OCA at 12:45, 4 October 2023

OCA — Wed, 04 Oct 2023 12:45:47 GMT

←Older revision		Revision as of 12:45, 4 October 2023
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	==Listeria monocytogenes internalin-like protein lmo2027==		==Listeria monocytogenes internalin-like protein lmo2027==
-	<StructureSection load='5kzs' size='340' side='right' caption='[[5kzs]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5kzs' size='340' side='right'caption='[[5kzs]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kzs]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lismo Lismo~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KZS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kzs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KZS FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~lmo2027 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169963 LISMO])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzs OCA], [~~http~~://pdbe.org/5kzs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kzs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kzs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzs OCA], [https://pdbe.org/5kzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kzs RCSB], [https://www.ebi.ac.uk/pdbsum/5kzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8Y5N0_LISMO Q8Y5N0_LISMO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During pregnancy, the placenta protects the fetus against the maternal immune response, as well as bacterial and viral pathogens. Bacterial pathogens that have evolved specific mechanisms of breaching this barrier, such as Listeria monocytogenes, present a unique opportunity for learning how the placenta carries out its protective function. We previously identified the L. monocytogenes protein Internalin P (InlP) as a secreted virulence factor critical for placental infection. Here, we show that InlP, but not the highly similar L. monocytogenes internalin Lmo2027, binds to human afadin (encoded by AF-6), a protein associated with cell-cell junctions. A crystal structure of InlP reveals several unique features, including an extended leucine-rich repeat (LRR) domain with a distinctive Ca2+-binding site. Despite afadin's involvement in the formation of cell-cell junctions, MDCK epithelial cells expressing InlP displayed a decrease in the magnitude of the traction stresses they could exert on deformable substrates, similar to the decrease in traction exhibited by AF-6 knock-out MDCK cells. L. monocytogenes DeltainlP mutants were deficient in their ability to form actin-rich protrusions from the basal face of polarized epithelial monolayers, a necessary step in the crossing of such monolayers (transcytosis). A similar phenotype was observed for bacteria expressing an internal in-frame deletion in inlP (inlP DeltaLRR5) that specifically disrupts its interaction with afadin. However, afadin deletion in the host cells did not rescue the transcytosis defect. We conclude that secreted InlP targets cytosolic afadin to specifically promote L. monocytogenes transcytosis across the basal face of epithelial monolayers, which may contribute to the crossing of the basement membrane during placental infection.
		+
		+	Listeria monocytogenes InlP interacts with afadin and facilitates basement membrane crossing.,Faralla C, Bastounis EE, Ortega FE, Light SH, Rizzuto G, Gao L, Marciano DK, Nocadello S, Anderson WF, Robbins JR, Theriot JA, Bakardjiev AI PLoS Pathog. 2018 May 30;14(5):e1007094. doi: 10.1371/journal.ppat.1007094. , eCollection 2018 May. PMID:29847585<ref>PMID:29847585</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5kzs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lismo~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Anderson, W F~~]]	+	[[Category: Listeria monocytogenes EGD-e]]
-	[[Category: ~~Bakardjiev, A~~]]	+	[[Category: Anderson WF]]
-	[[Category: ~~Structural genomic~~]]	+	[[Category: Bakardjiev A]]
-	[[Category: Cardona-Correa, A]]	+	[[Category: Cardona-Correa A]]
-	[[Category: Faralla, C]]	+	[[Category: Faralla C]]
-	[[Category: Kwon, K]]	+	[[Category: Kwon K]]
-	[[Category: Light~~, S H~~]]	+	[[Category: Light SH]]
-	[[Category: Minasov, G]]	+	[[Category: Minasov G]]
-	[[Category: Nocadello, S]]	+	[[Category: Nocadello S]]
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Leucine-rich repeat]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 04:39, 16 November 2017

OCA — Thu, 16 Nov 2017 04:39:25 GMT

←Older revision		Revision as of 04:39, 16 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5kzs is ~~ON HOLD until Paper Publication~~	+	==Listeria monocytogenes internalin-like protein lmo2027==
-		+	<StructureSection load='5kzs' size='340' side='right' caption='[[5kzs]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5kzs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lismo Lismo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KZS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lmo2027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169963 LISMO])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzs OCA], [http://pdbe.org/5kzs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kzs RCSB], [http://www.ebi.ac.uk/pdbsum/5kzs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzs ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lismo]]
		+	[[Category: Anderson, W F]]
		+	[[Category: Bakardjiev, A]]
		+	[[Category: Structural genomic]]
		+	[[Category: Cardona-Correa, A]]
		+	[[Category: Faralla, C]]
		+	[[Category: Kwon, K]]
		+	[[Category: Light, S H]]
		+	[[Category: Minasov, G]]
		+	[[Category: Nocadello, S]]
		+	[[Category: Csgid]]
		+	[[Category: Leucine-rich repeat]]
		+	[[Category: Unknown function]]

OCA at 13:56, 10 August 2016

OCA — Wed, 10 Aug 2016 13:56:19 GMT

←Older revision		Revision as of 13:56, 10 August 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kzs is ON HOLD	+	The entry 5kzs is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5kzs" [edit=sysop:move=sysop]

OCA — Thu, 04 Aug 2016 02:17:31 GMT

Protected "5kzs" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:17, 4 August 2016

OCA: New page: '''Unreleased structure''' The entry 5kzs is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 04 Aug 2016 02:17:30 GMT

New page: '''Unreleased structure''' The entry 5kzs is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5kzs is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

5kzs - Revision history

OCA at 12:45, 4 October 2023

OCA at 04:39, 16 November 2017

OCA at 13:56, 10 August 2016

OCA: Protected "5kzs" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 5kzs is ON HOLD Authors: Description: Category: Unreleased Structures