5kej - Revision history

OCA at 09:59, 27 September 2023

OCA — Wed, 27 Sep 2023 09:59:20 GMT

←Older revision		Revision as of 09:59, 27 September 2023
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	<StructureSection load='5kej' size='340' side='right'caption='[[5kej]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='5kej' size='340' side='right'caption='[[5kej]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kej]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Manin Manin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5KEJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kej]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mangifera_indica Mangifera indica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KEJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GTX:S~~-~~HEXYLGLUTATHIONE'>GTX</scene>~~, ~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5kej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kej OCA], [~~http~~://pdbe.org/5kej PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kej RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kej PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kej ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kej OCA], [https://pdbe.org/5kej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kej RCSB], [https://www.ebi.ac.uk/pdbsum/5kej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kej ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1P8NWC2_MANIN A0A1P8NWC2_MANIN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Glutathione transferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Manin~~]]	+	[[Category: Mangifera indica]]
-	[[Category: Hernandez-Paredes, J]]	+	[[Category: Hernandez-Paredes J]]
-	[[Category: Lopez-Zavala, A]]	+	[[Category: Lopez-Zavala A]]
-	[[Category: Serrano-Posada, H]]	+	[[Category: Serrano-Posada H]]
-	[[Category: Sotelo-Mundo, R]]	+	[[Category: Sotelo-Mundo R]]
-	[[Category: Valenzuela-Chavira, I]]	+	[[Category: Valenzuela-Chavira I]]
-	~~[[Category: Detoxification]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 15:24, 8 July 2020

OCA — Wed, 08 Jul 2020 15:24:04 GMT

←Older revision		Revision as of 15:24, 8 July 2020
Line 1:		Line 1:

	==Crystallographic structure of the Tau class glutathione S-transferase MiGSTU in complex with S-hexyl-glutathione==		==Crystallographic structure of the Tau class glutathione S-transferase MiGSTU in complex with S-hexyl-glutathione==
-	<StructureSection load='5kej' size='340' side='right' caption='[[5kej]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='5kej' size='340' side='right'caption='[[5kej]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kej]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KEJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kej]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Manin Manin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KEJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kej OCA], [http://pdbe.org/5kej PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kej RCSB], [http://www.ebi.ac.uk/pdbsum/5kej PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kej ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5kej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kej OCA], [http://pdbe.org/5kej PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kej RCSB], [http://www.ebi.ac.uk/pdbsum/5kej PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kej ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5kej" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5kej" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
		+	[[Category: Large Structures]]
		+	[[Category: Manin]]
	[[Category: Hernandez-Paredes, J]]		[[Category: Hernandez-Paredes, J]]
	[[Category: Lopez-Zavala, A]]		[[Category: Lopez-Zavala, A]]

OCA at 17:53, 1 February 2017

OCA — Wed, 01 Feb 2017 17:53:43 GMT

←Older revision		Revision as of 17:53, 1 February 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5kej is ~~ON HOLD until Jun 09 2018~~	+	==Crystallographic structure of the Tau class glutathione S-transferase MiGSTU in complex with S-hexyl-glutathione==
		+	<StructureSection load='5kej' size='340' side='right' caption='[[5kej]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kej]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KEJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kej OCA], [http://pdbe.org/5kej PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kej RCSB], [http://www.ebi.ac.uk/pdbsum/5kej PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kej ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We studied a mango glutathione S-transferase (GST) (Mangifera indica) bound to glutathione (GSH) and S-hexyl glutathione (GSX). This GST Tau class (MiGSTU) had a molecular mass of 25.5 kDa. MiGSTU Michaelis-Menten kinetic constants were determined for their substrates obtaining a Km, Vmax and kcat for CDNB of 0.792 mM, 80.58 mM min-1 and 68.49 s-1 respectively and 0.693 mM, 105.32 mM min-1 and 89.57 s-1, for reduced GSH respectively. MiGSTU had a micromolar affinity towards GSH (5.2 muM) or GSX (7.8 muM). The crystal structure of the MiGSTU in apo or bound to GSH or GSX generated a model that explains the thermodynamic signatures of binding and showed the importance of enthalpic-entropic compensation in ligand binding to Tau-class GST enzymes.

