5l40 - Revision history

OCA at 16:06, 4 October 2023

OCA — Wed, 04 Oct 2023 16:06:14 GMT

←Older revision		Revision as of 16:06, 4 October 2023
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	<StructureSection load='5l40' size='340' side='right'caption='[[5l40]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5l40' size='340' side='right'caption='[[5l40]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5l40]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L40 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5L40 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5l40]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L40 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5l40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l40 OCA], [~~http~~://pdbe.org/5l40 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5l40 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5l40 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5l40 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l40 OCA], [https://pdbe.org/5l40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l40 RCSB], [https://www.ebi.ac.uk/pdbsum/5l40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l40 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G9VYV4_STRAT G9VYV4_STRAT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Buttner, M J~~]]	+	[[Category: Streptomyces antibioticus]]
-	[[Category: ~~Lawson, D M~~]]	+	[[Category: Buttner MJ]]
-	[[Category: ~~Schafer, M~~]]	+	[[Category: Lawson DM]]
-	[[Category: ~~Stevenson, C E.~~M]]	+	[[Category: Schafer M]]
-	[[Category: ~~Wilkinson, B~~]]	+	[[Category: Stevenson CEM]]
-	[[Category: ~~Dna gyrase inhibitor]]~~	+	[[Category: Wilkinson B]]
-	~~[[Category: Ketoreductase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Short-chain dehydrogenase/reductase]]~~	+
-	~~[[Category: Simocyclinone~~]]	+

OCA at 06:42, 5 August 2020

OCA — Wed, 05 Aug 2020 06:42:59 GMT

←Older revision		Revision as of 06:42, 5 August 2020
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	==polyketide ketoreductase SimC7 - apo crystal form 1==		==polyketide ketoreductase SimC7 - apo crystal form 1==
-	<StructureSection load='5l40' size='340' side='right' caption='[[5l40]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='5l40' size='340' side='right'caption='[[5l40]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5l40]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L40 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5L40 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5l40]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L40 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5L40 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5l40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l40 OCA], [http://pdbe.org/5l40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l40 RCSB], [http://www.ebi.ac.uk/pdbsum/5l40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l40 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5l40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l40 OCA], [http://pdbe.org/5l40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l40 RCSB], [http://www.ebi.ac.uk/pdbsum/5l40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l40 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Buttner, M J]]		[[Category: Buttner, M J]]
	[[Category: Lawson, D M]]		[[Category: Lawson, D M]]

OCA at 21:35, 5 October 2016

OCA — Wed, 05 Oct 2016 21:35:17 GMT

←Older revision		Revision as of 21:35, 5 October 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5l40 is ~~ON HOLD until Paper~~ Publication	+	==polyketide ketoreductase SimC7 - apo crystal form 1==
		+	<StructureSection load='5l40' size='340' side='right' caption='[[5l40]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5l40]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L40 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L40 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l40 OCA], [http://pdbe.org/5l40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l40 RCSB], [http://www.ebi.ac.uk/pdbsum/5l40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l40 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SimC7 is a polyketide ketoreductase involved in biosynthesis of the angucyclinone moiety of the gyrase inhibitor simocyclinone D8 (SD8). SimC7, which belongs to the short-chain dehydrogenase/reductase (SDR) superfamily, catalyzes reduction of the C-7 carbonyl of the angucyclinone, and the resulting hydroxyl is essential for antibiotic activity. SimC7 shares little sequence similarity with characterized ketoreductases, suggesting it might have a distinct mechanism. To investigate this possibility, we determined the structures of SimC7 alone, with NADP(+), and with NADP(+) and the substrate 7-oxo-SD8. These structures show that SimC7 is distinct from previously characterized polyketide ketoreductases, lacking the conserved catalytic triad, including the active-site tyrosine that acts as central acid-base catalyst in canonical SDR proteins. Taken together with functional analyses of active-site mutants, our data suggest that SimC7 catalyzes a substrate-assisted, two-step reaction for reduction of the C-7 carbonyl group involving intramolecular transfer of a substrate-derived proton to generate a phenolate intermediate.

-	~~Authors~~:	+	Substrate-Assisted Catalysis in Polyketide Reduction Proceeds via a Phenolate Intermediate.,Schafer M, Stevenson CE, Wilkinson B, Lawson DM, Buttner MJ Cell Chem Biol. 2016 Sep 22;23(9):1091-7. doi: 10.1016/j.chembiol.2016.07.018., Epub 2016 Sep 8. PMID:27617849<ref>PMID:27617849</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5l40" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Buttner, M J]]
		+	[[Category: Lawson, D M]]
		+	[[Category: Schafer, M]]
		+	[[Category: Stevenson, C E.M]]
		+	[[Category: Wilkinson, B]]
		+	[[Category: Dna gyrase inhibitor]]
		+	[[Category: Ketoreductase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Short-chain dehydrogenase/reductase]]
		+	[[Category: Simocyclinone]]

OCA: Protected "5l40" [edit=sysop:move=sysop]

OCA — Wed, 01 Jun 2016 14:28:32 GMT

Protected "5l40" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:28, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5l40 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Jun 2016 14:28:30 GMT

New page: '''Unreleased structure''' The entry 5l40 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5l40 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]