5kzy - Revision history

OCA at 12:46, 4 October 2023

OCA — Wed, 04 Oct 2023 12:46:03 GMT

←Older revision		Revision as of 12:46, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5kzy' size='340' side='right'caption='[[5kzy]], [[Resolution\|resolution]] 2.48Å' scene=''>		<StructureSection load='5kzy' size='340' side='right'caption='[[5kzy]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kzy]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~African_clawed_frog African clawed frog~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KZY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kzy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KZY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CY8:CYCLOPAMINE'>CY8</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.484Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5kzv\|5kzv]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Smo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzy OCA], [https://pdbe.org/5kzy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kzy RCSB], [https://www.ebi.ac.uk/pdbsum/5kzy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzy OCA], [~~http~~://pdbe.org/5kzy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kzy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kzy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzy ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q98SW5_XENLA Q98SW5_XENLA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: African clawed frog]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Huang, P~~]]	+	[[Category: Xenopus laevis]]
-	[[Category: ~~Kim, Y~~]]	+	[[Category: Huang P]]
-	[[Category: ~~Salic, A~~]]	+	[[Category: Kim Y]]
-	[[Category: ~~Cysteine-rich domain]]~~	+	[[Category: Salic A]]
-	~~[[Category: Gpcr]]~~	+
-	~~[[Category: Hedgehog signaling]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Sterol~~]]	+

OCA at 12:22, 25 December 2019

OCA — Wed, 25 Dec 2019 12:22:06 GMT

←Older revision		Revision as of 12:22, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of the xenopus Smoothened cysteine-rich domain (CRD) in complex with cyclopamine==		==Crystal structure of the xenopus Smoothened cysteine-rich domain (CRD) in complex with cyclopamine==
-	<StructureSection load='5kzy' size='340' side='right' caption='[[5kzy]], [[Resolution\|resolution]] 2.48Å' scene=''>	+	<StructureSection load='5kzy' size='340' side='right'caption='[[5kzy]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kzy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KZY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kzy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KZY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kzv\|5kzv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kzv\|5kzv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Smo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzy OCA], [http://pdbe.org/5kzy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kzy RCSB], [http://www.ebi.ac.uk/pdbsum/5kzy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzy OCA], [http://pdbe.org/5kzy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kzy RCSB], [http://www.ebi.ac.uk/pdbsum/5kzy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzy ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: African clawed frog]]
		+	[[Category: Large Structures]]
	[[Category: Huang, P]]		[[Category: Huang, P]]
	[[Category: Kim, Y]]		[[Category: Kim, Y]]

OCA at 05:23, 9 September 2016

OCA — Fri, 09 Sep 2016 05:23:45 GMT

←Older revision		Revision as of 05:23, 9 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5kzy is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the xenopus Smoothened cysteine-rich domain (CRD) in complex with cyclopamine==
		+	<StructureSection load='5kzy' size='340' side='right' caption='[[5kzy]], [[Resolution\|resolution]] 2.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kzy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KZY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KZY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kzv\|5kzv]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kzy OCA], [http://pdbe.org/5kzy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kzy RCSB], [http://www.ebi.ac.uk/pdbsum/5kzy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kzy ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In vertebrates, sterols are necessary for Hedgehog signaling, a pathway critical in embryogenesis and cancer. Sterols activate the membrane protein Smoothened by binding its extracellular, cysteine-rich domain (CRD). Major unanswered questions concern the nature of the endogenous, activating sterol and the mechanism by which it regulates Smoothened. We report crystal structures of CRD complexed with sterols and alone, revealing that sterols induce a dramatic conformational change of the binding site, which is sufficient for Smoothened activation and is unique among CRD-containing receptors. We demonstrate that Hedgehog signaling requires sterol binding to Smoothened and define key residues for sterol recognition and activity. We also show that cholesterol itself binds and activates Smoothened. Furthermore, the effect of oxysterols is abolished in Smoothened mutants that retain activation by cholesterol and Hedgehog. We propose that the endogenous Smoothened activator is cholesterol, not oxysterols, and that vertebrate Hedgehog signaling controls Smoothened by regulating its access to cholesterol.

-	~~Authors~~:	+	Cellular Cholesterol Directly Activates Smoothened in Hedgehog Signaling.,Huang P, Nedelcu D, Watanabe M, Jao C, Kim Y, Liu J, Salic A Cell. 2016 Aug 25;166(5):1176-1187.e14. doi: 10.1016/j.cell.2016.08.003. Epub, 2016 Aug 18. PMID:27545348<ref>PMID:27545348</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5kzy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Huang, P]]
		+	[[Category: Kim, Y]]
		+	[[Category: Salic, A]]
		+	[[Category: Cysteine-rich domain]]
		+	[[Category: Gpcr]]
		+	[[Category: Hedgehog signaling]]
		+	[[Category: Signaling protein]]
		+	[[Category: Sterol]]

OCA at 13:56, 10 August 2016

OCA — Wed, 10 Aug 2016 13:56:35 GMT

←Older revision		Revision as of 13:56, 10 August 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kzy is ON HOLD	+	The entry 5kzy is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5kzy" [edit=sysop:move=sysop]

OCA — Thu, 04 Aug 2016 02:18:11 GMT

Protected "5kzy" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:18, 4 August 2016

OCA: New page: '''Unreleased structure''' The entry 5kzy is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 04 Aug 2016 02:18:09 GMT

New page: '''Unreleased structure''' The entry 5kzy is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5kzy is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]