-	~~Authors~~: Valenzuela-Chavira, I., Serrano-Posada, H., Lopez-Zavala, A., Hernandez-Paredes, J., Sotelo-Mundo, R.	+	Insights into ligand binding to a glutathione S-transferase from mango: Structure, thermodynamics and kinetics.,Valenzuela-Chavira I, Contreras-Vergara CA, Arvizu-Flores AA, Serrano-Posada H, Lopez-Zavala AA, Garcia-Orozco KD, Hernandez-Paredes J, Rudino-Pinera E, Stojanoff V, Sotelo-Mundo RR, Islas-Osuna MA Biochimie. 2017 Jan 16. pii: S0300-9084(16)30283-8. doi:, 10.1016/j.biochi.2017.01.005. PMID:28104507<ref>PMID:28104507</ref>

-	~~Description: Crystallographic structure~~ of the ~~Tau~~ class ~~glutathione S~~-~~transferase MiGSTU in complex with S~~-~~hexyl-glutathione~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Valenzuela-Chavira, I]]~~	+	<div class="pdbe-citations 5kej" style="background-color:#fffaf0;"></div>
-	~~[[Category: Lopez-Zavala, A]]~~	+	== References ==
-	[[Category: ~~Sotelo-Mundo, R~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glutathione transferase]]
	[[Category: Hernandez-Paredes, J]]		[[Category: Hernandez-Paredes, J]]
		+	[[Category: Lopez-Zavala, A]]
	[[Category: Serrano-Posada, H]]		[[Category: Serrano-Posada, H]]
		+	[[Category: Sotelo-Mundo, R]]
		+	[[Category: Valenzuela-Chavira, I]]
		+	[[Category: Detoxification]]
		+	[[Category: Transferase]]

OCA at 00:18, 13 July 2016

OCA — Wed, 13 Jul 2016 00:18:19 GMT

←Older revision		Revision as of 00:18, 13 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kej is ON HOLD	+	The entry 5kej is ON HOLD until Jun 09 2018

	Authors: Valenzuela-Chavira, I., Serrano-Posada, H., Lopez-Zavala, A., Hernandez-Paredes, J., Sotelo-Mundo, R.		Authors: Valenzuela-Chavira, I., Serrano-Posada, H., Lopez-Zavala, A., Hernandez-Paredes, J., Sotelo-Mundo, R.

OCA at 21:29, 23 June 2016

OCA — Thu, 23 Jun 2016 21:29:39 GMT

←Older revision		Revision as of 21:29, 23 June 2016
Line 3:		Line 3:
	The entry 5kej is ON HOLD		The entry 5kej is ON HOLD

-	Authors: Valenzuela-Chavira, I, Serrano-Posada, H, Lopez-Zavala, A, Hernandez-Paredes, J, Sotelo-Mundo, R	+	Authors: Valenzuela-Chavira, I., Serrano-Posada, H., Lopez-Zavala, A., Hernandez-Paredes, J., Sotelo-Mundo, R.

-	Description: Crystallographic structure of the Tau class glutathione S-transferase MiGSTU in complex with S-hexyl-glutathione.	+	Description: Crystallographic structure of the Tau class glutathione S-transferase MiGSTU in complex with S-hexyl-glutathione
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Valenzuela-Chavira, I~~, Serrano-Posada, H,~~ Lopez-Zavala, A, Hernandez-Paredes, J~~, Sotelo~~-~~Mundo~~, R]]	+	[[Category: Valenzuela-Chavira, I]]
		+	[[Category: Lopez-Zavala, A]]
		+	[[Category: Sotelo-Mundo, R]]
		+	[[Category: Hernandez-Paredes, J]]
		+	[[Category: Serrano-Posada, H]]

OCA: Protected "5kej" [edit=sysop:move=sysop]

OCA — Mon, 20 Jun 2016 13:50:31 GMT

Protected "5kej" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:50, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5kej is ON HOLD Authors: Valenzuela-Chavira, I, Serrano-Posada, H, Lopez-Zavala, A, Hernandez-Paredes, J, Sotelo-Mundo, R Description: Crystallogra...

OCA — Mon, 20 Jun 2016 13:50:30 GMT

New page: '''Unreleased structure''' The entry 5kej is ON HOLD Authors: Valenzuela-Chavira, I, Serrano-Posada, H, Lopez-Zavala, A, Hernandez-Paredes, J, Sotelo-Mundo, R Description: Crystallogra...

New page

'''Unreleased structure'''

The entry 5kej is ON HOLD

Authors: Valenzuela-Chavira, I, Serrano-Posada, H, Lopez-Zavala, A, Hernandez-Paredes, J, Sotelo-Mundo, R

Description: Crystallographic structure of the Tau class glutathione S-transferase MiGSTU in complex with S-hexyl-glutathione.
[[Category: Unreleased Structures]]
[[Category: Valenzuela-Chavira, I, Serrano-Posada, H, Lopez-Zavala, A, Hernandez-Paredes, J, Sotelo-Mundo, R]]