5la8 - Revision history

OCA at 16:12, 4 October 2023

OCA — Wed, 04 Oct 2023 16:12:58 GMT

←Older revision		Revision as of 16:12, 4 October 2023
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	<StructureSection load='5la8' size='340' side='right'caption='[[5la8]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5la8' size='340' side='right'caption='[[5la8]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5la8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LA8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LA8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5la8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LA8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Lysozyme Lysozyme]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5la8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5la8 OCA], [~~http~~://pdbe.org/5la8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5la8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5la8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5la8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5la8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5la8 OCA], [https://pdbe.org/5la8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5la8 RCSB], [https://www.ebi.ac.uk/pdbsum/5la8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5la8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Castellvi A]]
-	[[Category: Castellvi, A]]	+	[[Category: Juanhuix J]]
-	[[Category: Juanhuix, J]]	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Room temperature~~]]	+

OCA at 07:04, 5 August 2020

OCA — Wed, 05 Aug 2020 07:04:11 GMT

←Older revision		Revision as of 07:04, 5 August 2020
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	==Room temperature X-ray diffraction of tetragonal HEWL. Third data set (0.93 MGy)==		==Room temperature X-ray diffraction of tetragonal HEWL. Third data set (0.93 MGy)==
-	<StructureSection load='5la8' size='340' side='right' caption='[[5la8]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5la8' size='340' side='right'caption='[[5la8]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5la8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LA8 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LA8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5la8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LA8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LA8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5la8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5la8 OCA], [http://pdbe.org/5la8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5la8 RCSB], [http://www.ebi.ac.uk/pdbsum/5la8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5la8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5la8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5la8 OCA], [http://pdbe.org/5la8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5la8 RCSB], [http://www.ebi.ac.uk/pdbsum/5la8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5la8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5la8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5la8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Castellvi, A]]		[[Category: Castellvi, A]]

OCA at 07:30, 18 January 2017

OCA — Wed, 18 Jan 2017 07:30:58 GMT

←Older revision		Revision as of 07:30, 18 January 2017
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>		[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Macromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced by the intense X-ray beam. Several effects, such as aggregation in protein solutions and global and site-specific damage in crystals, reduce the data quality or even introduce artefacts that can result in a biologically misguiding structure. One strategy to reduce these negative effects is the inclusion of an additive in the buffer solution to act as a free radical scavenger. Here the properties of uridine as a scavenger for both SAXS and MX experiments on lysozyme at room temperature are examined. In MX experiments, upon addition of uridine at 1 M, the critical dose D1/2 is increased by a factor of approximately 1.7, a value similar to that obtained in the presence of the most commonly used scavengers such as ascorbate and sodium nitrate. Other figures of merit to assess radiation damage show a similar trend. In SAXS experiments, the scavenging effect of 40 mM uridine is similar to that of 5% v/v glycerol, and greater than 2 mM DTT and 1 mM ascorbic acid. In all cases, the protective effect of uridine is proportional to its concentration.
		+
		+	Uridine as a new scavenger for synchrotron-based structural biology techniques.,Crosas E, Castellvi A, Crespo I, Fulla D, Gil-Ortiz F, Fuertes G, Kamma-Lorger CS, Malfois M, Aranda MA, Juanhuix J J Synchrotron Radiat. 2017 Jan 1;24(Pt 1):53-62. doi: 10.1107/S1600577516018452. , Epub 2017 Jan 1. PMID:28009546<ref>PMID:28009546</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5la8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:43, 22 December 2016

OCA — Thu, 22 Dec 2016 21:43:45 GMT

←Older revision		Revision as of 21:43, 22 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5la8 is ON HOLD until Paper Publication~~	+	==Room temperature X-ray diffraction of tetragonal HEWL. Third data set (0.93 MGy)==
-		+	<StructureSection load='5la8' size='340' side='right' caption='[[5la8]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors: Castellvi, A., Juanhuix, J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5la8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LA8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LA8 FirstGlance]. <br>
-	~~Description:~~ Room temperature X-ray diffraction of tetragonal HEWL. Third data set (0.93 MGy)	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5la8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5la8 OCA], [http://pdbe.org/5la8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5la8 RCSB], [http://www.ebi.ac.uk/pdbsum/5la8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5la8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Lysozyme]]
		+	[[Category: Castellvi, A]]
	[[Category: Juanhuix, J]]		[[Category: Juanhuix, J]]
-	[[Category: ~~Castellvi, A~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Room temperature]]

OCA: Protected "5la8" [edit=sysop:move=sysop]

OCA — Thu, 23 Jun 2016 21:55:34 GMT

Protected "5la8" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:55, 23 June 2016

OCA: New page: '''Unreleased structure''' The entry 5la8 is ON HOLD until Paper Publication Authors: Castellvi, A., Juanhuix, J. Description: Room temperature X-ray diffraction of tetragonal HEWL. Th...

OCA — Thu, 23 Jun 2016 21:55:32 GMT

New page: '''Unreleased structure''' The entry 5la8 is ON HOLD until Paper Publication Authors: Castellvi, A., Juanhuix, J. Description: Room temperature X-ray diffraction of tetragonal HEWL. Th...

New page

'''Unreleased structure'''

The entry 5la8 is ON HOLD until Paper Publication

Authors: Castellvi, A., Juanhuix, J.

Description: Room temperature X-ray diffraction of tetragonal HEWL. Third data set (0.93 MGy)
[[Category: Unreleased Structures]]
[[Category: Juanhuix, J]]
[[Category: Castellvi, A]]