5jy4 - Revision history

OCA at 19:14, 20 September 2023

OCA — Wed, 20 Sep 2023 19:14:44 GMT

←Older revision		Revision as of 19:14, 20 September 2023
Line 3:		Line 3:
	<StructureSection load='5jy4' size='340' side='right'caption='[[5jy4]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='5jy4' size='340' side='right'caption='[[5jy4]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jy4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JY4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JY4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jy4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JY4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=ISC:(5S~~,~~6S)-5-~~[~~(1-CARBOXYETHENYL)OXY~~]~~-6-HYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC+ACID'>ISC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.11Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=ISC:(5S,6S)-5-[(1-CARBOXYETHENYL)OXY]-6-HYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC+ACID'>ISC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat">[[5jy9\|5jy9]]~~, [~~[5jy8\|5jy8]~~], ~~[[5jxz\|5jxz]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jy4 OCA], [https://pdbe.org/5jy4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jy4 RCSB], [https://www.ebi.ac.uk/pdbsum/5jy4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jy4 ProSAT]</span></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">entC~~, ~~Z0735, ECs0632 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jy4 OCA], [~~http~~://pdbe.org/5jy4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jy4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jy4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jy4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ENTC_ECO57 ENTC_ECO57]] Involved in the biosynthesis of the siderophore enterobactin (macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine). Catalyzes the reversible conversion of chorismate to isochorismate.[UniProtKB:P0AEJ2]	+	[https://www.uniprot.org/uniprot/ENTC_ECO57 ENTC_ECO57] Involved in the biosynthesis of the siderophore enterobactin (macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine). Catalyzes the reversible conversion of chorismate to isochorismate.[UniProtKB:P0AEJ2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eco57]]~~	+	[[Category: Escherichia coli O157:H7]]
-	~~[[Category~~: ~~Isochorismate synthase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gulick~~, A M~~]]	+	[[Category: Gulick AM]]
-	[[Category: Lamb~~, A L~~]]	+	[[Category: Lamb AL]]
-	[[Category: Meneely~~, K M~~]]	+	[[Category: Meneely KM]]
-	[[Category: Sundlov~~, J A]]~~	+	[[Category: Sundlov JA]]
-	~~[[Category: Isochorismate]]~~	+
-	~~[[Category: Isomerase~~]]	+

OCA at 16:25, 11 December 2019

OCA — Wed, 11 Dec 2019 16:25:03 GMT

←Older revision		Revision as of 16:25, 11 December 2019
Line 1:		Line 1:

	==A high magnesium structure of the isochorismate synthase, EntC==		==A high magnesium structure of the isochorismate synthase, EntC==
-	<StructureSection load='5jy4' size='340' side='right' caption='[[5jy4]], [[Resolution\|resolution]] 2.11Å' scene=''>	+	<StructureSection load='5jy4' size='340' side='right'caption='[[5jy4]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5jy4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JY4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JY4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5jy4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JY4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JY4 FirstGlance]. <br>
Line 28:		Line 28:
	[[Category: Eco57]]		[[Category: Eco57]]
	[[Category: Isochorismate synthase]]		[[Category: Isochorismate synthase]]
		+	[[Category: Large Structures]]
	[[Category: Gulick, A M]]		[[Category: Gulick, A M]]
	[[Category: Lamb, A L]]		[[Category: Lamb, A L]]

OCA at 12:57, 6 November 2017

OCA — Mon, 06 Nov 2017 12:57:13 GMT

←Older revision		Revision as of 12:57, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5jy4' size='340' side='right' caption='[[5jy4]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='5jy4' size='340' side='right' caption='[[5jy4]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jy4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JY4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JY4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jy4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JY4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JY4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ISC:(5S,6S)-5-[(1-CARBOXYETHENYL)OXY]-6-HYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC+ACID'>ISC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ISC:(5S,6S)-5-[(1-CARBOXYETHENYL)OXY]-6-HYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC+ACID'>ISC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jy9\|5jy9]], [[5jy8\|5jy8]], [[5jxz\|5jxz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jy9\|5jy9]], [[5jy8\|5jy8]], [[5jxz\|5jxz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">entC, Z0735, ECs0632 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jy4 OCA], [http://pdbe.org/5jy4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jy4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jy4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jy4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jy4 OCA], [http://pdbe.org/5jy4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jy4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jy4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jy4 ProSAT]</span></td></tr>
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Eco57]]
	[[Category: Isochorismate synthase]]		[[Category: Isochorismate synthase]]
	[[Category: Gulick, A M]]		[[Category: Gulick, A M]]

