5kaw - Revision history

OCA at 13:05, 1 March 2024

OCA — Fri, 01 Mar 2024 13:05:11 GMT

←Older revision		Revision as of 13:05, 1 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A0H3JRN6_STAAN A0A0H3JRN6_STAAN]		[https://www.uniprot.org/uniprot/A0A0H3JRN6_STAAN A0A0H3JRN6_STAAN]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Multidrug resistance (MDR) refers to the acquired ability of cells to tolerate a broad range of toxic compounds. One mechanism cells employ is to increase the level of expression of efflux pumps for the expulsion of xenobiotics. A key feature uniting efflux-related mechanisms is multidrug (MD) recognition, either by efflux pumps themselves or by their transcriptional regulators. However, models describing MD binding by MDR effectors are incomplete, underscoring the importance of studies focused on the recognition elements and key motifs that dictate polyspecific binding. One such motif is the GyrI-like domain, which is found in several MDR proteins and is postulated to have been adapted for small-molecule binding and signaling. Here we report the solution binding properties and crystal structures of two proteins containing GyrI-like domains, SAV2435 and CTR107, bound to various ligands. Furthermore, we provide a comparison with deposited crystal structures of GyrI-like proteins, revealing key features of GyrI-like domains that not only support polyspecific binding but also are conserved among GyrI-like domains. Together, our studies suggest that GyrI-like domains perform evolutionarily conserved functions connected to multidrug binding and highlight the utility of these types of studies for elucidating mechanisms of MDR.
-
-	Solution Binding and Structural Analyses Reveal Potential Multidrug Resistance Functions for SAV2435 and CTR107 and Other GyrI-like Proteins.,Moreno A, Froehlig JR, Bachas S, Gunio D, Alexander T, Vanya A, Wade H Biochemistry. 2016 Aug 30;55(34):4850-63. doi: 10.1021/acs.biochem.6b00651. Epub , 2016 Aug 18. PMID:27505298<ref>PMID:27505298</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5kaw" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:44, 27 September 2023

OCA — Wed, 27 Sep 2023 10:44:11 GMT

←Older revision		Revision as of 10:44, 27 September 2023
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	<StructureSection load='5kaw' size='340' side='right'caption='[[5kaw]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='5kaw' size='340' side='right'caption='[[5kaw]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kaw]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staan Staan~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KAW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kaw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_N315 Staphylococcus aureus subsp. aureus N315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KAW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene>, <scene name='pdbligand=RHQ:RHODAMINE+6G'>RHQ</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.86Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SA2223 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene>, <scene name='pdbligand=RHQ:RHODAMINE+6G'>RHQ</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kaw OCA], [~~http~~://pdbe.org/5kaw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kaw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kaw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kaw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kaw OCA], [https://pdbe.org/5kaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kaw RCSB], [https://www.ebi.ac.uk/pdbsum/5kaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kaw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H3JRN6_STAAN A0A0H3JRN6_STAAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staan~~]]	+	[[Category: Staphylococcus aureus subsp. aureus N315]]
-	[[Category: Moreno, A]]	+	[[Category: Moreno A]]
-	[[Category: Wade, H]]	+	[[Category: Wade H]]
-	~~[[Category: Gyri-like domatin]]~~	+
-	~~[[Category: Multi-drug recognition]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 07:43, 27 November 2019

OCA — Wed, 27 Nov 2019 07:43:15 GMT

←Older revision		Revision as of 07:43, 27 November 2019
Line 1:		Line 1:

	==The structure of SAV2435 bound to TETRAPHENYLPHOSPHONIUM and RHODAMINE 6G==		==The structure of SAV2435 bound to TETRAPHENYLPHOSPHONIUM and RHODAMINE 6G==
-	<StructureSection load='5kaw' size='340' side='right' caption='[[5kaw]], [[Resolution\|resolution]] 1.86Å' scene=''>	+	<StructureSection load='5kaw' size='340' side='right'caption='[[5kaw]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kaw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kaw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staan Staan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KAW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene>, <scene name='pdbligand=RHQ:RHODAMINE+6G'>RHQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene>, <scene name='pdbligand=RHQ:RHODAMINE+6G'>RHQ</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SA2223 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kaw OCA], [http://pdbe.org/5kaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kaw RCSB], [http://www.ebi.ac.uk/pdbsum/5kaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kaw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kaw OCA], [http://pdbe.org/5kaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kaw RCSB], [http://www.ebi.ac.uk/pdbsum/5kaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kaw ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Staan]]
	[[Category: Moreno, A]]		[[Category: Moreno, A]]
	[[Category: Wade, H]]		[[Category: Wade, H]]

OCA at 05:38, 9 September 2016

OCA — Fri, 09 Sep 2016 05:38:33 GMT

←Older revision		Revision as of 05:38, 9 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5kaw is ~~ON HOLD until Paper~~ Publication	+	==The structure of SAV2435 bound to TETRAPHENYLPHOSPHONIUM and RHODAMINE 6G==
		+	<StructureSection load='5kaw' size='340' side='right' caption='[[5kaw]], [[Resolution\|resolution]] 1.86Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kaw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KAW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene>, <scene name='pdbligand=RHQ:RHODAMINE+6G'>RHQ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kaw OCA], [http://pdbe.org/5kaw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kaw RCSB], [http://www.ebi.ac.uk/pdbsum/5kaw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kaw ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multidrug resistance (MDR) refers to the acquired ability of cells to tolerate a broad range of toxic compounds. One mechanism cells employ is to increase the level of expression of efflux pumps for the expulsion of xenobiotics. A key feature uniting efflux-related mechanisms is multidrug (MD) recognition, either by efflux pumps themselves or by their transcriptional regulators. However, models describing MD binding by MDR effectors are incomplete, underscoring the importance of studies focused on the recognition elements and key motifs that dictate polyspecific binding. One such motif is the GyrI-like domain, which is found in several MDR proteins and is postulated to have been adapted for small-molecule binding and signaling. Here we report the solution binding properties and crystal structures of two proteins containing GyrI-like domains, SAV2435 and CTR107, bound to various ligands. Furthermore, we provide a comparison with deposited crystal structures of GyrI-like proteins, revealing key features of GyrI-like domains that not only support polyspecific binding but also are conserved among GyrI-like domains. Together, our studies suggest that GyrI-like domains perform evolutionarily conserved functions connected to multidrug binding and highlight the utility of these types of studies for elucidating mechanisms of MDR.

-	~~Authors~~:	+	Solution Binding and Structural Analyses Reveal Potential Multidrug Resistance Functions for SAV2435 and CTR107 and Other GyrI-like Proteins.,Moreno A, Froehlig JR, Bachas S, Gunio D, Alexander T, Vanya A, Wade H Biochemistry. 2016 Aug 30;55(34):4850-63. doi: 10.1021/acs.biochem.6b00651. Epub , 2016 Aug 18. PMID:27505298<ref>PMID:27505298</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5kaw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Moreno, A]]
		+	[[Category: Wade, H]]
		+	[[Category: Gyri-like domatin]]
		+	[[Category: Multi-drug recognition]]
		+	[[Category: Unknown function]]

OCA at 09:08, 14 July 2016

OCA — Thu, 14 Jul 2016 09:08:05 GMT

←Older revision		Revision as of 09:08, 14 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kaw is ON HOLD	+	The entry 5kaw is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5kaw" [edit=sysop:move=sysop]

OCA — Mon, 20 Jun 2016 13:39:21 GMT

Protected "5kaw" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:39, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5kaw is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Mon, 20 Jun 2016 13:39:19 GMT

New page: '''Unreleased structure''' The entry 5kaw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5kaw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]