5kue - Revision history

OCA at 10:54, 27 September 2023

OCA — Wed, 27 Sep 2023 10:54:55 GMT

←Older revision		Revision as of 10:54, 27 September 2023
Line 3:		Line 3:
	<StructureSection load='5kue' size='340' side='right'caption='[[5kue]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='5kue' size='340' side='right'caption='[[5kue]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kue]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KUE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kue]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KUE FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=EDO:1,2~~-~~ETHANEDIOL'>EDO</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-~~1~~,3-DIOL'>TRS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<~~tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[5kuj\|5kuj]]~~, ~~[[5kui\|5kui]], [[5kug\|5kug]]~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kue OCA], [https://pdbe.org/5kue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kue RCSB], [https://www.ebi.ac.uk/pdbsum/5kue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kue ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">MCU, C10orf42, CCDC109A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kue OCA], [~~http~~://pdbe.org/5kue PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kue RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kue PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kue ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MCU_HUMAN MCU_HUMAN]] Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Activity is regulated by MICU1 and MICU2 that stimulate and inhibit MCU activity, respectively. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes.<ref>PMID:21685886</ref> <ref>PMID:21685888</ref> <ref>PMID:22822213</ref> <ref>PMID:22829870</ref> <ref>PMID:22904319</ref> <ref>PMID:23101630</ref> <ref>PMID:23178883</ref> <ref>PMID:24332854</ref> <ref>PMID:24560927</ref>	+	[https://www.uniprot.org/uniprot/MCU_HUMAN MCU_HUMAN] Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Activity is regulated by MICU1 and MICU2 that stimulate and inhibit MCU activity, respectively. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes.<ref>PMID:21685886</ref> <ref>PMID:21685888</ref> <ref>PMID:22822213</ref> <ref>PMID:22829870</ref> <ref>PMID:22904319</ref> <ref>PMID:23101630</ref> <ref>PMID:23178883</ref> <ref>PMID:24332854</ref> <ref>PMID:24560927</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5kue" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5kue" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Mitochondrial calcium uniporter\|Mitochondrial calcium uniporter]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Junop~~, M S~~]]	+	[[Category: Junop MS]]
-	[[Category: Lee~~, S K~~]]	+	[[Category: Lee SK]]
-	[[Category: Mok~~, C Y.M~~]]	+	[[Category: Mok CYM]]
-	[[Category: Stathopulos~~, P B]]~~	+	[[Category: Stathopulos PB]]
-	~~[[Category: Beta-grasp fold i141m/l146m se-met double mutant n-terminal domain residues 72-189 mitochondrial calcium uniporter]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 08:11, 8 January 2020

OCA — Wed, 08 Jan 2020 08:11:42 GMT

←Older revision		Revision as of 08:11, 8 January 2020
Line 1:		Line 1:

	==Human SeMet incorporated I141M/L146M mitochondrial calcium uniporter (residues 72-189) crystal structure with magnesium==		==Human SeMet incorporated I141M/L146M mitochondrial calcium uniporter (residues 72-189) crystal structure with magnesium==
-	<StructureSection load='5kue' size='340' side='right' caption='[[5kue]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='5kue' size='340' side='right'caption='[[5kue]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kue]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KUE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kue]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KUE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kuj\|5kuj]], [[5kui\|5kui]], [[5kug\|5kug]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kuj\|5kuj]], [[5kui\|5kui]], [[5kug\|5kug]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCU, C10orf42, CCDC109A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kue OCA], [http://pdbe.org/5kue PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kue RCSB], [http://www.ebi.ac.uk/pdbsum/5kue PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kue ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kue OCA], [http://pdbe.org/5kue PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kue RCSB], [http://www.ebi.ac.uk/pdbsum/5kue PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kue ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Junop, M S]]		[[Category: Junop, M S]]
	[[Category: Lee, S K]]		[[Category: Lee, S K]]

OCA at 21:12, 10 September 2016

OCA — Sat, 10 Sep 2016 21:12:39 GMT

←Older revision		Revision as of 21:12, 10 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5kue is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Human SeMet incorporated I141M/L146M mitochondrial calcium uniporter (residues 72-189) crystal structure with magnesium==
		+	<StructureSection load='5kue' size='340' side='right' caption='[[5kue]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kue]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KUE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kuj\|5kuj]], [[5kui\|5kui]], [[5kug\|5kug]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kue OCA], [http://pdbe.org/5kue PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kue RCSB], [http://www.ebi.ac.uk/pdbsum/5kue PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kue ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCU_HUMAN MCU_HUMAN]] Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Activity is regulated by MICU1 and MICU2 that stimulate and inhibit MCU activity, respectively. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes.<ref>PMID:21685886</ref> <ref>PMID:21685888</ref> <ref>PMID:22822213</ref> <ref>PMID:22829870</ref> <ref>PMID:22904319</ref> <ref>PMID:23101630</ref> <ref>PMID:23178883</ref> <ref>PMID:24332854</ref> <ref>PMID:24560927</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Calcium (Ca2+) flux into the matrix is tightly controlled by the mitochondrial Ca2+ uniporter (MCU) due to vital roles in cell death and bioenergetics. However, the precise atomic mechanisms of MCU regulation remain unclear. Here, we solved the crystal structure of the N-terminal matrix domain of human MCU, revealing a beta-grasp-like fold with a cluster of negatively charged residues that interacts with divalent cations. Binding of Ca2+ or Mg2+ destabilizes and shifts the self-association equilibrium of the domain toward monomer. Mutational disruption of the acidic face weakens oligomerization of the isolated matrix domain and full-length human protein similar to cation binding and markedly decreases MCU activity. Moreover, mitochondrial Mg2+ loading or blockade of mitochondrial Ca2+ extrusion suppresses MCU Ca2+-uptake rates. Collectively, our data reveal that the beta-grasp-like matrix region harbors an MCU-regulating acidic patch that inhibits human MCU activity in response to Mg2+ and Ca2+ binding.

-	~~Authors~~:	+	Structural Insights into Mitochondrial Calcium Uniporter Regulation by Divalent Cations.,Lee SK, Shanmughapriya S, Mok MC, Dong Z, Tomar D, Carvalho E, Rajan S, Junop MS, Madesh M, Stathopulos PB Cell Chem Biol. 2016 Aug 24. pii: S2451-9456(16)30242-2. doi:, 10.1016/j.chembiol.2016.07.012. PMID:27569754<ref>PMID:27569754</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5kue" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Junop, M S]]
		+	[[Category: Lee, S K]]
		+	[[Category: Mok, C Y.M]]
		+	[[Category: Stathopulos, P B]]
		+	[[Category: Beta-grasp fold i141m/l146m se-met double mutant n-terminal domain residues 72-189 mitochondrial calcium uniporter]]
		+	[[Category: Transport protein]]

OCA at 13:22, 27 July 2016

OCA — Wed, 27 Jul 2016 13:22:23 GMT

←Older revision		Revision as of 13:22, 27 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kue is ON HOLD	+	The entry 5kue is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5kue" [edit=sysop:move=sysop]

OCA — Tue, 26 Jul 2016 12:56:13 GMT

Protected "5kue" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:56, 26 July 2016

OCA: New page: '''Unreleased structure''' The entry 5kue is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Tue, 26 Jul 2016 12:56:12 GMT

New page: '''Unreleased structure''' The entry 5kue is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5kue is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]