OCA at 15:08, 26 July 2016

OCA — Tue, 26 Jul 2016 15:08:39 GMT

←Older revision		Revision as of 15:08, 26 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5jy4 is ~~ON HOLD until Paper~~ Publication	+	==A high magnesium structure of the isochorismate synthase, EntC==
		+	<StructureSection load='5jy4' size='340' side='right' caption='[[5jy4]], [[Resolution\|resolution]] 2.11Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5jy4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JY4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JY4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ISC:(5S,6S)-5-[(1-CARBOXYETHENYL)OXY]-6-HYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC+ACID'>ISC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jy9\|5jy9]], [[5jy8\|5jy8]], [[5jxz\|5jxz]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isochorismate_synthase Isochorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.4.2 5.4.4.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jy4 OCA], [http://pdbe.org/5jy4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jy4 RCSB], [http://www.ebi.ac.uk/pdbsum/5jy4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jy4 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENTC_ECO57 ENTC_ECO57]] Involved in the biosynthesis of the siderophore enterobactin (macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine). Catalyzes the reversible conversion of chorismate to isochorismate.[UniProtKB:P0AEJ2]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The shikimate pathway of bacteria, fungi, and plants generates chorismate, which is drawn into biosynthetic pathways that form aromatic amino acids and other important metabolites, including folates, menaquinone, and siderophores. Many of the pathways initiated at this branch point transform chorismate using an MST enzyme. The MST enzymes (menaquinone, siderophore, and tryptophan biosynthetic enzymes) are structurally homologous and magnesium-dependent, and all perform similar chemical permutations to chorismate by nucleophilic addition (hydroxyl or amine) at the 2-position of the ring, inducing displacement of the 4-hydroxyl. The isomerase enzymes release isochorismate or aminodeoxychorismate as the product, while the synthase enzymes also have lyase activity that displaces pyruvate to form either salicylate or anthranilate. This has led to the hypothesis that the isomerase and lyase activities performed by the MST enzymes are functionally conserved. Here we have developed tailored pre-steady-state approaches to establish the kinetic mechanisms of the isochorismate and salicylate synthase enzymes of siderophore biosynthesis. Our data are centered on the role of magnesium ions, which inhibit the isochorismate synthase enzymes but not the salicylate synthase enzymes. Prior structural data have suggested that binding of the metal ion occludes access or egress of substrates. Our kinetic data indicate that for the production of isochorismate, a high magnesium ion concentration suppresses the rate of release of product, accounting for the observed inhibition and establishing the basis of the ordered-addition kinetic mechanism. Moreover, we show that isochorismate is channeled through the synthase reaction as an intermediate that is retained in the active site by the magnesium ion. Indeed, the lyase-active enzyme has 3 orders of magnitude higher affinity for the isochorismate complex relative to the chorismate complex. Apparent negative-feedback inhibition by ferrous ions is documented at nanomolar concentrations, which is a potentially physiologically relevant mode of regulation for siderophore biosynthesis in vivo.

-	~~Authors~~: Meneely~~, K.M.~~, Sundlov~~, J.A.~~, Gulick, ~~A.M.~~, Lamb~~, A~~.L.	+	An Open and Shut Case: The Interaction of Magnesium with MST Enzymes.,Meneely KM, Sundlov JA, Gulick AM, Moran GR, Lamb AL J Am Chem Soc. 2016 Jul 19. PMID:27373320<ref>PMID:27373320</ref>

-	~~Description: A high magnesium structure~~ of the ~~isochorismate synthase, EntC~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Sundlov~~, J.A]]	+	<div class="pdbe-citations 5jy4" style="background-color:#fffaf0;"></div>
-	[[Category: Lamb, A.L]]	+	== References ==
-	[[Category: Meneely, K.M]]	+	<references/>
-	[[Category: ~~Gulick~~, A.M]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Isochorismate synthase]]
		+	[[Category: Gulick, A M]]
		+	[[Category: Lamb, A L]]
		+	[[Category: Meneely, K M]]
		+	[[Category: Sundlov, J A]]
		+	[[Category: Isochorismate]]
		+	[[Category: Isomerase]]

OCA at 13:14, 20 June 2016

OCA — Mon, 20 Jun 2016 13:14:47 GMT

←Older revision		Revision as of 13:14, 20 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5jy4 is ON HOLD	+	The entry 5jy4 is ON HOLD until Paper Publication

	Authors: Meneely, K.M., Sundlov, J.A., Gulick, A.M., Lamb, A.L.		Authors: Meneely, K.M., Sundlov, J.A., Gulick, A.M., Lamb, A.L.

OCA: Protected "5jy4" [edit=sysop:move=sysop]

OCA — Wed, 01 Jun 2016 14:02:13 GMT

Protected "5jy4" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:02, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5jy4 is ON HOLD Authors: Meneely, K.M., Sundlov, J.A., Gulick, A.M., Lamb, A.L. Description: A high magnesium structure of the isochorismate syntha...

OCA — Wed, 01 Jun 2016 14:02:12 GMT

New page: '''Unreleased structure''' The entry 5jy4 is ON HOLD Authors: Meneely, K.M., Sundlov, J.A., Gulick, A.M., Lamb, A.L. Description: A high magnesium structure of the isochorismate syntha...

New page

'''Unreleased structure'''

The entry 5jy4 is ON HOLD

Authors: Meneely, K.M., Sundlov, J.A., Gulick, A.M., Lamb, A.L.

Description: A high magnesium structure of the isochorismate synthase, EntC
[[Category: Unreleased Structures]]
[[Category: Sundlov, J.A]]
[[Category: Lamb, A.L]]
[[Category: Meneely, K.M]]
[[Category: Gulick, A.M]]