5jtq - Revision history

OCA at 07:24, 1 May 2024

OCA — Wed, 01 May 2024 07:24:45 GMT

←Older revision		Revision as of 07:24, 1 May 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SECB_ECOLI SECB_ECOLI] One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE.[HAMAP-Rule:MF_00821]		[https://www.uniprot.org/uniprot/SECB_ECOLI SECB_ECOLI] One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE.[HAMAP-Rule:MF_00821]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Molecular chaperones act on non-native proteins in the cell to prevent their aggregation, premature folding or misfolding. Different chaperones often exert distinct effects, such as acceleration or delay of folding, on client proteins via mechanisms that are poorly understood. Here we report the solution structure of SecB, a chaperone that exhibits strong antifolding activity, in complex with alkaline phosphatase and maltose-binding protein captured in their unfolded states. SecB uses long hydrophobic grooves that run around its disk-like shape to recognize and bind to multiple hydrophobic segments across the length of non-native proteins. The multivalent binding mode results in proteins wrapping around SecB. This unique complex architecture alters the kinetics of protein binding to SecB and confers strong antifolding activity on the chaperone. The data show how the different architectures of chaperones result in distinct binding modes with non-native proteins that ultimately define the activity of the chaperone.
-
-	Structural basis for the antifolding activity of a molecular chaperone.,Huang C, Rossi P, Saio T, Kalodimos CG Nature. 2016 Aug 8. doi: 10.1038/nature18965. PMID:27501151<ref>PMID:27501151</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5jtq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Maltose-binding protein 3D structures\|Maltose-binding protein 3D structures]]		*[[Maltose-binding protein 3D structures\|Maltose-binding protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 19:06, 20 September 2023

OCA — Wed, 20 Sep 2023 19:06:16 GMT

←Older revision		Revision as of 19:06, 20 September 2023
Line 1:		Line 1:

	==The structure of chaperone SecB in complex with unstructured MBP binding site d==		==The structure of chaperone SecB in complex with unstructured MBP binding site d==
-	<StructureSection load='5jtq' size='340' side='right'caption='[[5jtq~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5jtq' size='340' side='right'caption='[[5jtq]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jtq]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JTQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5JTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jtq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JTQ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5jtl\|5jtl]], [[5jtm\|5jtm]], [[5jtn\|5jtn]], [[5jto\|5jto]], [[5jtp\|5jtp]], [[5jtr\|5jtr]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">secB, Z5036, ECs4487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57]), malE, Z5632, ECs5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jtq OCA], [https://pdbe.org/5jtq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jtq RCSB], [https://www.ebi.ac.uk/pdbsum/5jtq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jtq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5jtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jtq OCA], [~~http~~://pdbe.org/5jtq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jtq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jtq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jtq ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~SECB_ECO57 SECB_ECO57]~~] One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. [~~[http~~:~~//www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57~~]~~] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).~~	+	[https://www.uniprot.org/uniprot/SECB_ECOLI SECB_ECOLI] One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE.[HAMAP-Rule:MF_00821]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eco57~~]]	+	[[Category: Escherichia coli O157:H7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Huang, C]]	+	[[Category: Huang C]]
-	[[Category: Kalodimos~~, C G~~]]	+	[[Category: Kalodimos CG]]
-	[[Category: Rossi, P]]	+	[[Category: Rossi P]]
-	[[Category: Saio, T]]	+	[[Category: Saio T]]
-	~~[[Category: Chaperone-protein binding complex]]~~	+
-	~~[[Category: Molecular chaperone~~]]	+

OCA at 11:39, 17 June 2020

OCA — Wed, 17 Jun 2020 11:39:29 GMT

←Older revision		Revision as of 11:39, 17 June 2020
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==The structure of chaperone SecB in complex with unstructured MBP binding site d==		==The structure of chaperone SecB in complex with unstructured MBP binding site d==
-	<StructureSection load='5jtq' size='340' side='right' caption='[[5jtq]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5jtq' size='340' side='right'caption='[[5jtq]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jtq]] is a 6 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JTQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jtq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JTQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JTQ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jtl\|5jtl]], [[5jtm\|5jtm]], [[5jtn\|5jtn]], [[5jto\|5jto]], [[5jtp\|5jtp]], [[5jtr\|5jtr]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jtl\|5jtl]], [[5jtm\|5jtm]], [[5jtn\|5jtn]], [[5jto\|5jto]], [[5jtp\|5jtp]], [[5jtr\|5jtr]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jtq OCA], [http://pdbe.org/5jtq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jtq RCSB], [http://www.ebi.ac.uk/pdbsum/5jtq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jtq ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">secB, Z5036, ECs4487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57]), malE, Z5632, ECs5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jtq OCA], [http://pdbe.org/5jtq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jtq RCSB], [http://www.ebi.ac.uk/pdbsum/5jtq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jtq ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SECB_ECO57 SECB_ECO57]] One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. [[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).		[[http://www.uniprot.org/uniprot/SECB_ECO57 SECB_ECO57]] One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. [[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5jtq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5jtq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Maltose-binding protein 3D structures\|Maltose-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Eco57]]
		+	[[Category: Large Structures]]
	[[Category: Huang, C]]		[[Category: Huang, C]]
	[[Category: Kalodimos, C G]]		[[Category: Kalodimos, C G]]

