5kti - Revision history

OCA at 10:54, 27 September 2023

OCA — Wed, 27 Sep 2023 10:54:03 GMT

←Older revision		Revision as of 10:54, 27 September 2023
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	<StructureSection load='5kti' size='340' side='right'caption='[[5kti]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5kti' size='340' side='right'caption='[[5kti]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kti]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KTI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5KTI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kti]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KTI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6X6:2,3-DIMETHOXYBENZOIC+ACID'>6X6</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat">[[5kth\|5kth]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6X6:2,3-DIMETHOXYBENZOIC+ACID'>6X6</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PRD_900006:trehalose'>PRD_900006</scene></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">CLEC4E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kti OCA], [https://pdbe.org/5kti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kti RCSB], [https://www.ebi.ac.uk/pdbsum/5kti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kti ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5kti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kti OCA], [~~http~~://pdbe.org/5kti PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kti RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kti PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kti ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/E1BHM0_BOVIN E1BHM0_BOVIN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Djurhuus, R]]	+	[[Category: Djurhuus R]]
-	[[Category: Drickamer, K]]	+	[[Category: Drickamer K]]
-	[[Category: Feinberg, H]]	+	[[Category: Feinberg H]]
-	[[Category: Jacobsen~~, K M~~]]	+	[[Category: Jacobsen KM]]
-	[[Category: Jegouzo~~, S A.F~~]]	+	[[Category: Jegouzo SAF]]
-	[[Category: Poulsen~~, T B~~]]	+	[[Category: Poulsen TB]]
-	[[Category: Rambaruth~~, N D.S~~]]	+	[[Category: Rambaruth NDS]]
-	[[Category: Taylor~~, M E~~]]	+	[[Category: Taylor ME]]
-	[[Category: Weis~~, W I]]~~	+	[[Category: Weis WI]]
-	~~[[Category: C-type lectin]]~~	+
-	~~[[Category: Carbohydrate-binding protein]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Glycobiology]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 06:34, 5 August 2020

OCA — Wed, 05 Aug 2020 06:34:00 GMT

←Older revision		Revision as of 06:34, 5 August 2020
Line 1:		Line 1:

	==Structure of cow mincle complexed with KMJ1==		==Structure of cow mincle complexed with KMJ1==
-	<StructureSection load='5kti' size='340' side='right' caption='[[5kti]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5kti' size='340' side='right'caption='[[5kti]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kti]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KTI OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KTI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kti]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KTI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KTI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6X6:2,3-DIMETHOXYBENZOIC+ACID'>6X6</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=~~PGE~~:~~TRIETHYLENE+GLYCOL~~'>~~PGE~~</scene>, <scene name='pdbligand=~~TRE~~:~~TREHALOSE~~'>~~TRE~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6X6:2,3-DIMETHOXYBENZOIC+ACID'>6X6</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kth\|5kth]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kth\|5kth]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kti OCA], [http://pdbe.org/5kti PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kti RCSB], [http://www.ebi.ac.uk/pdbsum/5kti PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kti ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLEC4E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5kti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kti OCA], [http://pdbe.org/5kti PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kti RCSB], [http://www.ebi.ac.uk/pdbsum/5kti PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kti ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: Djurhuus, R]]		[[Category: Djurhuus, R]]
	[[Category: Drickamer, K]]		[[Category: Drickamer, K]]

OCA at 13:59, 10 September 2016

OCA — Sat, 10 Sep 2016 13:59:20 GMT

←Older revision		Revision as of 13:59, 10 September 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5kti is ~~ON HOLD until Paper~~ Publication	+	==Structure of cow mincle complexed with KMJ1==
		+	<StructureSection load='5kti' size='340' side='right' caption='[[5kti]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kti]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KTI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KTI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6X6:2,3-DIMETHOXYBENZOIC+ACID'>6X6</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kth\|5kth]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kti OCA], [http://pdbe.org/5kti PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kti RCSB], [http://www.ebi.ac.uk/pdbsum/5kti PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kti ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The macrophage receptor mincle binds to trehalose dimycolate on the surface of Mycobacterium tuberculosis. Signaling initiated by this interaction leads to cytokine production, which underlies the ability of mycobacteria to evade the immune system and also to function as adjuvants. In previous work, the mechanism for binding of the sugar headgroup of trehalose dimycolate to mincle has been elucidated, but the basis for enhanced binding to glycolipid ligands, in which hydrophobic substituents are attached to the 6-hydroxyl groups, has been the subject of speculation. In the work reported here, the interaction of trehalose derivatives with bovine mincle has been probed with a series of synthetic mimics of trehalose dimycolate in binding assays, in structural studies by x-ray crystallography, and by site-directed mutagenesis. Binding studies reveal that, rather than reflecting specific structural preference, the apparent affinity of mincle for ligands with hydrophobic substituents correlates with their overall size. Structural and mutagenesis analysis provides evidence for interaction of the hydrophobic substituents with multiple different portions of the surface of mincle and confirms the presence of three Ca2+-binding sites. The structure of an extended portion of the extracellular domain of mincle, beyond the minimal C-type carbohydrate-recognition domain, also constrains the way that the binding domains may interact on the surface of macrophages.

