5k94 - Revision history

OCA at 09:55, 27 September 2023

OCA — Wed, 27 Sep 2023 09:55:22 GMT

←Older revision		Revision as of 09:55, 27 September 2023
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	<StructureSection load='5k94' size='340' side='right'caption='[[5k94]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5k94' size='340' side='right'caption='[[5k94]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k94]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K94 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5K94 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k94]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K94 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=B3P:2~~-[~~3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO~~]-2~~-HYDROXYMETHYL-PROPANE-~~1~~,3-DIOL'>B3P</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~malE, Z5632, ECs5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83334 ECO57]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5k94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k94 OCA], [~~http~~://pdbe.org/5k94 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5k94 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5k94 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5k94 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k94 OCA], [https://pdbe.org/5k94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k94 RCSB], [https://www.ebi.ac.uk/pdbsum/5k94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k94 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~MALE_ECO57 MALE_ECO57]~~] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides ~~(By similarity)~~.	+	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/SECA_ECOLI SECA_ECOLI] Required for protein export, interacts with the SecYEG preprotein conducting channel. SecA has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.<ref>PMID:15140892</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eco57~~]]	+	[[Category: Escherichia coli O157:H7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ghonaim, N]]	+	[[Category: Ghonaim N]]
-	[[Category: Hackett, J]]	+	[[Category: Hackett J]]
-	[[Category: Shilton~~, B H]]~~	+	[[Category: Shilton BH]]
-	~~[[Category: Mbp chimera]]~~	+
-	~~[[Category: Ppxd]]~~	+
-	~~[[Category: Preprotein cross-linking domain]]~~	+
-	~~[[Category: Preprotein translocase]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Seca~~]]	+

OCA at 08:08, 8 January 2020

OCA — Wed, 08 Jan 2020 08:08:50 GMT

←Older revision		Revision as of 08:08, 8 January 2020
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	==Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase==		==Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase==
-	<StructureSection load='5k94' size='340' side='right' caption='[[5k94]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5k94' size='340' side='right'caption='[[5k94]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5k94]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K94 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5k94]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K94 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Eco57]]		[[Category: Eco57]]
		+	[[Category: Large Structures]]
	[[Category: Ghonaim, N]]		[[Category: Ghonaim, N]]
	[[Category: Hackett, J]]		[[Category: Hackett, J]]

OCA at 20:05, 19 September 2018

OCA — Wed, 19 Sep 2018 20:05:15 GMT

←Older revision		Revision as of 20:05, 19 September 2018
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).		[[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We coupled peptides from a CNBr digest of signal-sequenceless maltose-binding protein (MBP) to a surface plasmon resonance chip. SecA-N95, SecA-N68, and SecA-DM (which consists of only the DEAD Motor domains NBD1 and NBD2) bound to the immobilized peptides; ADP weakened the binding. SecA-DM, which lacks the 'preprotein cross-linking domain' (PPXD), displayed the most extensive binding, while an MBP-PPXD chimera showed no binding, demonstrating that the PPXD does not contribute to the binding. We characterized the sequence specificity using oriented peptide libraries; these results enabled synthesis of a 20-residue peptide that was used to recapitulate the results obtained with MBP-derived peptides. This study shows that there is a promiscuous and nucleotide-modulated peptide-binding site in the DEAD Motor domains of SecA.
		+
		+	Characterization of a polypeptide-binding site in the DEAD Motor of the SecA ATPase.,Khalili Yazdi A, Namjoshi S, Hackett J, Ghonaim N, Shilton BH FEBS Lett. 2017 Oct;591(20):3378-3390. doi: 10.1002/1873-3468.12832. Epub 2017, Sep 12. PMID:28862749<ref>PMID:28862749</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5k94" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 18:10, 15 November 2017

OCA — Wed, 15 Nov 2017 18:10:43 GMT

←Older revision		Revision as of 18:10, 15 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5k94 is ~~ON HOLD until Paper Publication~~	+	==Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase==
-		+	<StructureSection load='5k94' size='340' side='right' caption='[[5k94]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors~~: ~~Shilton~~, B.H., ~~Hackett~~, J., ~~Ghonaim~~, N.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5k94]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K94 FirstGlance]. <br>
-	~~Description~~: ~~Deletion-Insertion Chimera of MBP with~~ the ~~Preprotein Cross~~-~~Linking Domain of~~ the ~~SecA ATPase~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE, Z5632, ECs5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k94 OCA], [http://pdbe.org/5k94 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k94 RCSB], [http://www.ebi.ac.uk/pdbsum/5k94 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k94 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Eco57]]
	[[Category: Ghonaim, N]]		[[Category: Ghonaim, N]]
	[[Category: Hackett, J]]		[[Category: Hackett, J]]
-	[[Category: Shilton, B.H]]	+	[[Category: Shilton, B H]]
		+	[[Category: Mbp chimera]]
		+	[[Category: Ppxd]]
		+	[[Category: Preprotein cross-linking domain]]
		+	[[Category: Preprotein translocase]]
		+	[[Category: Protein transport]]
		+	[[Category: Seca]]

OCA at 00:08, 13 July 2016

OCA — Wed, 13 Jul 2016 00:08:09 GMT

←Older revision		Revision as of 00:08, 13 July 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5k94 is ON HOLD	+	The entry 5k94 is ON HOLD until Paper Publication

	Authors: Shilton, B.H., Hackett, J., Ghonaim, N.		Authors: Shilton, B.H., Hackett, J., Ghonaim, N.

OCA at 21:15, 23 June 2016

OCA — Thu, 23 Jun 2016 21:15:06 GMT

←Older revision		Revision as of 21:15, 23 June 2016
Line 3:		Line 3:
	The entry 5k94 is ON HOLD		The entry 5k94 is ON HOLD

-	Authors: Shilton, BH, Hackett, J, Ghonaim, N	+	Authors: Shilton, B.H., Hackett, J., Ghonaim, N.

	Description: Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase		Description: Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Shilton, Bh~~, Hackett, J, ~~Ghonaim, N~~]]	+	[[Category: Ghonaim, N]]
		+	[[Category: Hackett, J]]
		+	[[Category: Shilton, B.H]]

OCA: Protected "5k94" [edit=sysop:move=sysop]

OCA — Mon, 20 Jun 2016 13:35:03 GMT

Protected "5k94" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:35, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5k94 is ON HOLD Authors: Shilton, BH, Hackett, J, Ghonaim, N Description: Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domai...

OCA — Mon, 20 Jun 2016 13:35:02 GMT

New page: '''Unreleased structure''' The entry 5k94 is ON HOLD Authors: Shilton, BH, Hackett, J, Ghonaim, N Description: Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domai...

New page

'''Unreleased structure'''

The entry 5k94 is ON HOLD

Authors: Shilton, BH, Hackett, J, Ghonaim, N

Description: Deletion-Insertion Chimera of MBP with the Preprotein Cross-Linking Domain of the SecA ATPase
[[Category: Unreleased Structures]]
[[Category: Shilton, Bh, Hackett, J, Ghonaim, N]]