp.~~_an1~~ Nesterenkonia sp. ~~an1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JQN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5JQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jqn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nesterenkonia_sp._AN1 Nesterenkonia sp. AN1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JQN FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.19Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">nit2 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=652017 Nesterenkonia sp. AN1])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Amidase Amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.4 3.5.1.4]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jqn OCA], [https://pdbe.org/5jqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jqn RCSB], [https://www.ebi.ac.uk/pdbsum/5jqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jqn ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5jqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jqn OCA], [~~http~~://pdbe.org/5jqn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jqn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jqn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jqn ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/C6K3Z5_9MICC C6K3Z5_9MICC]
-	Nesterenkonia strain AN1 was isolated from a screening program for nitrile- and amide-hydrolyzing microorganisms in Antarctic desert soil samples. Strain AN1 showed significant 16S rRNA sequence identity to known members of the genus. Like known Nesterenkonia species, strain AN1 was obligately alkaliphilic (optimum environmental pH, 9 to 10) and halotolerant (optimum environmental Na(+) content, 0 to 15% [wt/vol]) but was also shown to be an obligate psychrophile with optimum growth at approximately 21 degrees C. The partially sequenced genome of AN1 revealed an open reading frame (ORF) encoding a putative protein member of the nitrilase superfamily, referred to as NitN (264 amino acids). The protein crystallized readily as a dimer and the atomic structure of all but 10 amino acids of the protein was determined, confirming that the enzyme had an active site and a fold characteristic of the nitrilase superfamily. The protein was screened for activity against a variety of nitrile, carbamoyl, and amide substrates and was found to have only amidase activity. It had highest affinity for propionamide but demonstrated a low catalytic rate. NitN had maximal activity at 30 degrees C and between pH 6.5 and 7.5, conditions which are outside the optimum growth range for the organism.	+
-		+
-	Unique aliphatic amidase from a psychrotrophic and haloalkaliphilic nesterenkonia isolate.,Nel AJ, Tuffin IM, Sewell BT, Cowan DA Appl Environ Microbiol. 2011 Jun;77(11):3696-702. Epub 2011 Apr 15. PMID:21498772<ref>PMID:~~21498772<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5jqn" style="background-color:#fffaf0;"><~~/~~div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Amidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nesterenkonia sp. ~~an1~~]]	+	[[Category: Nesterenkonia sp. AN1]]
-	[[Category: Kimani~~, S W~~]]	+	[[Category: Kimani SW]]
-	[[Category: Sewell~~, B T~~]]	+	[[Category: Sewell BT]]
-	[[Category: Weber~~, B W]]~~	+	[[Category: Weber BW]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Neterenkonia sp. an1]]~~	+
-	~~[[Category: Nitn amidase~~]]	+

OCA at 11:34, 17 June 2020

OCA — Wed, 17 Jun 2020 11:34:07 GMT

←Older revision		Revision as of 11:34, 17 June 2020
Line 1:		Line 1:

	==NitN Amidase from Neterenkonia sp. AN1 after thrombin His-tag removal.==		==NitN Amidase from Neterenkonia sp. AN1 after thrombin His-tag removal.==
-	<StructureSection load='5jqn' size='340' side='right' caption='[[5jqn]], [[Resolution\|resolution]] 1.19Å' scene=''>	+	<StructureSection load='5jqn' size='340' side='right'caption='[[5jqn]], [[Resolution\|resolution]] 1.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jqn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JQN OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jqn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nesterenkonia_sp._an1 Nesterenkonia sp. an1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JQN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JQN FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nit2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=652017 Nesterenkonia sp. AN1])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amidase Amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.4 3.5.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amidase Amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.4 3.5.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jqn OCA], [http://pdbe.org/5jqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jqn RCSB], [http://www.ebi.ac.uk/pdbsum/5jqn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jqn OCA], [http://pdbe.org/5jqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jqn RCSB], [http://www.ebi.ac.uk/pdbsum/5jqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jqn ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Amidase]]		[[Category: Amidase]]
		+	[[Category: Large Structures]]
		+	[[Category: Nesterenkonia sp. an1]]
	[[Category: Kimani, S W]]		[[Category: Kimani, S W]]
	[[Category: Sewell, B T]]		[[Category: Sewell, B T]]

