5kk1 - Revision history

OCA at 10:02, 27 September 2023

OCA — Wed, 27 Sep 2023 10:02:20 GMT

←Older revision		Revision as of 10:02, 27 September 2023
Line 3:		Line 3:
	<StructureSection load='5kk1' size='340' side='right'caption='[[5kk1]], [[Resolution\|resolution]] 3.38Å' scene=''>		<StructureSection load='5kk1' size='340' side='right'caption='[[5kk1]], [[Resolution\|resolution]] 3.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kk1]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Sulfolobus_sp.~~_nob8h2~~ Sulfolobus sp. ~~nob8h2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5KK1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kk1]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_sp._NOB8H2 Sulfolobus sp. NOB8H2] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KK1 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5k5r\|5k5r]]~~, [[~~5k5q~~\|~~5k5q]], [[5k1y\|5k1y~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.38Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5kk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk1 OCA], [~~http~~://pdbe.org/5kk1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kk1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kk1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk1 OCA], [https://pdbe.org/5kk1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kk1 RCSB], [https://www.ebi.ac.uk/pdbsum/5kk1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk1 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/O93706_9CREN O93706_9CREN]
-	Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.	+
-		+
-	Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.,Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/~~science.aaa9046. PMID:26339031<ref>PMID:26339031<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5kk1" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Sulfolobus sp. ~~nob8h2~~]]	+	[[Category: Sulfolobus sp. NOB8H2]]
-	[[Category: ~~Schumacher, M~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Aspa]]~~	+	[[Category: Schumacher M]]
-	~~[[Category: Partition]]~~	+
-	~~[[Category: Pnob8]]~~	+
-	~~[[Category: Segregation]]~~	+
-	~~[[Category: Transcription-dna complex~~]]	+

OCA at 15:27, 8 July 2020

OCA — Wed, 08 Jul 2020 15:27:15 GMT

←Older revision		Revision as of 15:27, 8 July 2020
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	==Structure of pNOB8 AspA-DNA complex.==		==Structure of pNOB8 AspA-DNA complex.==
-	<StructureSection load='5kk1' size='340' side='right' caption='[[5kk1]], [[Resolution\|resolution]] 3.38Å' scene=''>	+	<StructureSection load='5kk1' size='340' side='right'caption='[[5kk1]], [[Resolution\|resolution]] 3.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kk1]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KK1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kk1]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_sp._nob8h2 Sulfolobus sp. nob8h2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KK1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k5r\|5k5r]], [[5k5q\|5k5q]], [[5k1y\|5k1y]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k5r\|5k5r]], [[5k5q\|5k5q]], [[5k1y\|5k1y]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk1 OCA], [http://pdbe.org/5kk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kk1 RCSB], [http://www.ebi.ac.uk/pdbsum/5kk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5kk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk1 OCA], [http://pdbe.org/5kk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kk1 RCSB], [http://www.ebi.ac.uk/pdbsum/5kk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Sulfolobus sp. nob8h2]]
	[[Category: Schumacher, M]]		[[Category: Schumacher, M]]
	[[Category: Aspa]]		[[Category: Aspa]]

OCA: Protected "5kk1" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2016 02:13:20 GMT

Protected "5kk1" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:13, 13 July 2016

OCA: New page: ==Structure of pNOB8 AspA-DNA complex.== == Structural highlights...

OCA — Wed, 13 Jul 2016 02:13:17 GMT

New page: ==Structure of pNOB8 AspA-DNA complex.== <StructureSection load='5kk1' size='340' side='right' caption='5kk1, resolution 3.38Å' scene=''> == Structural highlights...

New page

==Structure of pNOB8 AspA-DNA complex.==
<StructureSection load='5kk1' size='340' side='right' caption='[[5kk1]], [[Resolution|resolution]] 3.38Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5kk1]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KK1 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k5r|5k5r]], [[5k5q|5k5q]], [[5k1y|5k1y]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk1 OCA], [http://pdbe.org/5kk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kk1 RCSB], [http://www.ebi.ac.uk/pdbsum/5kk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk1 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.,Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/science.aaa9046. PMID:26339031<ref>PMID:26339031</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5kk1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Schumacher, M]]
[[Category: Aspa]]
[[Category: Partition]]
[[Category: Pnob8]]
[[Category: Segregation]]
[[Category: Transcription-dna complex]]