5l7d - Revision history

OCA at 16:09, 4 October 2023

OCA — Wed, 04 Oct 2023 16:09:54 GMT

←Older revision		Revision as of 16:09, 4 October 2023
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	<StructureSection load='5l7d' size='340' side='right'caption='[[5l7d]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5l7d' size='340' side='right'caption='[[5l7d]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5l7d]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L7D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5L7D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5l7d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L7D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SMO, SMOH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5l7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7d OCA], [~~http~~://pdbe.org/5l7d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5l7d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5l7d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7d OCA], [https://pdbe.org/5l7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l7d RCSB], [https://www.ebi.ac.uk/pdbsum/5l7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SMO_HUMAN SMO_HUMAN]] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.<ref>PMID:19592253</ref>	+	[https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function.[https://www.uniprot.org/uniprot/SMO_HUMAN SMO_HUMAN] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.<ref>PMID:19592253</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Byrne~~, E F.X~~]]	+	[[Category: Byrne EFX]]
-	[[Category: Covey~~, D F~~]]	+	[[Category: Covey DF]]
-	[[Category: Hedger, G]]	+	[[Category: Hedger G]]
-	[[Category: Luchetti, G]]	+	[[Category: Luchetti G]]
-	[[Category: Miller~~, P S~~]]	+	[[Category: Miller PS]]
-	[[Category: Mydock-McGrane, L]]	+	[[Category: Mydock-McGrane L]]
-	[[Category: Nachtergaele, S]]	+	[[Category: Nachtergaele S]]
-	[[Category: Newstead, S]]	+	[[Category: Newstead S]]
-	[[Category: Rambo~~, R P~~]]	+	[[Category: Rambo RP]]
-	[[Category: Rohatgi, R]]	+	[[Category: Rohatgi R]]
-	[[Category: Sansom~~, M S.P~~]]	+	[[Category: Sansom MSP]]
-	[[Category: Siebold, C]]	+	[[Category: Siebold C]]
-	[[Category: Sircar, R]]	+	[[Category: Sircar R]]
-	[[Category: Tully~~, M D]]~~	+	[[Category: Tully MD]]
-	~~[[Category: Cholesterol]]~~	+
-	~~[[Category: G protein coupled receptor]]~~	+
-	~~[[Category: Hedgehog signaling]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Morphogen signaling]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 06:59, 5 August 2020

OCA — Wed, 05 Aug 2020 06:59:29 GMT

←Older revision		Revision as of 06:59, 5 August 2020
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	==Structure of human Smoothened in complex with cholesterol==		==Structure of human Smoothened in complex with cholesterol==
-	<StructureSection load='5l7d' size='340' side='right' caption='[[5l7d]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='5l7d' size='340' side='right'caption='[[5l7d]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5l7d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L7D OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5L7D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5l7d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L7D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5L7D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5l7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7d OCA], [http://pdbe.org/5l7d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l7d RCSB], [http://www.ebi.ac.uk/pdbsum/5l7d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7d ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SMO, SMOH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5l7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7d OCA], [http://pdbe.org/5l7d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l7d RCSB], [http://www.ebi.ac.uk/pdbsum/5l7d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Byrne, E F.X]]		[[Category: Byrne, E F.X]]
	[[Category: Covey, D F]]		[[Category: Covey, D F]]

OCA at 20:39, 3 August 2016

OCA — Wed, 03 Aug 2016 20:39:52 GMT

←Older revision		Revision as of 20:39, 3 August 2016
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SMO_HUMAN SMO_HUMAN]] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.<ref>PMID:19592253</ref>		[[http://www.uniprot.org/uniprot/SMO_HUMAN SMO_HUMAN]] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.<ref>PMID:19592253</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Developmental signals of the Hedgehog (Hh) and Wnt families are transduced across the membrane by Frizzledclass G-protein-coupled receptors (GPCRs) composed of both a heptahelical transmembrane domain (TMD) and an extracellular cysteine-rich domain (CRD). How the large extracellular domains of GPCRs regulate signalling by the TMD is unknown. We present crystal structures of the Hh signal transducer and oncoprotein Smoothened, a GPCR that contains two distinct ligand-binding sites: one in its TMD and one in the CRD. The CRD is stacked a top the TMD, separated by an intervening wedge-like linker domain. Structure-guided mutations show that the interface between the CRD, linker domain and TMD stabilizes the inactive state of Smoothened. Unexpectedly, we find a cholesterol molecule bound to Smoothened in the CRD binding site. Mutations predicted to prevent cholesterol binding impair the ability of Smoothened to transmit native Hh signals. Binding of a clinically used antagonist, vismodegib, to the TMD induces a conformational change that is propagated to the CRD, resulting in loss of cholesterol from the CRD-linker domain-TMD interface. Our results clarify the structural mechanism by which the activity of a GPCR is controlled by ligand-regulated interactions between its extracellular and transmembrane domains.
		+
		+	Structural basis of Smoothened regulation by its extracellular domains.,Byrne EF, Sircar R, Miller PS, Hedger G, Luchetti G, Nachtergaele S, Tully MD, Mydock-McGrane L, Covey DF, Rambo RP, Sansom MS, Newstead S, Rohatgi R Nature. 2016 Jul 28;535(7613):517-22. PMID:27437577<ref>PMID:27437577</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5l7d" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:38, 26 July 2016

OCA — Tue, 26 Jul 2016 15:38:58 GMT

←Older revision		Revision as of 15:38, 26 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5l7d is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Structure of human Smoothened in complex with cholesterol==
-		+	<StructureSection load='5l7d' size='340' side='right' caption='[[5l7d]], [[Resolution\|resolution]] 3.20Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5l7d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L7D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L7D FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7d OCA], [http://pdbe.org/5l7d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l7d RCSB], [http://www.ebi.ac.uk/pdbsum/5l7d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7d ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SMO_HUMAN SMO_HUMAN]] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.<ref>PMID:19592253</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Byrne, E F.X]]
		+	[[Category: Covey, D F]]
		+	[[Category: Hedger, G]]
		+	[[Category: Luchetti, G]]
		+	[[Category: Miller, P S]]
		+	[[Category: Mydock-McGrane, L]]
		+	[[Category: Nachtergaele, S]]
		+	[[Category: Newstead, S]]
		+	[[Category: Rambo, R P]]
		+	[[Category: Rohatgi, R]]
		+	[[Category: Sansom, M S.P]]
		+	[[Category: Siebold, C]]
		+	[[Category: Sircar, R]]
		+	[[Category: Tully, M D]]
		+	[[Category: Cholesterol]]
		+	[[Category: G protein coupled receptor]]
		+	[[Category: Hedgehog signaling]]
		+	[[Category: Membrane protein]]
		+	[[Category: Morphogen signaling]]
		+	[[Category: Signaling protein]]

OCA at 21:48, 23 June 2016

OCA — Thu, 23 Jun 2016 21:48:39 GMT

←Older revision		Revision as of 21:48, 23 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5l7d is ON HOLD	+	The entry 5l7d is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5l7d" [edit=sysop:move=sysop]

OCA — Mon, 20 Jun 2016 13:59:36 GMT

Protected "5l7d" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:59, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5l7d is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Mon, 20 Jun 2016 13:59:34 GMT

New page: '''Unreleased structure''' The entry 5l7d is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5l7d is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]