OCA at 15:58, 1 June 2016

OCA — Wed, 01 Jun 2016 15:58:31 GMT

←Older revision		Revision as of 15:58, 1 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5jqn is ~~ON HOLD~~	+	==NitN Amidase from Neterenkonia sp. AN1 after thrombin His-tag removal.==
		+	<StructureSection load='5jqn' size='340' side='right' caption='[[5jqn]], [[Resolution\|resolution]] 1.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5jqn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JQN FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amidase Amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.4 3.5.1.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jqn OCA], [http://pdbe.org/5jqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jqn RCSB], [http://www.ebi.ac.uk/pdbsum/5jqn PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nesterenkonia strain AN1 was isolated from a screening program for nitrile- and amide-hydrolyzing microorganisms in Antarctic desert soil samples. Strain AN1 showed significant 16S rRNA sequence identity to known members of the genus. Like known Nesterenkonia species, strain AN1 was obligately alkaliphilic (optimum environmental pH, 9 to 10) and halotolerant (optimum environmental Na(+) content, 0 to 15% [wt/vol]) but was also shown to be an obligate psychrophile with optimum growth at approximately 21 degrees C. The partially sequenced genome of AN1 revealed an open reading frame (ORF) encoding a putative protein member of the nitrilase superfamily, referred to as NitN (264 amino acids). The protein crystallized readily as a dimer and the atomic structure of all but 10 amino acids of the protein was determined, confirming that the enzyme had an active site and a fold characteristic of the nitrilase superfamily. The protein was screened for activity against a variety of nitrile, carbamoyl, and amide substrates and was found to have only amidase activity. It had highest affinity for propionamide but demonstrated a low catalytic rate. NitN had maximal activity at 30 degrees C and between pH 6.5 and 7.5, conditions which are outside the optimum growth range for the organism.

-	~~Authors: Sewell, B.T~~., ~~Kimani~~, ~~S.W.~~, ~~Weber~~, B.W.	+	Unique aliphatic amidase from a psychrotrophic and haloalkaliphilic nesterenkonia isolate.,Nel AJ, Tuffin IM, Sewell BT, Cowan DA Appl Environ Microbiol. 2011 Jun;77(11):3696-702. Epub 2011 Apr 15. PMID:21498772<ref>PMID:21498772</ref>

-	~~Description: NitN Amidase from Neterenkonia sp~~. ~~AN1 after thrombin His-tag removal~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Weber~~, B.W]]	+	<div class="pdbe-citations 5jqn" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Kimani~~, ~~S.W~~]]	+	== References ==
-	[[Category: ~~Sewell~~, B.T]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Amidase]]
		+	[[Category: Kimani, S W]]
		+	[[Category: Sewell, B T]]
		+	[[Category: Weber, B W]]
		+	[[Category: Hydrolase]]
		+	[[Category: Neterenkonia sp. an1]]
		+	[[Category: Nitn amidase]]

OCA: Protected "5jqn" [edit=sysop:move=sysop]

OCA — Sun, 15 May 2016 15:46:20 GMT

Protected "5jqn" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:46, 15 May 2016

OCA: New page: '''Unreleased structure''' The entry 5jqn is ON HOLD Authors: Sewell, B.T., Kimani, S.W., Weber, B.W. Description: NitN Amidase from Neterenkonia sp. AN1 after thrombin His-tag removal...

OCA — Sun, 15 May 2016 15:46:19 GMT

New page: '''Unreleased structure''' The entry 5jqn is ON HOLD Authors: Sewell, B.T., Kimani, S.W., Weber, B.W. Description: NitN Amidase from Neterenkonia sp. AN1 after thrombin His-tag removal...

New page

'''Unreleased structure'''

The entry 5jqn is ON HOLD

Authors: Sewell, B.T., Kimani, S.W., Weber, B.W.

Description: NitN Amidase from Neterenkonia sp. AN1 after thrombin His-tag removal.
[[Category: Unreleased Structures]]
[[Category: Weber, B.W]]
[[Category: Kimani, S.W]]
[[Category: Sewell, B.T]]