OCA at 06:53, 10 September 2016

OCA — Sat, 10 Sep 2016 06:53:03 GMT

←Older revision		Revision as of 06:53, 10 September 2016
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	{{Large structure}}
		+	==The structure of chaperone SecB in complex with unstructured MBP binding site d==
		+	<StructureSection load='5jtq' size='340' side='right' caption='[[5jtq]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5jtq]] is a 6 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JTQ FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jtl\|5jtl]], [[5jtm\|5jtm]], [[5jtn\|5jtn]], [[5jto\|5jto]], [[5jtp\|5jtp]], [[5jtr\|5jtr]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jtq OCA], [http://pdbe.org/5jtq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jtq RCSB], [http://www.ebi.ac.uk/pdbsum/5jtq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jtq ProSAT]</span></td></tr>
		+	</table>
		+	{{Large structure}}
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SECB_ECO57 SECB_ECO57]] One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. [[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Molecular chaperones act on non-native proteins in the cell to prevent their aggregation, premature folding or misfolding. Different chaperones often exert distinct effects, such as acceleration or delay of folding, on client proteins via mechanisms that are poorly understood. Here we report the solution structure of SecB, a chaperone that exhibits strong antifolding activity, in complex with alkaline phosphatase and maltose-binding protein captured in their unfolded states. SecB uses long hydrophobic grooves that run around its disk-like shape to recognize and bind to multiple hydrophobic segments across the length of non-native proteins. The multivalent binding mode results in proteins wrapping around SecB. This unique complex architecture alters the kinetics of protein binding to SecB and confers strong antifolding activity on the chaperone. The data show how the different architectures of chaperones result in distinct binding modes with non-native proteins that ultimately define the activity of the chaperone.

-	~~The entry 5jtq is ON HOLD until Paper Publication~~	+	Structural basis for the antifolding activity of a molecular chaperone.,Huang C, Rossi P, Saio T, Kalodimos CG Nature. 2016 Aug 8. doi: 10.1038/nature18965. PMID:27501151<ref>PMID:27501151</ref>

-	~~Authors: Huang~~, C.~~, Saio, T~~.~~, Rossi, P., Kalodimos, C.G~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~The structure of chaperone SecB in complex with unstructured MBP binding site d~~	+	<div class="pdbe-citations 5jtq" style="background-color:#fffaf0;"></div>
-	~~[[Category: Unreleased Structures]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Huang, C]]		[[Category: Huang, C]]
		+	[[Category: Kalodimos, C G]]
	[[Category: Rossi, P]]		[[Category: Rossi, P]]
	[[Category: Saio, T]]		[[Category: Saio, T]]
-	[[Category: ~~Kalodimos, C.G~~]]	+	[[Category: Chaperone-protein binding complex]]
		+	[[Category: Molecular chaperone]]

OCA at 13:06, 20 June 2016

OCA — Mon, 20 Jun 2016 13:06:38 GMT

←Older revision		Revision as of 13:06, 20 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5jtq is ON HOLD	+	The entry 5jtq is ON HOLD until Paper Publication

	Authors: Huang, C., Saio, T., Rossi, P., Kalodimos, C.G.		Authors: Huang, C., Saio, T., Rossi, P., Kalodimos, C.G.

OCA: Protected "5jtq" [edit=sysop:move=sysop]

OCA — Wed, 01 Jun 2016 13:49:24 GMT

Protected "5jtq" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:49, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5jtq is ON HOLD Authors: Huang, C., Saio, T., Rossi, P., Kalodimos, C.G. Description: The structure of chaperone SecB in complex with unstructured ...

OCA — Wed, 01 Jun 2016 13:49:23 GMT

New page: '''Unreleased structure''' The entry 5jtq is ON HOLD Authors: Huang, C., Saio, T., Rossi, P., Kalodimos, C.G. Description: The structure of chaperone SecB in complex with unstructured ...

New page

'''Unreleased structure'''

The entry 5jtq is ON HOLD

Authors: Huang, C., Saio, T., Rossi, P., Kalodimos, C.G.

Description: The structure of chaperone SecB in complex with unstructured MBP binding site d
[[Category: Unreleased Structures]]
[[Category: Huang, C]]
[[Category: Rossi, P]]
[[Category: Saio, T]]
[[Category: Kalodimos, C.G]]