5kyt - Revision history

OCA at 12:45, 4 October 2023

OCA — Wed, 04 Oct 2023 12:45:13 GMT

←Older revision		Revision as of 12:45, 4 October 2023
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	<StructureSection load='5kyt' size='340' side='right'caption='[[5kyt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5kyt' size='340' side='right'caption='[[5kyt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Common_eastern_firefly Common eastern firefly~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KYT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KYT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=EDO:1,2~~-~~ETHANEDIOL'>EDO</scene>~~, ~~<scene name='pdbligand=SLU:5-O-~~[~~N-(DEHYDROLUCIFERYL)-SULFAMOYL~~]~~+ADENOSINE'>SLU</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.001Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http~~:~~//en.wikipedia.org/wiki/Photinus~~-~~luciferin_4~~-~~monooxygenase_(ATP~~-~~hydrolyzing) Photinus-luciferin 4-monooxygenase~~ (~~ATP-hydrolyzing~~)~~], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7~~] </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SLU:5-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL]+ADENOSINE'>SLU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kyt OCA], [~~http~~://pdbe.org/5kyt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kyt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kyt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kyt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kyt OCA], [https://pdbe.org/5kyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kyt RCSB], [https://www.ebi.ac.uk/pdbsum/5kyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kyt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm.	+	[https://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY] Produces green light with a wavelength of 562 nm.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Common eastern firefly]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gulick, A M~~]]	+	[[Category: Photinus pyralis]]
-	[[Category: ~~Adenylation forming enzyme]]~~	+	[[Category: Gulick AM]]
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:49, 27 November 2019

OCA — Wed, 27 Nov 2019 07:49:17 GMT

←Older revision		Revision as of 07:49, 27 November 2019
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	==Structure of Photinus pyralis Luciferase red light emitting variant==		==Structure of Photinus pyralis Luciferase red light emitting variant==
-	<StructureSection load='5kyt' size='340' side='right' caption='[[5kyt]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5kyt' size='340' side='right'caption='[[5kyt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_eastern_firefly Common eastern firefly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KYT FirstGlance]. <br>		<table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_eastern_firefly Common eastern firefly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KYT FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 5kyt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5kyt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Luciferase 3D structures\|Luciferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Common eastern firefly]]		[[Category: Common eastern firefly]]
		+	[[Category: Large Structures]]
	[[Category: Gulick, A M]]		[[Category: Gulick, A M]]
	[[Category: Adenylation forming enzyme]]		[[Category: Adenylation forming enzyme]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 18:33, 15 November 2017

OCA — Wed, 15 Nov 2017 18:33:18 GMT

←Older revision		Revision as of 18:33, 15 November 2017
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	<StructureSection load='5kyt' size='340' side='right' caption='[[5kyt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5kyt' size='340' side='right' caption='[[5kyt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KYT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_eastern_firefly Common eastern firefly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KYT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SLU:5-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL]+ADENOSINE'>SLU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SLU:5-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL]+ADENOSINE'>SLU</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Common eastern firefly]]
	[[Category: Gulick, A M]]		[[Category: Gulick, A M]]
	[[Category: Adenylation forming enzyme]]		[[Category: Adenylation forming enzyme]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 21:29, 22 December 2016

OCA — Thu, 22 Dec 2016 21:29:08 GMT

←Older revision		Revision as of 21:29, 22 December 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5kyt is ~~ON HOLD until Paper~~ Publication	+	==Structure of Photinus pyralis Luciferase red light emitting variant==
		+	<StructureSection load='5kyt' size='340' side='right' caption='[[5kyt]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kyt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KYT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SLU:5-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL]+ADENOSINE'>SLU</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kyt OCA], [http://pdbe.org/5kyt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kyt RCSB], [http://www.ebi.ac.uk/pdbsum/5kyt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kyt ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Unlike the enchanting yellow-green flashes of light produced on warm summer evenings by Photinus pyralis, the most common firefly species in North America, the orange lights of Photinus scintillans are infrequently observed. These Photinus species, and likely all bioluminescent beetles, use the same substrates beetle luciferin, ATP, and oxygen to produce light. It is the structure of the particular luciferase enzyme that is the key to determining the color of the emitted light. We report here the molecular cloning of the P. scintillans luc gene and the expression and characterization of the corresponding novel recombinant luciferase enzyme. A comparison of the amino acid sequence with that of the highly similar P. pyralis enzyme and subsequent mutagenesis studies revealed that the single conservative amino acid change tyrosine to phenylalanine at position 255 accounted for the entire emission color difference. Additional mutagenesis and crystallographic studies were performed on a H-bond network, which includes the position 255 residue and 5 other stringently conserved beetle luciferase residues, that is proximal to the substrate/emitter binding site. The results are interpreted in the context of a speculative proposal that this network is key to the understanding of bioluminescence color determination. This article is protected by copyright. All rights reserved.

-	~~Authors: Gulick~~, A.M.	+	Cloning of the Orange Light-Producing Luciferase from Photinus scintillans - A New Proposal on how Bioluminescence Color is Determined.,Branchini BR, Southworth TL, Fontaine DM, Murtiashaw MH, McGurk A, Talukder MH, Qureshi R, Yetil D, Sundlov JA, Gulick AM Photochem Photobiol. 2016 Nov 18. doi: 10.1111/php.12671. PMID:27861940<ref>PMID:27861940</ref>

-	~~Description: Structure~~ of ~~Photinus pyralis Luciferase red light emitting variant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Gulick, A.M~~]]	+	<div class="pdbe-citations 5kyt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gulick, A M]]
		+	[[Category: Adenylation forming enzyme]]
		+	[[Category: Ligase]]
		+	[[Category: Oxidoreductase]]

OCA at 14:27, 9 September 2016

OCA — Fri, 09 Sep 2016 14:27:05 GMT

←Older revision		Revision as of 14:27, 9 September 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kyt is ON HOLD until ~~sometime in the future~~	+	The entry 5kyt is ON HOLD until Paper Publication

-	Authors: Gulick, A.M	+	Authors: Gulick, A.M.

	Description: Structure of Photinus pyralis Luciferase red light emitting variant		Description: Structure of Photinus pyralis Luciferase red light emitting variant
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Gulick, A.M]]		[[Category: Gulick, A.M]]

OCA: Protected "5kyt" [edit=sysop:move=sysop]

OCA — Thu, 04 Aug 2016 02:12:07 GMT

Protected "5kyt" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:12, 4 August 2016

OCA: New page: '''Unreleased structure''' The entry 5kyt is ON HOLD until sometime in the future Authors: Gulick, A.M Description: Structure of Photinus pyralis Luciferase red light emitting variant ...

OCA — Thu, 04 Aug 2016 02:12:06 GMT

New page: '''Unreleased structure''' The entry 5kyt is ON HOLD until sometime in the future Authors: Gulick, A.M Description: Structure of Photinus pyralis Luciferase red light emitting variant ...

New page

'''Unreleased structure'''

The entry 5kyt is ON HOLD until sometime in the future

Authors: Gulick, A.M

Description: Structure of Photinus pyralis Luciferase red light emitting variant
[[Category: Unreleased Structures]]
[[Category: Gulick, A.M]]