5kl1 - Revision history

OCA at 10:02, 27 September 2023

OCA — Wed, 27 Sep 2023 10:02:54 GMT

←Older revision		Revision as of 10:02, 27 September 2023
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	<StructureSection load='5kl1' size='340' side='right'caption='[[5kl1]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='5kl1' size='340' side='right'caption='[[5kl1]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kl1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KL1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5KL1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kl1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KL1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.701Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5kl8\|5kl8]], [[5kla\|5kla]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5kl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kl1 OCA], [~~http~~://pdbe.org/5kl1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kl1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kl1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kl1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kl1 OCA], [https://pdbe.org/5kl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kl1 RCSB], [https://www.ebi.ac.uk/pdbsum/5kl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kl1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PUM_DROME PUM_DROME]] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole.<ref>PMID:1459455</ref> <ref>PMID:1576962</ref> <ref>PMID:9660969</ref> [[http://www.uniprot.org/uniprot/NANOS_DROME NANOS_DROME]] Maternal RNA-binding protein that is required for germ cells proliferation and self-renewal. Acts by forming a complex with pum and brat that regulates translation and mRNA stability. The complex binds to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Controls posterior development. Rescuing factor for the abdominal defect of posterior group mutants. The other posterior group genes are not required for nanos function but rather play a role in localization or distribution of nanos protein.	+	[https://www.uniprot.org/uniprot/PUM_DROME PUM_DROME] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole.<ref>PMID:1459455</ref> <ref>PMID:1576962</ref> <ref>PMID:9660969</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hall~~, T M.T~~]]	+	[[Category: Hall TMT]]
-	[[Category: Qiu, C]]	+	[[Category: Qiu C]]
-	~~[[Category: Rna binding protein-rna complex]]~~	+
-	~~[[Category: Rna-binding protein~~]]	+

OCA at 15:27, 8 July 2020

OCA — Wed, 08 Jul 2020 15:27:46 GMT

←Older revision		Revision as of 15:27, 8 July 2020
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	==Crystal structure of the Pumilio-Nos-hunchback RNA complex==		==Crystal structure of the Pumilio-Nos-hunchback RNA complex==
-	<StructureSection load='5kl1' size='340' side='right' caption='[[5kl1]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<StructureSection load='5kl1' size='340' side='right'caption='[[5kl1]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kl1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KL1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KL1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kl1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KL1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KL1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kl8\|5kl8]], [[5kla\|5kla]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kl8\|5kl8]], [[5kla\|5kla]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kl1 OCA], [http://pdbe.org/5kl1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kl1 RCSB], [http://www.ebi.ac.uk/pdbsum/5kl1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kl1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5kl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kl1 OCA], [http://pdbe.org/5kl1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kl1 RCSB], [http://www.ebi.ac.uk/pdbsum/5kl1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kl1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Hall, T M.T]]		[[Category: Hall, T M.T]]
	[[Category: Qiu, C]]		[[Category: Qiu, C]]
	[[Category: Rna binding protein-rna complex]]		[[Category: Rna binding protein-rna complex]]
	[[Category: Rna-binding protein]]		[[Category: Rna-binding protein]]

OCA at 05:03, 9 September 2016

OCA — Fri, 09 Sep 2016 05:03:53 GMT

←Older revision		Revision as of 05:03, 9 September 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5kl1 is ~~ON HOLD~~ ~~until Jun 23 2018~~	+	==Crystal structure of the Pumilio-Nos-hunchback RNA complex==
		+	<StructureSection load='5kl1' size='340' side='right' caption='[[5kl1]], [[Resolution\|resolution]] 3.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kl1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KL1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KL1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kl8\|5kl8]], [[5kla\|5kla]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kl1 OCA], [http://pdbe.org/5kl1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kl1 RCSB], [http://www.ebi.ac.uk/pdbsum/5kl1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kl1 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PUM_DROME PUM_DROME]] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole.<ref>PMID:1459455</ref> <ref>PMID:1576962</ref> <ref>PMID:9660969</ref> [[http://www.uniprot.org/uniprot/NANOS_DROME NANOS_DROME]] Maternal RNA-binding protein that is required for germ cells proliferation and self-renewal. Acts by forming a complex with pum and brat that regulates translation and mRNA stability. The complex binds to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Controls posterior development. Rescuing factor for the abdominal defect of posterior group mutants. The other posterior group genes are not required for nanos function but rather play a role in localization or distribution of nanos protein.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Collaboration among the multitude of RNA-binding proteins (RBPs) is ubiquitous, yet our understanding of these key regulatory complexes has been limited to single RBPs. We investigated combinatorial translational regulation by Drosophila Pumilio (Pum) and Nanos (Nos), which control development, fertility, and neuronal functions. Our results show how the specificity of one RBP (Pum) is modulated by cooperative RNA recognition with a second RBP (Nos) to synergistically repress mRNAs. Crystal structures of Nos-Pum-RNA complexes reveal that Nos embraces Pum and RNA, contributes sequence-specific contacts, and increases Pum RNA-binding affinity. Nos shifts the recognition sequence and promotes repression complex formation on mRNAs that are not stably bound by Pum alone, explaining the preponderance of sub-optimal Pum sites regulated in vivo. Our results illuminate the molecular mechanism of a regulatory switch controlling crucial gene expression programs, and provide a framework for understanding how the partnering of RBPs evokes changes in binding specificity that underlie regulatory network dynamics.

-	~~Authors: Qiu~~, C., Hall, T.M.T.	+	Drosophila Nanos acts as a molecular clamp that modulates the RNA-binding and repression activities of Pumilio.,Weidmann CA, Qiu C, Arvola RM, Lou TF, Killingsworth J, Campbell ZT, Tanaka Hall TM, Goldstrohm AC Elife. 2016 Aug 2;5. pii: e17096. doi: 10.7554/eLife.17096. PMID:27482653<ref>PMID:27482653</ref>

-	~~Description: Crystal structure~~ of the ~~Pumilio~~-~~Nos~~-~~hunchback RNA complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5kl1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hall, T M.T]]
	[[Category: Qiu, C]]		[[Category: Qiu, C]]
-	[[Category: ~~Hall, T.M.T~~]]	+	[[Category: Rna binding protein-rna complex]]
		+	[[Category: Rna-binding protein]]

OCA at 12:31, 26 July 2016

OCA — Tue, 26 Jul 2016 12:31:08 GMT

←Older revision		Revision as of 12:31, 26 July 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kl1 is ON HOLD	+	The entry 5kl1 is ON HOLD until Jun 23 2018

	Authors: Qiu, C., Hall, T.M.T.		Authors: Qiu, C., Hall, T.M.T.

OCA: Protected "5kl1" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2016 00:30:20 GMT

Protected "5kl1" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:30, 13 July 2016

OCA: New page: '''Unreleased structure''' The entry 5kl1 is ON HOLD Authors: Qiu, C., Hall, T.M.T. Description: Crystal structure of the Pumilio-Nos-hunchback RNA complex [[Category: Unreleased Struc...

OCA — Wed, 13 Jul 2016 00:30:19 GMT

New page: '''Unreleased structure''' The entry 5kl1 is ON HOLD Authors: Qiu, C., Hall, T.M.T. Description: Crystal structure of the Pumilio-Nos-hunchback RNA complex [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 5kl1 is ON HOLD

Authors: Qiu, C., Hall, T.M.T.

Description: Crystal structure of the Pumilio-Nos-hunchback RNA complex
[[Category: Unreleased Structures]]
[[Category: Qiu, C]]
[[Category: Hall, T.M.T]]