5k90 - Revision history

OCA at 10:43, 27 September 2023

OCA — Wed, 27 Sep 2023 10:43:30 GMT

←Older revision		Revision as of 10:43, 27 September 2023
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	<StructureSection load='5k90' size='340' side='right'caption='[[5k90]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='5k90' size='340' side='right'caption='[[5k90]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k90]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cyap4 Cyap4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K90 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5K90 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k90]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyanothece_sp._PCC_7425 Cyanothece sp. PCC 7425]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K90 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IS8:isothiocyanate'>IS8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.28Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cyan7425_1434 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395961 CYAP4])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IS8:isothiocyanate'>IS8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5k90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k90 OCA], [~~http~~://pdbe.org/5k90 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5k90 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5k90 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5k90 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k90 OCA], [https://pdbe.org/5k90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k90 RCSB], [https://www.ebi.ac.uk/pdbsum/5k90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k90 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B8HNS6_CYAP4 B8HNS6_CYAP4]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cyap4~~]]	+	[[Category: Cyanothece sp. PCC 7425]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Djinovic-Carugo, K]]	+	[[Category: Djinovic-Carugo K]]
-	[[Category: Mlynek, G]]	+	[[Category: Mlynek G]]
-	[[Category: Obinger, C]]	+	[[Category: Obinger C]]
-	[[Category: Puehringer, D]]	+	[[Category: Puehringer D]]
-	[[Category: Schaffner, I]]	+	[[Category: Schaffner I]]
-	~~[[Category: Chlorite dismutase]]~~	+
-	~~[[Category: Cyanobacteria]]~~	+
-	~~[[Category: Ferredoxin-like fold]]~~	+
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 10:50, 2 October 2019

OCA — Wed, 02 Oct 2019 10:50:32 GMT

←Older revision		Revision as of 10:50, 2 October 2019
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	==Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 in complex with isothiocyanate==		==Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 in complex with isothiocyanate==
-	<StructureSection load='5k90' size='340' side='right' caption='[[5k90]], [[Resolution\|resolution]] 1.28Å' scene=''>	+	<StructureSection load='5k90' size='340' side='right'caption='[[5k90]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5k90]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyap4 Cyap4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K90 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5k90]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyap4 Cyap4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K90 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Cyap4]]		[[Category: Cyap4]]
		+	[[Category: Large Structures]]
	[[Category: Djinovic-Carugo, K]]		[[Category: Djinovic-Carugo, K]]
	[[Category: Mlynek, G]]		[[Category: Mlynek, G]]

OCA at 06:55, 31 January 2018

OCA — Wed, 31 Jan 2018 06:55:10 GMT

←Older revision		Revision as of 06:55, 31 January 2018
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k90 OCA], [http://pdbe.org/5k90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k90 RCSB], [http://www.ebi.ac.uk/pdbsum/5k90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k90 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k90 OCA], [http://pdbe.org/5k90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k90 RCSB], [http://www.ebi.ac.uk/pdbsum/5k90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k90 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The heme enzyme chlorite dismutase (Cld) catalyzes the degradation of chlorite to chloride and dioxygen. Although structure and steady-state kinetics of Clds have been elucidated, many questions remain (e.g., the mechanism of chlorite cleavage and the pH dependence of the reaction). Here, we present high-resolution X-ray crystal structures of a dimeric Cld at pH 6.5 and 8.5, its fluoride and isothiocyanate complexes and the neutron structure at pH 9.0 together with the pH dependence of the Fe(III)/Fe(II) couple, and the UV-vis and resonance Raman spectral features. We demonstrate that the distal Arg127 cannot act as proton acceptor and is fully ionized even at pH 9.0 ruling out its proposed role in dictating the pH dependence of chlorite degradation. Stopped-flow studies show that (i) Compound I and hypochlorite do not recombine and (ii) Compound II is the immediately formed redox intermediate that dominates during turnover. Homolytic cleavage of chlorite is proposed.
		+
		+	Molecular Mechanism of Enzymatic Chlorite Detoxification: Insights from Structural and Kinetic Studies.,Schaffner I, Mlynek G, Flego N, Puhringer D, Libiseller-Egger J, Coates L, Hofbauer S, Bellei M, Furtmuller PG, Battistuzzi G, Smulevich G, Djinovic-Carugo K, Obinger C ACS Catal. 2017 Nov 3;7(11):7962-7976. doi: 10.1021/acscatal.7b01749. Epub 2017, Oct 13. PMID:29142780<ref>PMID:29142780</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5k90" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:36, 16 November 2017

