5ki0 - Revision history

OCA at 10:13, 14 June 2023

OCA — Wed, 14 Jun 2023 10:13:19 GMT

←Older revision		Revision as of 10:13, 14 June 2023
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	==NMR structure of human antimicrobial peptide KAMP-19==		==NMR structure of human antimicrobial peptide KAMP-19==
-	<StructureSection load='5ki0' size='340' side='right'caption='[[5ki0~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='5ki0' size='340' side='right'caption='[[5ki0]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ki0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KI0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KI0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ki0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KI0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ki0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ki0 OCA], [~~http~~://pdbe.org/5ki0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ki0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ki0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ki0 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ki0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ki0 OCA], [https://pdbe.org/5ki0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ki0 RCSB], [https://www.ebi.ac.uk/pdbsum/5ki0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ki0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/K2C6A_HUMAN K2C6A_HUMAN] Pachyonychia congenita. The disease is caused by variants affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/K2C6A_HUMAN K2C6A_HUMAN] Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.[UniProtKB:P50446]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Wang, G]]	+	[[Category: Wang G]]
-	~~[[Category: Antimicrobial protein]]~~	+
-	~~[[Category: Keratin 6a fragment]]~~	+
-	~~[[Category: Non-alpha beta structure~~]]	+

OCA at 16:28, 11 December 2019

OCA — Wed, 11 Dec 2019 16:28:20 GMT

←Older revision		Revision as of 16:28, 11 December 2019
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	==NMR structure of human antimicrobial peptide KAMP-19==		==NMR structure of human antimicrobial peptide KAMP-19==
-	<StructureSection load='5ki0' size='340' side='right' caption='[[5ki0]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='5ki0' size='340' side='right'caption='[[5ki0]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ki0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KI0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ki0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KI0 FirstGlance]. <br>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Wang, G]]		[[Category: Wang, G]]
	[[Category: Antimicrobial protein]]		[[Category: Antimicrobial protein]]
	[[Category: Keratin 6a fragment]]		[[Category: Keratin 6a fragment]]
	[[Category: Non-alpha beta structure]]		[[Category: Non-alpha beta structure]]

OCA at 14:12, 22 December 2016

OCA — Thu, 22 Dec 2016 14:12:20 GMT

←Older revision		Revision as of 14:12, 22 December 2016
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ki0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ki0 OCA], [http://pdbe.org/5ki0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ki0 RCSB], [http://www.ebi.ac.uk/pdbsum/5ki0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ki0 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ki0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ki0 OCA], [http://pdbe.org/5ki0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ki0 RCSB], [http://www.ebi.ac.uk/pdbsum/5ki0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ki0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antibiotic resistance is a pressing global health problem that threatens millions of lives each year. Natural antimicrobial peptides and their synthetic derivatives, including peptoids and peptidomimetics, are promising candidates as novel antibiotics. Recently, the C-terminal glycine-rich fragments of human epithelial keratin 6A were found to have bactericidal and cytoprotective activities. Here, we used an improved 2-dimensional NMR method coupled with a new protocol for structural refinement by low temperature simulated annealing to characterize the solution structure of these kerain-derived antimicrobial peptides (KAMPs). Two specific KAMPs in complex with membrane mimicking sodium dodecyl sulfate (SDS) micelles displayed amphipathic conformations with only local bends and turns, and a central 10-residue glycine-rich hydrophobic strip that is central to bactericidal activity. To our knowledge, this is the first report of non-alphabeta structure for human antimicrobial peptides. Direct observation of Staphylococcus aureus and Pseudomonas aeruginosa by scanning and transmission electron microscopy showed that KAMPs deformed bacterial cell envelopes and induced pore formation. Notably, in competitive binding experiments, KAMPs demonstrated binding affinities to LPS and LTA that did not correlate with their bactericidal activities, suggesting peptide-LPS and peptide-LTA interactions are less important in their mechanisms of action. Moreover, immunoprecipitation of KAMPs-bacterial factor complexes indicated that membrane surface lipoprotein SlyB and intracellular machineries NQR sodium pump and ribosomes are potential molecular targets for the peptides. Results of this study improve our understanding of the bactericidal function of epithelial cytokeratin fragments, and highlight an unexplored class of human antimicrobial peptides, which may serve as non-alphabeta peptide scaffolds for the design of novel peptide-based antibiotics.
		+
		+	Membrane-Active Epithelial Keratin 6A Fragments (KAMPs) Are Unique Human Antimicrobial Peptides with a Non-alphabeta Structure.,Lee JT, Wang G, Tam YT, Tam C Front Microbiol. 2016 Nov 11;7:1799. eCollection 2016. PMID:27891122<ref>PMID:27891122</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ki0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:55, 10 December 2016

OCA — Sat, 10 Dec 2016 10:55:50 GMT

←Older revision		Revision as of 10:55, 10 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5ki0 is ON HOLD until Paper Publication~~	+	==NMR structure of human antimicrobial peptide KAMP-19==
-		+	<StructureSection load='5ki0' size='340' side='right' caption='[[5ki0]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
-	~~Authors: Wang, G.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ki0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KI0 FirstGlance]. <br>
-	~~Description:~~ NMR structure of human antimicrobial peptide KAMP-19	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ki0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ki0 OCA], [http://pdbe.org/5ki0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ki0 RCSB], [http://www.ebi.ac.uk/pdbsum/5ki0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ki0 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Wang, G]]		[[Category: Wang, G]]
		+	[[Category: Antimicrobial protein]]
		+	[[Category: Keratin 6a fragment]]
		+	[[Category: Non-alpha beta structure]]

OCA at 00:22, 13 July 2016

OCA — Wed, 13 Jul 2016 00:22:47 GMT

←Older revision		Revision as of 00:22, 13 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ki0 is ON HOLD	+	The entry 5ki0 is ON HOLD until Paper Publication

	Authors: Wang, G.		Authors: Wang, G.

OCA: Protected "5ki0" [edit=sysop:move=sysop]

OCA — Thu, 23 Jun 2016 21:39:15 GMT

Protected "5ki0" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:39, 23 June 2016

OCA: New page: '''Unreleased structure''' The entry 5ki0 is ON HOLD Authors: Wang, G. Description: NMR structure of human antimicrobial peptide KAMP-19 Category: Unreleased Structures [[Category:...

OCA — Thu, 23 Jun 2016 21:39:14 GMT

New page: '''Unreleased structure''' The entry 5ki0 is ON HOLD Authors: Wang, G. Description: NMR structure of human antimicrobial peptide KAMP-19 Category: Unreleased Structures [[Category:...

New page

'''Unreleased structure'''

The entry 5ki0 is ON HOLD

Authors: Wang, G.

Description: NMR structure of human antimicrobial peptide KAMP-19
[[Category: Unreleased Structures]]
[[Category: Wang, G]]