-	~~Authors: Feinberg~~, H., Rambaruth~~, N.D.S.~~, Jegouzo~~, S.A.F.~~, Jacobsen~~, K.M.~~, Djurhuus, R., Poulsen, ~~T.B.~~, Taylor~~, M.E.~~, Drickamer, K.~~, Weis, W~~.I.	+	Binding sites for acylated trehalose analogs of glycolipid ligands on an extended carbohydrate-recognition domain of the macrophage receptor mincle.,Feinberg H, Rambaruth ND, Jegouzo SA, Jacobsen KM, Djurhuus R, Poulsen TB, Weis WI, Taylor ME, Drickamer K J Biol Chem. 2016 Aug 19. pii: jbc.M116.749515. PMID:27542410<ref>PMID:27542410</ref>

-	~~Description: Structure~~ of ~~cow mincle complexed with KMJ1~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Feinberg, H]]~~	+	<div class="pdbe-citations 5kti" style="background-color:#fffaf0;"></div>
-	~~[[Category: Rambaruth, N.D.S]]~~	+	== References ==
-	~~[[Category: Taylor, M.E]]~~	+	<references/>
-	~~[[Category: Poulsen, T.B]]~~	+	__TOC__
		+	</StructureSection>
	[[Category: Djurhuus, R]]		[[Category: Djurhuus, R]]
-	[[Category: Jacobsen, K.M]]
	[[Category: Drickamer, K]]		[[Category: Drickamer, K]]
-	[[Category: ~~Weis~~, ~~W.I~~]]	+	[[Category: Feinberg, H]]
-	[[Category: Jegouzo, S.A.F]]	+	[[Category: Jacobsen, K M]]
		+	[[Category: Jegouzo, S A.F]]
		+	[[Category: Poulsen, T B]]
		+	[[Category: Rambaruth, N D.S]]
		+	[[Category: Taylor, M E]]
		+	[[Category: Weis, W I]]
		+	[[Category: C-type lectin]]
		+	[[Category: Carbohydrate-binding protein]]
		+	[[Category: Complex]]
		+	[[Category: Glycobiology]]
		+	[[Category: Sugar binding protein]]

OCA at 02:02, 4 August 2016

OCA — Thu, 04 Aug 2016 02:02:21 GMT

←Older revision		Revision as of 02:02, 4 August 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kti is ON HOLD	+	The entry 5kti is ON HOLD until Paper Publication

	Authors: Feinberg, H., Rambaruth, N.D.S., Jegouzo, S.A.F., Jacobsen, K.M., Djurhuus, R., Poulsen, T.B., Taylor, M.E., Drickamer, K., Weis, W.I.		Authors: Feinberg, H., Rambaruth, N.D.S., Jegouzo, S.A.F., Jacobsen, K.M., Djurhuus, R., Poulsen, T.B., Taylor, M.E., Drickamer, K., Weis, W.I.

OCA: Protected "5kti" [edit=sysop:move=sysop]

OCA — Tue, 26 Jul 2016 12:53:30 GMT

Protected "5kti" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:53, 26 July 2016

OCA: New page: '''Unreleased structure''' The entry 5kti is ON HOLD Authors: Feinberg, H., Rambaruth, N.D.S., Jegouzo, S.A.F., Jacobsen, K.M., Djurhuus, R., Poulsen, T.B., Taylor, M.E., Drickamer, K.,...

OCA — Tue, 26 Jul 2016 12:53:28 GMT

New page: '''Unreleased structure''' The entry 5kti is ON HOLD Authors: Feinberg, H., Rambaruth, N.D.S., Jegouzo, S.A.F., Jacobsen, K.M., Djurhuus, R., Poulsen, T.B., Taylor, M.E., Drickamer, K.,...

New page

'''Unreleased structure'''

The entry 5kti is ON HOLD

Authors: Feinberg, H., Rambaruth, N.D.S., Jegouzo, S.A.F., Jacobsen, K.M., Djurhuus, R., Poulsen, T.B., Taylor, M.E., Drickamer, K., Weis, W.I.

Description: Structure of cow mincle complexed with KMJ1
[[Category: Unreleased Structures]]
[[Category: Feinberg, H]]
[[Category: Rambaruth, N.D.S]]
[[Category: Taylor, M.E]]
[[Category: Poulsen, T.B]]
[[Category: Djurhuus, R]]
[[Category: Jacobsen, K.M]]
[[Category: Drickamer, K]]
[[Category: Weis, W.I]]
[[Category: Jegouzo, S.A.F]]