5k8n - Revision history

OCA at 10:43, 27 September 2023

OCA — Wed, 27 Sep 2023 10:43:15 GMT

←Older revision		Revision as of 10:43, 27 September 2023
Line 3:		Line 3:
	<StructureSection load='5k8n' size='340' side='right'caption='[[5k8n]], [[Resolution\|resolution]] 3.23Å' scene=''>		<StructureSection load='5k8n' size='340' side='right'caption='[[5k8n]], [[Resolution\|resolution]] 3.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k8n]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Brasz Brasz~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K8N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5K8N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k8n]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_sp. Bradyrhizobium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K8N FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=6R6:5~~-~~NITROANTHRANILIC+ACID'>6R6</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.225Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k8m\|5k8m]]~~, [[~~5k8o~~\|~~5k8o~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6R6:5-NITROANTHRANILIC+ACID'>6R6</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>naaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=376 BRASZ])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k8n OCA], [https://pdbe.org/5k8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k8n RCSB], [https://www.ebi.ac.uk/pdbsum/5k8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k8n ProSAT]</span></td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/~~5-~~nitroanthranilic_acid_aminohydrolase 5-nitroanthranilic acid aminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.8 3.5.99.8]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5k8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k8n OCA], [~~http~~://pdbe.org/5k8n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5k8n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5k8n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5k8n ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NAAA_BRASZ NAAA_BRASZ]] Catalyzes the deamination of 5-nitroanthranilate (5NAA) to 5-nitrosalicylate (5NSA), the first step in biodegradation of 5-nitroanthranilate.<ref>PMID:20081004</ref> <ref>PMID:21498645</ref>	+	[https://www.uniprot.org/uniprot/NAAA_BRASZ NAAA_BRASZ] Catalyzes the deamination of 5-nitroanthranilate (5NAA) to 5-nitrosalicylate (5NSA), the first step in biodegradation of 5-nitroanthranilate.<ref>PMID:20081004</ref> <ref>PMID:21498645</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~5-nitroanthranilic acid aminohydrolase]]~~	+	[[Category: Bradyrhizobium sp]]
-	~~[[Category: Brasz~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kalyoncu, S]]	+	[[Category: Kalyoncu S]]
-	~~[[Category: Deaminase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metalloenzyme]]~~	+
-	~~[[Category: Nitroaromatic~~]]	+

OCA at 09:27, 1 July 2020

OCA — Wed, 01 Jul 2020 09:27:11 GMT

←Older revision		Revision as of 09:27, 1 July 2020
Line 1:		Line 1:

	==5NAA-bound 5-nitroanthranilate aminohydrolase==		==5NAA-bound 5-nitroanthranilate aminohydrolase==
-	<StructureSection load='5k8n' size='340' side='right' caption='[[5k8n]], [[Resolution\|resolution]] 3.23Å' scene=''>	+	<StructureSection load='5k8n' size='340' side='right'caption='[[5k8n]], [[Resolution\|resolution]] 3.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k8n]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K8N OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5K8N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k8n]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Brasz Brasz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K8N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5K8N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6R6:5-NITROANTHRANILIC+ACID'>6R6</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6R6:5-NITROANTHRANILIC+ACID'>6R6</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k8m\|5k8m]], [[5k8o\|5k8o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k8m\|5k8m]], [[5k8o\|5k8o]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">naaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=376 BRASZ])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-nitroanthranilic_acid_aminohydrolase 5-nitroanthranilic acid aminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.8 3.5.99.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-nitroanthranilic_acid_aminohydrolase 5-nitroanthranilic acid aminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.8 3.5.99.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5k8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k8n OCA], [http://pdbe.org/5k8n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k8n RCSB], [http://www.ebi.ac.uk/pdbsum/5k8n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k8n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5k8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k8n OCA], [http://pdbe.org/5k8n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k8n RCSB], [http://www.ebi.ac.uk/pdbsum/5k8n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k8n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: 5-nitroanthranilic acid aminohydrolase]]		[[Category: 5-nitroanthranilic acid aminohydrolase]]
		+	[[Category: Brasz]]
		+	[[Category: Large Structures]]
	[[Category: Kalyoncu, S]]		[[Category: Kalyoncu, S]]
	[[Category: Deaminase]]		[[Category: Deaminase]]

