5k24 - Revision history

OCA at 19:18, 20 September 2023

OCA — Wed, 20 Sep 2023 19:18:41 GMT

←Older revision		Revision as of 19:18, 20 September 2023
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	==Crystal structure of the complex between phosphatase PRL-2 in the oxidized state with the Bateman domain of murine magnesium transporter CNNM3==		==Crystal structure of the complex between phosphatase PRL-2 in the oxidized state with the Bateman domain of murine magnesium transporter CNNM3==
-	<StructureSection load='5k24' size='340' side='right' caption='[[5k24]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='5k24' size='340' side='right'caption='[[5k24]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k24]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K24 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5K24 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k24]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K24 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k22\|5k22]]~~, [[~~5k23~~\|~~5k23]], [[5k25\|5k25~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Ptp4a2, Prl2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10090 LK3 transgenic mice]), Cnnm3, Acdp3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k24 OCA], [https://pdbe.org/5k24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k24 RCSB], [https://www.ebi.ac.uk/pdbsum/5k24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k24 ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5k24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k24 OCA], [~~http~~://pdbe.org/5k24 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5k24 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5k24 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5k24 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TP4A2_MOUSE TP4A2_MOUSE]] Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB (By similarity)~~. [[http://www.uniprot.org/uniprot/CNNM3_MOUSE CNNM3_MOUSE]] Probable metal transporter~~.	+	[https://www.uniprot.org/uniprot/TP4A2_MOUSE TP4A2_MOUSE] Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 21:

	==See Also==		==See Also==
-	*[[Tyrosine phosphatase\|Tyrosine phosphatase]]	+	*[[Dual specificity phosphatase 3D structures\|Dual specificity phosphatase 3D structures]]
		+	*[[Tyrosine phosphatase 3D structures\|Tyrosine phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Protein-tyrosine-phosphatase~~]]	+	[[Category: Mus musculus]]
-	[[Category: Gehring, K]]	+	[[Category: Gehring K]]
-	[[Category: Gulerez, I]]	+	[[Category: Gulerez I]]
-	[[Category: Kozlov, G]]	+	[[Category: Kozlov G]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-transporter protein-protein binding complex]]~~	+
-	~~[[Category: Magnesium transporter]]~~	+
-	~~[[Category: Phosphatase]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 08:24, 3 October 2018

OCA — Wed, 03 Oct 2018 08:24:19 GMT

←Older revision		Revision as of 08:24, 3 October 2018
Line 3:		Line 3:
	<StructureSection load='5k24' size='340' side='right' caption='[[5k24]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='5k24' size='340' side='right' caption='[[5k24]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k24]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K24 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K24 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k24]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K24 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K24 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k22\|5k22]], [[5k23\|5k23]], [[5k25\|5k25]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k22\|5k22]], [[5k23\|5k23]], [[5k25\|5k25]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ptp4a2, Prl2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Cnnm3, Acdp3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k24 OCA], [http://pdbe.org/5k24 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k24 RCSB], [http://www.ebi.ac.uk/pdbsum/5k24 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k24 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k24 OCA], [http://pdbe.org/5k24 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k24 RCSB], [http://www.ebi.ac.uk/pdbsum/5k24 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k24 ProSAT]</span></td></tr>
Line 11:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/TP4A2_MOUSE TP4A2_MOUSE]] Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB (By similarity). [[http://www.uniprot.org/uniprot/CNNM3_MOUSE CNNM3_MOUSE]] Probable metal transporter.		[[http://www.uniprot.org/uniprot/TP4A2_MOUSE TP4A2_MOUSE]] Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB (By similarity). [[http://www.uniprot.org/uniprot/CNNM3_MOUSE CNNM3_MOUSE]] Probable metal transporter.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The phosphatases of regenerating liver (PRLs) are involved in tumorigenesis and metastatic cancer yet their cellular function remains unclear. Recent reports have shown PRL phosphatases bind tightly to the CNNM family of membrane proteins to regulate magnesium efflux. Here, we characterize the interactions between the CBS-pair (Bateman) domain of CNNM3 and either PRL2 or PRL3 using X-ray crystallography, isothermal titration calorimetry, and activity assays. We report four new crystal structures of PRL proteins bound to the CNNM3 CBS-pair domain that reveal the effects of cysteine disulphide formation and nucleotide binding on complex formation. We use comprehensive mutagenesis of the PRL3 catalytic site to quantify the importance of different PRL amino acids, including cysteine 104, leucine 108, and arginine 110, for CNNM binding and phosphatase activity. We show the PRL3 R138E mutant is selectively deficient in CNNM3 binding with the potential to distinguish between the downstream effects of phosphatase and CNNM-binding activities in vivo. Through a novel activity assay, we show that PRL3 has magnesium-sensitive phosphatase activity with ATP and other nucleotides. Our results identify a strong correlation between phosphatase activity and CNNM binding and support the contention that PRL function as pseudophosphatases regulated by chemical modifications of their catalytic cysteine.
		+
		+	PRL3 phosphatase active site is required for binding the putative magnesium transporter CNNM3.,Zhang H, Kozlov G, Li X, Wu H, Gulerez I, Gehring K Sci Rep. 2017 Mar 3;7(1):48. doi: 10.1038/s41598-017-00147-2. PMID:28246390<ref>PMID:28246390</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5k24" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tyrosine phosphatase\|Tyrosine phosphatase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Protein-tyrosine-phosphatase]]		[[Category: Protein-tyrosine-phosphatase]]
	[[Category: Gehring, K]]		[[Category: Gehring, K]]

OCA at 17:43, 19 October 2016

OCA — Wed, 19 Oct 2016 17:43:01 GMT

←Older revision		Revision as of 17:43, 19 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5k24 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the complex between phosphatase PRL-2 in the oxidized state with the Bateman domain of murine magnesium transporter CNNM3==
-		+	<StructureSection load='5k24' size='340' side='right' caption='[[5k24]], [[Resolution\|resolution]] 3.10Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5k24]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K24 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K24 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k22\|5k22]], [[5k23\|5k23]], [[5k25\|5k25]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k24 OCA], [http://pdbe.org/5k24 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k24 RCSB], [http://www.ebi.ac.uk/pdbsum/5k24 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k24 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TP4A2_MOUSE TP4A2_MOUSE]] Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB (By similarity). [[http://www.uniprot.org/uniprot/CNNM3_MOUSE CNNM3_MOUSE]] Probable metal transporter.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Protein-tyrosine-phosphatase]]
		+	[[Category: Gehring, K]]
		+	[[Category: Gulerez, I]]
		+	[[Category: Kozlov, G]]
		+	[[Category: Complex]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-transporter protein-protein binding complex]]
		+	[[Category: Magnesium transporter]]
		+	[[Category: Phosphatase]]
		+	[[Category: Protein binding]]

OCA at 13:21, 20 June 2016

OCA — Mon, 20 Jun 2016 13:21:32 GMT

←Older revision		Revision as of 13:21, 20 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5k24 is ON HOLD	+	The entry 5k24 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5k24" [edit=sysop:move=sysop]

OCA — Wed, 01 Jun 2016 14:12:43 GMT

Protected "5k24" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:12, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5k24 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Jun 2016 14:12:42 GMT

New page: '''Unreleased structure''' The entry 5k24 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5k24 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]