5kn0 - Revision history

OCA at 10:03, 27 September 2023

OCA — Wed, 27 Sep 2023 10:03:57 GMT

←Older revision		Revision as of 10:03, 27 September 2023
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	<StructureSection load='5kn0' size='340' side='right'caption='[[5kn0]], [[Resolution\|resolution]] 2.73Å' scene=''>		<StructureSection load='5kn0' size='340' side='right'caption='[[5kn0]], [[Resolution\|resolution]] 2.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kn0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KN0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KN0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kn0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KN0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.729Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kn1\|5kn1]], [[5kn2\|5kn2]], [[5kn3\|5kn3]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kn0 OCA], [~~http~~://pdbe.org/5kn0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kn0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kn0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kn0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kn0 OCA], [https://pdbe.org/5kn0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kn0 RCSB], [https://www.ebi.ac.uk/pdbsum/5kn0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kn0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/Q05JF3_BOVIN Q05JF3_BOVIN]] Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle.[RuleBase:RU000648]	+	[https://www.uniprot.org/uniprot/Q05JF3_BOVIN Q05JF3_BOVIN] Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle.[RuleBase:RU000648]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Byrd, S]]	+	[[Category: Byrd S]]
-	[[Category: Kang, C]]	+	[[Category: Kang C]]
-	[[Category: Lewis~~, K M]]~~	+	[[Category: Lewis KM]]
-	~~[[Category: Calcium]]~~	+
-	~~[[Category: Calsequestrin]]~~	+
-	~~[[Category: Glycosylation]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Polymer~~]]	+

OCA at 12:19, 25 December 2019

OCA — Wed, 25 Dec 2019 12:19:13 GMT

←Older revision		Revision as of 12:19, 25 December 2019
Line 1:		Line 1:

	==Native bovine skeletal calsequestrin, low-Ca2+ form==		==Native bovine skeletal calsequestrin, low-Ca2+ form==
-	<StructureSection load='5kn0' size='340' side='right' caption='[[5kn0]], [[Resolution\|resolution]] 2.73Å' scene=''>	+	<StructureSection load='5kn0' size='340' side='right'caption='[[5kn0]], [[Resolution\|resolution]] 2.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5kn0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KN0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5kn0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KN0 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
	[[Category: Byrd, S]]		[[Category: Byrd, S]]
	[[Category: Kang, C]]		[[Category: Kang, C]]

OCA at 21:33, 5 October 2016

OCA — Wed, 05 Oct 2016 21:33:28 GMT

←Older revision		Revision as of 21:33, 5 October 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5kn0 is ~~ON HOLD until Paper~~ Publication	+	==Native bovine skeletal calsequestrin, low-Ca2+ form==
		+	<StructureSection load='5kn0' size='340' side='right' caption='[[5kn0]], [[Resolution\|resolution]] 2.73Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kn0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KN0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kn1\|5kn1]], [[5kn2\|5kn2]], [[5kn3\|5kn3]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kn0 OCA], [http://pdbe.org/5kn0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kn0 RCSB], [http://www.ebi.ac.uk/pdbsum/5kn0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kn0 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q05JF3_BOVIN Q05JF3_BOVIN]] Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle.[RuleBase:RU000648]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Calsequestrin is glycosylated and phosphorylated during its transit to its final destination in the junctional sarcoplasmic reticulum. To determine the significance and universal profile of these post-translational modifications to mammalian calsequestrin, we characterized, via mass spectrometry, the glycosylation and phosphorylation of skeletal muscle calsequestrin from cattle (B. taurus), lab mice (M. musculus) and lab rats (R. norvegicus) and cardiac muscle calsequestrin from cattle, lab rats and humans. On average, glycosylation of skeletal calsequestrin consisted of two N-acetylglucosamines and one mannose (GlcNAc(2)Man(1)), while cardiac calsequestrin had five additional mannoses (GlcNAc(2)Man(6)). Skeletal calsequestrin was not phosphorylated, while the C-terminal tails of cardiac calsequestrin contained between zero to two phosphoryls, indicating that phosphorylation of cardiac calsequestrin may be heterogeneous in vivo. Static light scattering experiments showed that the Ca(2+)-dependent polymerization capabilities of native bovine skeletal calsequestrin are enhanced, relative to the non-glycosylated, recombinant isoform, which our crystallographic studies suggest may be due to glycosylation providing a dynamic "guiderail"-like scaffold for calsequestrin polymerization. Glycosylation likely increases a polymerization/depolymerization response to changing Ca(2+) concentrations, and proper glycosylation, in turn, guarantees both effective Ca(2+) storage/buffering of the sarcoplasmic reticulum and localization of calsequestrin (Casq) at its target site.

-	~~Authors~~:	+	Characterization of Post-Translational Modifications to Calsequestrins of Cardiac and Skeletal Muscle.,Lewis KM, Munske GR, Byrd SS, Kang J, Cho HJ, Rios E, Kang C Int J Mol Sci. 2016 Sep 13;17(9). pii: E1539. doi: 10.3390/ijms17091539. PMID:27649144<ref>PMID:27649144</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5kn0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
		+	[[Category: Byrd, S]]
		+	[[Category: Kang, C]]
		+	[[Category: Lewis, K M]]
		+	[[Category: Calcium]]
		+	[[Category: Calsequestrin]]
		+	[[Category: Glycosylation]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Polymer]]

OCA: Protected "5kn0" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2016 00:35:12 GMT

Protected "5kn0" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:35, 13 July 2016

OCA: New page: '''Unreleased structure''' The entry 5kn0 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 Jul 2016 00:35:11 GMT

New page: '''Unreleased structure''' The entry 5kn0 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5kn0 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]