OCA at 09:43, 19 October 2016

OCA — Wed, 19 Oct 2016 09:43:27 GMT

←Older revision		Revision as of 09:43, 19 October 2016
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/NAAA_BRASZ NAAA_BRASZ]] Catalyzes the deamination of 5-nitroanthranilate (5NAA) to 5-nitrosalicylate (5NSA), the first step in biodegradation of 5-nitroanthranilate.<ref>PMID:20081004</ref> <ref>PMID:21498645</ref>		[[http://www.uniprot.org/uniprot/NAAA_BRASZ NAAA_BRASZ]] Catalyzes the deamination of 5-nitroanthranilate (5NAA) to 5-nitrosalicylate (5NSA), the first step in biodegradation of 5-nitroanthranilate.<ref>PMID:20081004</ref> <ref>PMID:21498645</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nitroaromatic compounds are typically toxic and resistant to degradation. Bradyrhizobium species strain JS329 metabolizes 5-nitroanthranilic acid (5NAA), which is a molecule secreted by Streptomyces scabies, the plant pathogen responsible for potato scab. The first biodegradation enzyme is 5NAA-aminohydrolase (5NAA-A), a metalloprotease family member that converts 5NAA to 5-nitrosalicylic acid. We characterized 5NAA-A biochemically and obtained snapshots of its mechanism. 5NAA-A, an octamer that can use several divalent transition metals for catalysis in vitro, employs a nucleophilic aromatic substitution mechanism. Unexpectedly, the metal in 5NAA-A is labile but is readily loaded in the presence of substrate. 5NAA-A is specific for 5NAA and cannot hydrolyze other tested derivatives, which are likewise poor inhibitors. The 5NAA-A structure and mechanism expand our understanding of the chemical ecology of an agriculturally important plant and pathogen, and will inform bioremediation and biocatalytic approaches to mitigate the environmental and ecological impact of nitroanilines and other challenging substrates.
		+
		+	Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution.,Kalyoncu S, Heaner DP Jr, Kurt Z, Bethel CM, Ukachukwu CU, Chakravarthy S, Spain JC, Lieberman RL Nat Chem Biol. 2016 Oct 3. doi: 10.1038/nchembio.2191. PMID:27694799<ref>PMID:27694799</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5k8n" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:32, 5 October 2016

OCA — Wed, 05 Oct 2016 21:32:25 GMT

←Older revision		Revision as of 21:32, 5 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5k8n is ~~ON HOLD until Oct 05 2017~~	+	==5NAA-bound 5-nitroanthranilate aminohydrolase==
-		+	<StructureSection load='5k8n' size='340' side='right' caption='[[5k8n]], [[Resolution\|resolution]] 3.23Å' scene=''>
-	~~Authors~~: ~~Kalyoncu~~, S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5k8n]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K8N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K8N FirstGlance]. <br>
-	~~Description~~: 5NAA-~~bound~~ 5-nitroanthranilate ~~aminohydrolase~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6R6:5-NITROANTHRANILIC+ACID'>6R6</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k8m\|5k8m]], [[5k8o\|5k8o]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-nitroanthranilic_acid_aminohydrolase 5-nitroanthranilic acid aminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.8 3.5.99.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k8n OCA], [http://pdbe.org/5k8n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k8n RCSB], [http://www.ebi.ac.uk/pdbsum/5k8n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k8n ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NAAA_BRASZ NAAA_BRASZ]] Catalyzes the deamination of 5-nitroanthranilate (5NAA) to 5-nitrosalicylate (5NSA), the first step in biodegradation of 5-nitroanthranilate.<ref>PMID:20081004</ref> <ref>PMID:21498645</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 5-nitroanthranilic acid aminohydrolase]]
	[[Category: Kalyoncu, S]]		[[Category: Kalyoncu, S]]
		+	[[Category: Deaminase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Metalloenzyme]]
		+	[[Category: Nitroaromatic]]

OCA at 10:28, 21 September 2016

OCA — Wed, 21 Sep 2016 10:28:30 GMT

←Older revision		Revision as of 10:28, 21 September 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5k8n is ON HOLD until ~~May 30 2018~~	+	The entry 5k8n is ON HOLD until Oct 05 2017

	Authors: Kalyoncu, S.		Authors: Kalyoncu, S.

OCA: Protected "5k8n" [edit=sysop:move=sysop]

OCA — Mon, 20 Jun 2016 13:33:55 GMT

Protected "5k8n" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:33, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5k8n is ON HOLD until May 30 2018 Authors: Kalyoncu, S. Description: 5NAA-bound 5-nitroanthranilate aminohydrolase [[Category: Unreleased Structure...

OCA — Mon, 20 Jun 2016 13:33:54 GMT

New page: '''Unreleased structure''' The entry 5k8n is ON HOLD until May 30 2018 Authors: Kalyoncu, S. Description: 5NAA-bound 5-nitroanthranilate aminohydrolase [[Category: Unreleased Structure...

New page

'''Unreleased structure'''

The entry 5k8n is ON HOLD until May 30 2018

Authors: Kalyoncu, S.

Description: 5NAA-bound 5-nitroanthranilate aminohydrolase
[[Category: Unreleased Structures]]
[[Category: Kalyoncu, S]]