5kho - Revision history

OCA at 10:01, 27 September 2023

OCA — Wed, 27 Sep 2023 10:01:09 GMT

←Older revision		Revision as of 10:01, 27 September 2023
Line 3:		Line 3:
	<StructureSection load='5kho' size='340' side='right'caption='[[5kho]], [[Resolution\|resolution]] 2.78Å' scene=''>		<StructureSection load='5kho' size='340' side='right'caption='[[5kho]], [[Resolution\|resolution]] 2.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kho]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5KHO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kho]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KHO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.78Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5khq\|5khq]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5kho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kho OCA], [~~http~~://pdbe.org/5kho PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kho RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kho PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kho ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kho OCA], [https://pdbe.org/5kho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kho RCSB], [https://www.ebi.ac.uk/pdbsum/5kho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kho ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RAIN_HUMAN RAIN_HUMAN]] Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy.<ref>PMID:15031288</ref> <ref>PMID:22354037</ref> [[http://www.uniprot.org/uniprot/RAP1B_HUMAN RAP1B_HUMAN]] GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction.<ref>PMID:20332120</ref> <ref>PMID:18660803</ref>	+	[https://www.uniprot.org/uniprot/RAIN_HUMAN RAIN_HUMAN] Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy.<ref>PMID:15031288</ref> <ref>PMID:22354037</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gingras~~, A R]]~~	+	[[Category: Gingras AR]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Rap1b]]~~	+
-	~~[[Category: Ras-association domain]]~~	+
-	~~[[Category: Rasip1]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 15:25, 8 July 2020

OCA — Wed, 08 Jul 2020 15:25:46 GMT

←Older revision		Revision as of 15:25, 8 July 2020
Line 1:		Line 1:

	==Rasip1 RA domain in complex with Rap1B==		==Rasip1 RA domain in complex with Rap1B==
-	<StructureSection load='5kho' size='340' side='right' caption='[[5kho]], [[Resolution\|resolution]] 2.78Å' scene=''>	+	<StructureSection load='5kho' size='340' side='right'caption='[[5kho]], [[Resolution\|resolution]] 2.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kho]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KHO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kho]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KHO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5khq\|5khq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5khq\|5khq]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kho OCA], [http://pdbe.org/5kho PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kho RCSB], [http://www.ebi.ac.uk/pdbsum/5kho PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kho ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5kho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kho OCA], [http://pdbe.org/5kho PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kho RCSB], [http://www.ebi.ac.uk/pdbsum/5kho PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kho ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5kho" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5kho" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gingras, A R]]		[[Category: Gingras, A R]]
	[[Category: Complex]]		[[Category: Complex]]

OCA at 07:38, 9 March 2017

OCA — Thu, 09 Mar 2017 07:38:16 GMT

←Older revision		Revision as of 07:38, 9 March 2017
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RAIN_HUMAN RAIN_HUMAN]] Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy.<ref>PMID:15031288</ref> <ref>PMID:22354037</ref> [[http://www.uniprot.org/uniprot/RAP1B_HUMAN RAP1B_HUMAN]] GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction.<ref>PMID:20332120</ref> <ref>PMID:18660803</ref>		[[http://www.uniprot.org/uniprot/RAIN_HUMAN RAIN_HUMAN]] Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy.<ref>PMID:15031288</ref> <ref>PMID:22354037</ref> [[http://www.uniprot.org/uniprot/RAP1B_HUMAN RAP1B_HUMAN]] GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction.<ref>PMID:20332120</ref> <ref>PMID:18660803</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ras-interacting protein 1 (Rasip1) is an endothelial-specific Rap1 and Ras effector, important for vascular development and angiogenesis. Here, we report the crystal structure of the Rasip1 RA domain (RRA) alone, revealing the basis of dimerization, and in complex with Rap1 at 2.8 A resolution. In contrast to most RA domains, RRA formed a dimer that can bind two Rap1 (KD = 0.9 muM) or Ras (KD = 2.2 muM) molecules. We solved the Rap1-RRA complex and found that Rasip1 binds Rap1 in the Switch I region, and Rap1 binding induces few conformation changes to Rasip1 stabilizing a beta strand and an unstructured loop. Our data explain how Rasip1 can act as a Rap1 and Ras effector and show that Rasip1 defines a subgroup of dimeric RA domains that could mediate cooperative binding to membrane-associated Ras superfamily members.
		+
		+	Structural Basis of Dimeric Rasip1 RA Domain Recognition of the Ras Subfamily of GTP-Binding Proteins.,Gingras AR, Puzon-McLaughlin W, Bobkov AA, Ginsberg MH Structure. 2016 Dec 6;24(12):2152-2162. doi: 10.1016/j.str.2016.10.001. Epub 2016, Nov 10. PMID:27839947<ref>PMID:27839947</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5kho" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:00, 20 October 2016

OCA — Thu, 20 Oct 2016 01:00:07 GMT

←Older revision		Revision as of 01:00, 20 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5kho is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Rasip1 RA domain in complex with Rap1B==
-		+	<StructureSection load='5kho' size='340' side='right' caption='[[5kho]], [[Resolution\|resolution]] 2.78Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5kho]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KHO FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5khq\|5khq]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kho OCA], [http://pdbe.org/5kho PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kho RCSB], [http://www.ebi.ac.uk/pdbsum/5kho PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kho ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RAIN_HUMAN RAIN_HUMAN]] Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy.<ref>PMID:15031288</ref> <ref>PMID:22354037</ref> [[http://www.uniprot.org/uniprot/RAP1B_HUMAN RAP1B_HUMAN]] GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction.<ref>PMID:20332120</ref> <ref>PMID:18660803</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gingras, A R]]
		+	[[Category: Complex]]
		+	[[Category: Rap1b]]
		+	[[Category: Ras-association domain]]
		+	[[Category: Rasip1]]
		+	[[Category: Signaling protein]]

OCA at 00:22, 13 July 2016

OCA — Wed, 13 Jul 2016 00:22:02 GMT

←Older revision		Revision as of 00:22, 13 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kho is ON HOLD	+	The entry 5kho is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5kho" [edit=sysop:move=sysop]

OCA — Thu, 23 Jun 2016 21:37:59 GMT

Protected "5kho" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:37, 23 June 2016

OCA: New page: '''Unreleased structure''' The entry 5kho is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 23 Jun 2016 21:37:58 GMT

New page: '''Unreleased structure''' The entry 5kho is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5kho is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]