5l0o - Revision history

OCA at 12:46, 4 October 2023

OCA — Wed, 04 Oct 2023 12:46:27 GMT

←Older revision		Revision as of 12:46, 4 October 2023
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	<StructureSection load='5l0o' size='340' side='right'caption='[[5l0o]], [[Resolution\|resolution]] 2.36Å' scene=''>		<StructureSection load='5l0o' size='340' side='right'caption='[[5l0o]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5l0o]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5L0O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5l0o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L0O FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[3i6x\|3i6x]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.36Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">IQGAP1, KIAA0051 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0o OCA], [https://pdbe.org/5l0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l0o RCSB], [https://www.ebi.ac.uk/pdbsum/5l0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0o ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5l0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0o OCA], [~~http~~://pdbe.org/5l0o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5l0o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5l0o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0o ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IQGA1_HUMAN IQGA1_HUMAN]] Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.<ref>PMID:15695813</ref>	+	[https://www.uniprot.org/uniprot/IQGA1_HUMAN IQGA1_HUMAN] Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.<ref>PMID:15695813</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Liu, J]]	+	[[Category: Liu J]]
-	[[Category: Worthylake, D]]	+	[[Category: Worthylake D]]
-	~~[[Category: Calponin homology]]~~	+
-	~~[[Category: Disulfide-bonded dimer]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 12:22, 25 December 2019

OCA — Wed, 25 Dec 2019 12:22:29 GMT

←Older revision		Revision as of 12:22, 25 December 2019
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	==IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditions==		==IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditions==
-	<StructureSection load='5l0o' size='340' side='right' caption='[[5l0o]], [[Resolution\|resolution]] 2.36Å' scene=''>	+	<StructureSection load='5l0o' size='340' side='right'caption='[[5l0o]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5l0o]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L0O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5l0o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L0O FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6x\|3i6x]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6x\|3i6x]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IQGAP1, KIAA0051 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0o OCA], [http://pdbe.org/5l0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l0o RCSB], [http://www.ebi.ac.uk/pdbsum/5l0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0o OCA], [http://pdbe.org/5l0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l0o RCSB], [http://www.ebi.ac.uk/pdbsum/5l0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0o ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Liu, J]]		[[Category: Liu, J]]
	[[Category: Worthylake, D]]		[[Category: Worthylake, D]]

OCA at 03:39, 10 December 2016

OCA — Sat, 10 Dec 2016 03:39:40 GMT

←Older revision		Revision as of 03:39, 10 December 2016
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/IQGA1_HUMAN IQGA1_HUMAN]] Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.<ref>PMID:15695813</ref>		[[http://www.uniprot.org/uniprot/IQGA1_HUMAN IQGA1_HUMAN]] Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.<ref>PMID:15695813</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	IQGAP1 is a multi-domain scaffold protein involved in many cellular processes. We have determined the crystal structure of an N-terminal fragment spanning residues 1-191 (CHDF hereafter) which contains the entire calponin homology domain. The structure of the CHDF is very similar to other Type 3 calponin homology domains like those from calponin, Vav, and the yeast IQGAP1 ortholog Rng2. However, in the crystal two CHDF molecules form a "head-to-head" or parallel dimer through mostly hydrophobic interactions. Binding experiments indicate that the CHDF binds to both F-actin and Ca2+/calmodulin, but binding is mutually exclusive. Based on the structure, two dimer interface substitutions were introduced. While CHDFL157D disrupts the dimer in gel filtration experiments, oxidized CHDFK161C stabilizes the dimer. These results imply that the CHDF forms the same dimer in solution that is seen in the crystal structure. The disulfide-stabilized dimer displays reduced F-actin binding in sedimentation assays, and shows no binding to Ca2+/calmodulin in isothermal titration calorimetry (ITC) experiments indicating that interface residues are utilized for both binding events. The Calmodulin Target Database predicts that residues 93KK94 are important for CaM binding, and indeed the 93EE94 double mutation displays reduced binding to Ca2+/calmodulin in ITC experiments. Our results indicate that the CHDF dimer interface is used for both F-actin and Ca2+/calmodulin binding, and 93KK94, near the interface, are also used for Ca2+/calmodulin binding. These results are also consistent with full-length IQGAP1 forming a parallel homodimer.
		+
		+	The IQGAP1 N-terminus Forms Dimers and the Dimer Interface is Required for Binding F-actin and Ca2+/calmodulin.,Liu J, Kurella VB, Lecour L, Vanagunas T, Worthylake DK Biochemistry. 2016 Oct 31. PMID:27798963<ref>PMID:27798963</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5l0o" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:11, 3 October 2016

OCA — Mon, 03 Oct 2016 17:11:33 GMT

←Older revision		Revision as of 17:11, 3 October 2016
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-	'''Unreleased structure'''

-	~~The entry 5l0o is ON HOLD~~	+	==IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditions==
-		+	<StructureSection load='5l0o' size='340' side='right' caption='[[5l0o]], [[Resolution\|resolution]] 2.36Å' scene=''>
-	~~Authors: Liu, J., Worthylake, D.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5l0o]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L0O FirstGlance]. <br>
-	~~Description:~~ IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditions	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6x\|3i6x]]</td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0o OCA], [http://pdbe.org/5l0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l0o RCSB], [http://www.ebi.ac.uk/pdbsum/5l0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0o ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IQGA1_HUMAN IQGA1_HUMAN]] Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.<ref>PMID:15695813</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Liu, J]]		[[Category: Liu, J]]
	[[Category: Worthylake, D]]		[[Category: Worthylake, D]]
		+	[[Category: Calponin homology]]
		+	[[Category: Disulfide-bonded dimer]]
		+	[[Category: Signaling protein]]

OCA: Protected "5l0o" [edit=sysop:move=sysop]

OCA — Thu, 04 Aug 2016 02:20:31 GMT

Protected "5l0o" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:20, 4 August 2016

OCA: New page: '''Unreleased structure''' The entry 5l0o is ON HOLD Authors: Liu, J., Worthylake, D. Description: IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditio...

OCA — Thu, 04 Aug 2016 02:20:29 GMT

New page: '''Unreleased structure''' The entry 5l0o is ON HOLD Authors: Liu, J., Worthylake, D. Description: IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditio...

New page

'''Unreleased structure'''

The entry 5l0o is ON HOLD

Authors: Liu, J., Worthylake, D.

Description: IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditions
[[Category: Unreleased Structures]]
[[Category: Liu, J]]
[[Category: Worthylake, D]]