OCA — Thu, 16 Nov 2017 08:36:23 GMT

←Older revision		Revision as of 08:36, 16 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5k90 ~~is ON HOLD~~	+	==Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 in complex with isothiocyanate==
-		+	<StructureSection load='5k90' size='340' side='right' caption='[[5k90]], [[Resolution\|resolution]] 1.28Å' scene=''>
-	~~Authors~~: ~~Puehringer, D~~., ~~Schaffner~~, I., ~~Mlynek~~, G., ~~Obinger~~, C., ~~Djinovic~~-~~Carugo, K~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5k90]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyap4 Cyap4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K90 FirstGlance]. <br>
-	~~Description: Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 in complex with isothiocyanate~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IS8:isothiocyanate'>IS8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cyan7425_1434 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395961 CYAP4])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k90 OCA], [http://pdbe.org/5k90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k90 RCSB], [http://www.ebi.ac.uk/pdbsum/5k90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k90 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cyap4]]
	[[Category: Djinovic-Carugo, K]]		[[Category: Djinovic-Carugo, K]]
	[[Category: Mlynek, G]]		[[Category: Mlynek, G]]
		+	[[Category: Obinger, C]]
	[[Category: Puehringer, D]]		[[Category: Puehringer, D]]
	[[Category: Schaffner, I]]		[[Category: Schaffner, I]]
-	[[Category: ~~Obinger, C~~]]	+	[[Category: Chlorite dismutase]]
		+	[[Category: Cyanobacteria]]
		+	[[Category: Ferredoxin-like fold]]
		+	[[Category: Heme]]
		+	[[Category: Oxidoreductase]]

OCA at 10:13, 9 March 2017

OCA — Thu, 09 Mar 2017 10:13:01 GMT

←Older revision		Revision as of 10:13, 9 March 2017
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	[[Category: Djinovic-Carugo, K]]		[[Category: Djinovic-Carugo, K]]
	[[Category: Mlynek, G]]		[[Category: Mlynek, G]]
		+	[[Category: Puehringer, D]]
	[[Category: Schaffner, I]]		[[Category: Schaffner, I]]
	[[Category: Obinger, C]]		[[Category: Obinger, C]]
-	[[Category: Puehringer, D]]

OCA at 15:14, 9 December 2016

OCA — Fri, 09 Dec 2016 15:14:18 GMT

←Older revision		Revision as of 15:14, 9 December 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5k90 is ON HOLD ~~until Paper Publication~~	+	The entry 5k90 is ON HOLD

	Authors: Puehringer, D., Schaffner, I., Mlynek, G., Obinger, C., Djinovic-Carugo, K.		Authors: Puehringer, D., Schaffner, I., Mlynek, G., Obinger, C., Djinovic-Carugo, K.

-	Description: Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 in complex with ~~thiocyanate~~	+	Description: Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 in complex with isothiocyanate
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Djinovic-Carugo, K]]		[[Category: Djinovic-Carugo, K]]

OCA: Protected "5k90" [edit=sysop:move=sysop]

OCA — Mon, 20 Jun 2016 13:34:39 GMT

Protected "5k90" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:34, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5k90 is ON HOLD until Paper Publication Authors: Puehringer, D., Schaffner, I., Mlynek, G., Obinger, C., Djinovic-Carugo, K. Description: Crystal s...

OCA — Mon, 20 Jun 2016 13:34:38 GMT

New page: '''Unreleased structure''' The entry 5k90 is ON HOLD until Paper Publication Authors: Puehringer, D., Schaffner, I., Mlynek, G., Obinger, C., Djinovic-Carugo, K. Description: Crystal s...

New page

'''Unreleased structure'''

The entry 5k90 is ON HOLD until Paper Publication

Authors: Puehringer, D., Schaffner, I., Mlynek, G., Obinger, C., Djinovic-Carugo, K.

Description: Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 in complex with thiocyanate
[[Category: Unreleased Structures]]
[[Category: Djinovic-Carugo, K]]
[[Category: Mlynek, G]]
[[Category: Schaffner, I]]
[[Category: Obinger, C]]
[[Category: Puehringer, D]]