5lb7 - Revision history

OCA at 16:13, 4 October 2023

OCA — Wed, 04 Oct 2023 16:13:46 GMT

←Older revision		Revision as of 16:13, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5lb7' size='340' side='right'caption='[[5lb7]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='5lb7' size='340' side='right'caption='[[5lb7]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lb7]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LB7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lb7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LB7 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Katnb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=10090 LK3 transgenic mice]), Katna1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule~~-~~severing_ATPase Microtubule-severing ATPase]~~, ~~with EC number~~ [~~http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lb7 OCA], [https://pdbe.org/5lb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lb7 RCSB], [https://www.ebi.ac.uk/pdbsum/5lb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lb7 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lb7 OCA], [~~http~~://pdbe.org/5lb7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lb7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lb7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lb7 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KTNB1_MOUSE KTNB1_MOUSE]] Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[HAMAP-Rule:MF_03022] [[http://www.uniprot.org/uniprot/ASPM_MOUSE ASPM_MOUSE]] Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis.<ref>PMID:12355089</ref> <ref>PMID:9819352</ref> [[http://www.uniprot.org/uniprot/KTNA1_MOUSE KTNA1_MOUSE]] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth (By similarity).[HAMAP-Rule:MF_03023]	+	[https://www.uniprot.org/uniprot/KTNB1_MOUSE KTNB1_MOUSE] Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[HAMAP-Rule:MF_03022]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice]]~~	+	[[Category: Mus musculus]]
-	~~[[Category: Microtubule-severing ATPase~~]]	+	[[Category: Capitani G]]
-	[[Category: Capitani, G]]	+	[[Category: Kammerer RA]]
-	[[Category: Kammerer~~, R A~~]]	+	[[Category: Rezabkova L]]
-	[[Category: Rezabkova, L]]	+	[[Category: Steinmetz MO]]
-	[[Category: Steinmetz~~, M O]]~~	+
-	~~[[Category: Aspm]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Katanin]]~~	+
-	~~[[Category: Severing enzyme~~]]	+

OCA at 06:27, 16 October 2019

OCA — Wed, 16 Oct 2019 06:27:36 GMT

←Older revision		Revision as of 06:27, 16 October 2019
Line 1:		Line 1:

	==Complex structure between p60N/p80C katanin and a peptide derived from ASPM==		==Complex structure between p60N/p80C katanin and a peptide derived from ASPM==
-	<StructureSection load='5lb7' size='340' side='right' caption='[[5lb7]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='5lb7' size='340' side='right'caption='[[5lb7]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lb7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lb7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LB7 FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Katnb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Katna1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lb7 OCA], [http://pdbe.org/5lb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lb7 RCSB], [http://www.ebi.ac.uk/pdbsum/5lb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lb7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lb7 OCA], [http://pdbe.org/5lb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lb7 RCSB], [http://www.ebi.ac.uk/pdbsum/5lb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lb7 ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Microtubule-severing ATPase]]		[[Category: Microtubule-severing ATPase]]
	[[Category: Capitani, G]]		[[Category: Capitani, G]]

OCA at 06:18, 4 May 2017

OCA — Thu, 04 May 2017 06:18:15 GMT

←Older revision		Revision as of 06:18, 4 May 2017
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/KTNB1_MOUSE KTNB1_MOUSE]] Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[HAMAP-Rule:MF_03022] [[http://www.uniprot.org/uniprot/ASPM_MOUSE ASPM_MOUSE]] Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis.<ref>PMID:12355089</ref> <ref>PMID:9819352</ref> [[http://www.uniprot.org/uniprot/KTNA1_MOUSE KTNA1_MOUSE]] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth (By similarity).[HAMAP-Rule:MF_03023]		[[http://www.uniprot.org/uniprot/KTNB1_MOUSE KTNB1_MOUSE]] Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[HAMAP-Rule:MF_03022] [[http://www.uniprot.org/uniprot/ASPM_MOUSE ASPM_MOUSE]] Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis.<ref>PMID:12355089</ref> <ref>PMID:9819352</ref> [[http://www.uniprot.org/uniprot/KTNA1_MOUSE KTNA1_MOUSE]] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth (By similarity).[HAMAP-Rule:MF_03023]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ASPM (known as Asp in fly and ASPM-1 in worm) is a microcephaly-associated protein family that regulates spindle architecture, but the underlying mechanism is poorly understood. Here, we show that ASPM forms a complex with another protein linked to microcephaly, the microtubule-severing ATPase katanin. ASPM and katanin localize to spindle poles in a mutually dependent manner and regulate spindle flux. X-ray crystallography revealed that the heterodimer formed by the N- and C-terminal domains of the katanin subunits p60 and p80, respectively, binds conserved motifs in ASPM. Reconstitution experiments demonstrated that ASPM autonomously tracks growing microtubule minus ends and inhibits their growth, while katanin decorates and bends both ends of dynamic microtubules and potentiates the minus-end blocking activity of ASPM. ASPM also binds along microtubules, recruits katanin and promotes katanin-mediated severing of dynamic microtubules. We propose that the ASPM-katanin complex controls microtubule disassembly at spindle poles and that misregulation of this process can lead to microcephaly.
		+
		+	Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex.,Jiang K, Rezabkova L, Hua S, Liu Q, Capitani G, Maarten Altelaar AF, Heck AJR, Kammerer RA, Steinmetz MO, Akhmanova A Nat Cell Biol. 2017 May;19(5):480-492. doi: 10.1038/ncb3511. Epub 2017 Apr 24. PMID:28436967<ref>PMID:28436967</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5lb7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:59, 27 April 2017

OCA — Thu, 27 Apr 2017 13:59:34 GMT

←Older revision		Revision as of 13:59, 27 April 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5lb7 is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Complex structure between p60N/p80C katanin and a peptide derived from ASPM==
-		+	<StructureSection load='5lb7' size='340' side='right' caption='[[5lb7]], [[Resolution\|resolution]] 1.50Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5lb7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LB7 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lb7 OCA], [http://pdbe.org/5lb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lb7 RCSB], [http://www.ebi.ac.uk/pdbsum/5lb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lb7 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KTNB1_MOUSE KTNB1_MOUSE]] Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[HAMAP-Rule:MF_03022] [[http://www.uniprot.org/uniprot/ASPM_MOUSE ASPM_MOUSE]] Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis.<ref>PMID:12355089</ref> <ref>PMID:9819352</ref> [[http://www.uniprot.org/uniprot/KTNA1_MOUSE KTNA1_MOUSE]] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth (By similarity).[HAMAP-Rule:MF_03023]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Microtubule-severing ATPase]]
		+	[[Category: Capitani, G]]
		+	[[Category: Kammerer, R A]]
		+	[[Category: Rezabkova, L]]
		+	[[Category: Steinmetz, M O]]
		+	[[Category: Aspm]]
		+	[[Category: Hydrolase]]
		+	[[Category: Katanin]]
		+	[[Category: Severing enzyme]]

OCA at 10:35, 9 March 2017

OCA — Thu, 09 Mar 2017 10:35:00 GMT

←Older revision		Revision as of 10:35, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5lb7 is ON HOLD	+	The entry 5lb7 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5lb7" [edit=sysop:move=sysop]

OCA — Thu, 23 Jun 2016 21:58:41 GMT

Protected "5lb7" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:58, 23 June 2016

OCA: New page: '''Unreleased structure''' The entry 5lb7 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 23 Jun 2016 21:58:39 GMT

New page: '''Unreleased structure''' The entry 5lb7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5lb7 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]